DOI QR코드

DOI QR Code

Establishment of an analytical method for butaphosphan (BTP), a stress-attenuating agent, and its application in the preliminary pharmacokinetic evaluation of residues in olive flounder Paralichthys olivaceus

  • Lee, Ji-Hoon (Aquatic Disease Control Division, National Institute of Fisheries Science) ;
  • Bae, Jun Sung (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Lee, Chae Won (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Yang, Chan Yeong (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Choi, Ji-Sung (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University) ;
  • Kang, Yue-Jai (Department of Aquatic Life and Medical Sciences, Sun Moon University) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science & Technology, Kunsan National University)
  • Received : 2019.09.05
  • Accepted : 2020.03.02
  • Published : 2020.04.30

Abstract

Background: Butaphosphan (BTP) has recently been introduced into the Korean aquaculture sector as a stressattenuating agent. In this study, a sensitive chemical analytical method was established for the detection of BTP in the olive flounder (Paralichthys olivaceus) tissues. Methods: Utilizing a method employing liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), detection sensitivity, specificity, and precision were satisfactorily established. Temporal changes in the BTP plasma and muscle concentrations were assessed after a single intramuscular injection of BTP (50 and 150 mg/kg) to the olive flounder maintained at 13 ℃ or 22 ℃. Results: High BTP plasma levels were achieved immediately after the injection, and the drug was rapidly eliminated. Additionally, plasma BTP levels were markedly dependent on the elimination rate, which, in turn, seemed dependent on the water temperature, with the drug elimination half-life and mean residence time significantly shorter at 22 ℃ than 13 ℃. Overall, muscle BTP levels were markedly lower than the plasma levels. Notably, muscle levels were not influenced by water temperatures. Muscle BTP concentrations were used to estimate the necessary withdrawal period for drugs used in food fish, with BTP levels maintained far below the possible hazardous limit. Conclusions: In conclusion, the established LC-MS/MS method can be used for BTP residue detection with high sensitivity and reproducibility.

Keywords

References

  1. Alkobaby AI. Effects of maternal injection with organic phosphorus and vitamin $B_{12}$ on reproductive performance and newly hatched offspring of Nile tilapia (Oreochromis niloticus). Proceedings, The 8th International Symposium on Tilapia in Aquaculture, October 12-14, 2008, Cairo, Egypt; cals.arizona.edu/azaqua/ista/ISTA8/FinalPapers. Accessed 20 Jan 2010.
  2. Benez LZ. Pharmacokinetic parameters: which are necessary to define a drug substance? Eur J Respir Dis Supp. 1984;134:45-61.
  3. Deniz A, Spiecker-Hauser U, Rehagen M. Efficacy of a butafosfan and vitamin $B_{12}$ combination ($Catosal^{(R)}$) on biochemical and hematological blood parameters in dogs treated with dexamethasone. Intern J Appl Res Vet Med. 2009;7:116-29.
  4. Du XY. Studies on pharmacy and pharmacokinetics of compound butafosfan. Ph. D Thesis, China Agricultural University, 2004. www.dissertationtopic.net/doc/1578105 (2004). Accessed 20 Jan 2020.
  5. Durisova M. A physiological review and structure of mean residence times. Gen Physiol Biophys. 2014;33:75-80. https://doi.org/10.4149/gpb_2013060
  6. European Medicines Agency. Opinion of the committee for medicinal products for veterinary use on the establishment of maximum residue limits: name of the substance-butafosfan. EMEA/V/MRL/003610/EXTN/0003 (2013). Accessed 20 Jan 2020.
  7. Funaki T. Enterohepatic circulation model for population pharmacokinetic analysis. J Pharm Pharmacol. 1999;51:1143-8. https://doi.org/10.1211/0022357991776831
  8. Furll M, Deniz A, Westphal B, Illing C, Constable PD. Effect of multiple intravenous injections of butaphosphan and cyanocobalamin on the metabolism of periparturient dairy cows. J Dairy Sci. 2010;93:4155-64. https://doi.org/10.3168/jds.2009-2914
  9. Furll M, Wittek T, Gegenbach S, Schmidt B. Effects of preoperative application of butaphosphan and cyanocobalamin on reconvalescence, clinic-chemical parameters, and antioxidative metabolism and postoperative abomasal emptying in cows with abnormal dislocation. Tieraertl Prax. 2006;34:351-6.
  10. Gabrielsson J, Weiner D. Pharmacokinetic and pharmacodynamic data analysis: concepts and applications, 3rd eds. Sweden: Swedish Pharamceutical Press, Stockholm; 2000. p. 645-59.
  11. Hasi S, Du X, Zhu B, Jiang J. Studies on effects of compound butaphosfan solution on endurance capability and energy metabolism in mice. Chin J Anim Vet Sci. 2004;35:290-4.
  12. Hasi S, Jiang J, Du X, Zhu B. Anti-cold stress effects and mechanisms of compound butaphosphan solution. Prog Vet Med. 2005a;26:59-62.
  13. Hasi S, Jiang J, Zhu B, Du X. Studies on anti-heat stress effects and mechanisms of compound butaphosfan solution. Acta Vet Zootech Sin. 2005b;36:1334-8. https://doi.org/10.3321/j.issn:0366-6964.2005.12.017
  14. Hekman P. Withdrawal-time calculation program WT1.4. London, UK: European agency for the evaluation of medicinal products (EMEA); 1996.
  15. Kleinow KM, Jarboe HH, Shomaker HE. Comparative pharmacokinetics and bioavailability of oxolinic acid in channel catfish (Ictalurus punctatus) and rainbow trout (Oncorrhynchus mykiss). Can J Fish Aquat Sci. 1994;51:1205-11. https://doi.org/10.1139/f94-120
  16. Korea Ministry of Food and Drug Administration (2017) Principles of Standard Establishment for Foods. p. 155. https://mfds.go.kr/brd/m_218/list.do?Accessed 20 Jan 2020.
  17. Korea Rural Economic Institute. 2017-Food Balance Sheet, 2017.
  18. Mechesso AF, Kim Y, Park S. Effects of butaphosphan and cyanocovalamin combination on plasma immune and biochemical parameters of olive flounder (Paralichthys olivaceus) subjected to crowding stress. Aquacult Res. 2019;50:1611-7. https://doi.org/10.1111/are.14038
  19. Meyer RE, Fish RE. Pharmacology of injectable anesthetics, sedatives, and tranquilizers. In: Anesthesia and analgesia in laboratory animals, 2nd eds. San Diego: Academic Press; 2008. 27-82.
  20. National Institute of Fisheries Sciences. Aquatic medicine catalogue. http://www.nifs.go.kr/adms/index.ad (2018) Accessed 20 Jan 2010.
  21. Pereira RA, Fensterseifer S, Barcelos VB, Martins CF, Schneider A, Schmitt E, Pfeifer LFM, Del Pino FAB, Correa MN. (2013b) Metabolic parameters and dry matter intake of ewes treated with butaphosphan and cyanocobalamin in the early postpartum period. Small Rum Res. 2013b;114:140-5. https://doi.org/10.1016/j.smallrumres.2013.05.016
  22. Pereira RA, Montagner P, Schneider A, Schmitt E, Rabassa VR, Pfeifer LFM, Del Pino FAB, Pulga ME, Correa MN. Effect of butaphosphan and cyanocobalamin on postpartum metabolism and milk production in dairy cows. Animal. 2013a;7:1143-7. https://doi.org/10.1017/s1751731113000013
  23. Rolling E, Berghaus RD, Rapnicki P, Godden SM, Overton MW. The effect of injectable butaphosphan and cyanocobalamin on postpartum serum betahydroxybutyrate, calcium, and phosphorus concentrations in dairy cattle. J Dairy Sci. 2010;93:978-87. https://doi.org/10.3168/jds.2009-2508
  24. Seo JS, Lee JH, Park JJ, Choi JS, Bae JS, Lee CW, Yang CY, Kang YJ, Choi SH, Park KH. Biochemical and stress-attenuating effects of butaphosphancycnocobalamin combination drug in olive flounder Paralichthys olivaceus. Fish Sci. 2019 (published online December 16). https://doi.org/10.1007/s12562-019-01389-x.
  25. Sun F, Wang J, Yang S, Zhang S, Shen J, Xingyuan C. Pharmacokinetics of butafosfan after intravenous and intramuscular administration in piglets. J Vet Pharmacol Ther. 2016;40:203-5. https://doi.org/10.1111/jvp.12347
  26. Tabeleao VC, Schwegler E, Pereira RA, Krause ART, Montagner P, Feijo JO, Schneider A, Schmitt E, Brauner CC, Rabassa VR, Del Pino FAB, Correa MN. Combined butaphosphan and cyanocobalamin on the glucose metabolism of dairy cows after calving. Arq Bras Med Vet Zootec. 2017;69:317-24. https://doi.org/10.1590/1678-4162-8453
  27. Van de Vis H, Kruijt-Kloosterboer K, Veldman M. Test for potential protective effects of $Catosal^{(R)}$ on mortality of fish due to temporary hypoxia. Internal Report No. C061/04, RIVO-Netherlands Institute for Fisheries Research, Ymuiden, Netherlands. edepot.wur.nl/148440 (2004). Accessed 20 Jan 2020.
  28. Van der Staay DE, Groot J, Van Reenen CC, Hoving-Bolink AH, Schuurman T, Schmidt BH. Effects of butaphosphan on salivary cortisol and behavioral response to social stress in piglets. J Vet Pharmacol Ther. 2007;30:410-6. https://doi.org/10.1111/j.1365-2885.2007.00884.x
  29. Zhang Y, Hou M, Zhou J, Xie S. PKSolver: an add-in program for pharmacokinetic and pharmacodynamics data analysis in Microsoft Excel. Computer Methods Programs Biomed. 2010;99:306-14. https://doi.org/10.1016/j.cmpb.2010.01.007