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Phytochemical Screening, Isolation, Characterization of Bioactive and Biological Activity of Bungkang, (Syzygium polyanthum) Root-bark Essential Oil

  • Umaru, Isaac John (Faculty of Resource Science and Technology, University of Malaysia Sarawak) ;
  • Umaru, Kerenhappuch I. (Department of Biochemistry, University of Maiduguri) ;
  • Umaru, Hauwa A. (Department of Biochemistry Moddibo Adama University of Technology Yola)
  • Received : 2020.04.28
  • Accepted : 2020.05.13
  • Published : 2020.06.30

Abstract

Bungkang (Syzygium polyanthum) is a medium to tall plant which produces medicinal root-bark, the plant is normally found along inland river bank and produces small white flowers and fruits. Essential oils are among the most interesting components of the plant extracts consisting mostly of monoterpenoid or sesquiterpenoids. They are used as therapeutic agents in ethno, conventional, and complementary alternative medicines. Investigation and evaluation of the essential oil of Syzygium polyanthum as well as the antibacterial, antioxidant and antifungal activity was ascertained. The experiment was performed. 100 chemical constituents were obtained and two pure compound was isolated as Eugenol (1) and Farnesol (2). Significant growth inhibition of Staphylococcus aureus, (ATCCⓒ25923) Klebsiellia pneumonia (ATCCⓒ19155), Salmonella typhi (ATCCⓒ14028) and Escherichia coli (ATCC©25922) and the fungal strains Aspergillus flavin, Aspergillus niger, Candida, tropicalis, and Fusarium oxysporium was observed from the essential oil at concentration of 500 ㎍/mL. Antioxidant potential was observed to be strong of 18.42 ㎍/mL when compared to the control of 15.23 ㎍/mL. The result indicated that the oil obtained from root-bark of Syzygium polyanthum can be considered as an agent for antioxidant, antibacterial and antifungal in pharmaceutical food and cosmetic industries trails.

Keywords

References

  1. Aboh, M. I., Olayinka, B. O., Adeshina, G. O., & Oladosu, P. (2014). Preliminary studies on the antifungal activities of the phyto compounds from Mitracarpus villosus (Sw.) Dc aerial parts obtained from Abuja, Nigeria. Malaysian Journal of Microbiology, 10, 133-138.
  2. Adyani, K., Anwar, D.A., & Rohmawaty, E. (2018). Peningkatan Kadar Haemoglobin dengan Pemberian Ekstrak Daun Salam (Syzygium Polyanthum (Wight) Walp) pada Tikus Model Anaemia Defisiensi Besi. Majalah Kedokteran Bandung, 50(3), 167-172 https://doi.org/10.15395/mkb.v50n3.1390
  3. Alet, F. B., Assim, Z. B., bin Jusoh, I., & Ahmad, F. B. (2012). Chemical Constituents of Essential Oils from Resin and Bark of Agathis borneensis. Borneo Journal of Resource Science and Technology, 2(1), 28-32. https://doi.org/10.33736/bjrst.270.2012
  4. Amalina, A., MY, M., & Ashikin, A. K. (2013, March). Chemical composition, antioxidant and antibacterial activities of Syzygium polyanthum (Wight) Walp. essential oils. In The Open Conference Proceedings Journal (Vol. 4, No. 1).
  5. Awad, H. H., Ghazawy, N. A., & Rahman, K. A. (2013). Impact of farnesol on the food consumption and utilization, digestive enzymes and fat body proteins of the desert locust Schistocerca gregaria Forskal (Orthoptera: Acrididae). African entomology, 21(1), 126-132. https://doi.org/10.4001/003.021.0104
  6. Barboza, J.N., Filho, C.S.M., Silva,R.O., Medeiros, J.V.R., & Pergentino de Sousa, D. (2018). An Overview on the Anti-inflammatory Potential and Antioxidant Profile of Eugenol. Hindawi Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 3957262, 9 pages
  7. Bandara, H.MH.N., Herpin, M.J., Kolacny, D, Jr., Harb, A., Romanovicz, D., & Smyth, H.D.C. (2016). Incorporation of Fernesol Significantly Increases the Efficacy of Liposomal Ciprofloxacin against Pseudomonas aeruginosa Biofilms in vitro. Molecular Pharmaceutics 13(8), 2760-2770. https://doi.org/10.1021/acs.molpharmaceut.6b00360
  8. Boyan, B., James, H., & Judicael, P. (2005). Principles of accessing bacterial susceptibility to antibiotics using agar diffusion method, Journal of Antimicrobial Chemotherapy, 61, 1295-1301. https://doi.org/10.1093/jac/dkn090
  9. Bramston-Cook, R. (2010a). Kovats Indices for C2-C9 Hydrocarbons with Alumina PLOT Capillary Columns. Lotus Consulting.
  10. Bramston-Cook, R. (2010b). Kovats Indices for C2-C13 Hydrocarbons and Selected Oxygenates/Halocarbons with 100% Dimethylpolysiloxane Columns. Lotus Consulting.
  11. Costa, O.B.D., Del Menezzi, C. H. S., & Benedito, L. E. C. (2014). Essential oil constituents and yields from leaves of Blepharocalyx salicifolius (Kunt) O. Berg and Myracrodruon urundeuva (Allemao) collected during daytime. International Journal of Forestry Research, 2014, 6.
  12. Diba F., Yusro F., Mariani, Y., & Ohtani, K. (2013). Inventory and Biodiversity of Medicinal Plants from Tropical Rain Forest Based on Traditional Knowledge by Ethnic Dayaknese Communities in West Kalimantan Indonesia. Kuroshio Science, 7(1), 75-80.
  13. Fasihuddin, B.A., & Ibrahim, J. (2003). Chemical constituents of the essential oils of Goniothalamus uvariodes King. Flavour and Fragrance Journal, 18(2), 128-130. https://doi.org/10.1002/ffj.1142
  14. Fujisawa, S., Kadoma, Y., & Komoda, Y. (1988). 1H and 13C NMR studies of the interaction of eugenol, phenol, and triethyleneglycol dimethacrylate with phospholipid liposomes as a model system for odontoblast membranes. Journal of dental research, 67(11), 1438-1441. https://doi.org/10.1177/00220345880670111501
  15. Hidayati, M. D., Ersam, T., Shimizu, K., & Fatmawati, S. (2017). Antioxidant activity of Syzygium polyanthum extracts. Indonesian Journal of Chemistry, 17(1), 49-53. https://doi.org/10.22146/ijc.23545
  16. Ishag, K. E. A., Jork, H., & Zeppezauer, M. (1985). Mono-und Sesquiterpenalkoholglykoside. Fresenius' Zeitschrift fur analytische Chemie, 321(4), 331-336. https://doi.org/10.1007/BF00469377
  17. Ismail, A., & Ahmad, W. A. N. W. (2019). Syzygium polyanthum (Wight) Walp: A Potential phytomedicine. Pharmacognosy Journal, 11(2), 429-438). https://doi.org/10.5530/pj.2019.11.67
  18. Jabra-Razk, M.A., Meiller, C.E., & Shirtliff M.E. (2006). Effect of farnesol on Staphylococcus aureus biofilm formation and antimicrobial susceptibility. Antimicrobial Agents and Chemotherapy, 50(4), 1463-1469. https://doi.org/10.1128/AAC.50.4.1463-1469.2006
  19. Kamatou, G. P. Vermaak, I., and Viljoen, A. M. (2012). Eugenol-From the Remote Maluku Islands to the International. Molecules 17, 6953-6981. doi:10.3390/molecules17066953
  20. Prashanth, V. K., Chauhan, N. S., Padh, H., & Rajani, M. (2006). Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology, 107(2), 182-188. https://doi.org/10.1016/j.jep.2006.03.013
  21. McLaughlin, J. L., Rogers, L. L., & Anderson, J. E. (1998). The use of biological assays to evaluate botanicals. Drug Information Journal, 32(2), 513-524. https://doi.org/10.1177/009286159803200223
  22. Nova, Q. (2006). Organic Chemistry Select. Quim. Nova, 29(4), 11-14. https://doi.org/10.1590/S0100-40422006000100003
  23. Tailor, C.S., Goyal, A. (2014) Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. American Journal of Ethno medicine 1(4), 244-249.
  24. Ramage, G., Saville, S.P., Wickes, B.L., & Lopez-Ribot, J.L. (2002). Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule. Appl. Environ. Microbiol. 68(11), 5459-5463. https://doi.org/10.1128/AEM.68.11.5459-5463.2002
  25. Umaru, I.J., Badruddin, F. A., & Umaru, H.A. (2019). Phytochemical Screening of Essential Oils and Antibacterial Activity and Antioxidant Properties of Barringtonia asiatica (L) Leaf Extract. Hindawi Biochemistry Research International, 2019, ID 7143989, 6. https://doi.org/10.1155/2019/7143989
  26. Umaru, I.J., Samling, B., & Umaru, H.A. (2018). Phytochemical screening of Leucaena leucocephala leaf essential oil and its antibacterial potentials. MOJ Drug Design Development & Therapy, 2(6), 224-228.
  27. Umaru, I.J., Badruddin, F.A., Assim, Z.B., & Umaru, H.A. (2018). Cytotoxicity (Brine shrimp Lethality Bioassay) of Barringtonia racemosa Le aves, Stem-Bark and Root Extract. Journal of Biotechnology and Bioengineering, 2(2), 1-6.
  28. Zhang, H., Chen, X., & He, J.J. (2009). Pharmacological action of clove oil and its application in oral care products, Oral Care Industry. 19, 23-24.