대한본초학회지 (The Korea Journal of Herbology)

  • 제35권1호
  • /
  • Pages.9-18
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  • 2020
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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주사전자현미경을 이용한 3종 화분포자류(花粉胞子類) 한약재(송화분(松花粉), 포황(蒲黃), 해금사(海金沙))의 미세형태 비교연구

Comparative study on the external micro-morphology of 3 kinds of minute pollen and spore Herbs (Pini Pollen, Typhae Pollen, Lygodii Spora) utilizing scanning electron microscope

  • 김영식 (경희대학교 한의과대학 본초학교실) ;
  • 송준호 (한국한의학연구원 한약자원연구센터) ;
  • 최고야 (한국한의학연구원 한약자원연구센터) ;
  • 이금산 (원광대학교 한의과대학 본초학교실) ;
  • 주영승 (우석대학교 한의과대학 본초학교실)
  • Kim, Young-Sik (Department of Herbology, College of Korean Medicine, Kyunghee University) ;
  • Song, Jun-Ho (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Guemsan (Department of Herbology, College of Korean Medicine, Wonkwang University) ;
  • Ju, Young-Sung (Department of Herbology, College of Korean Medicine, Woosuk University)
  • 투고 : 2019.10.29
  • 심사 : 2020.01.25
  • 발행 : 2020.01.30
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초록

Objectives : We tried to establish standards for genuine by discriminating 3 kinds of pollen and spore herbs that are highly to be mixed with each other. Using an scanning electron microscope, we prepare a standard for discriminating external morphological characters of minute herbs, thereby contributing to the stable supply of genuine herbal medicines. Methods : Standard samples were confirmed by literature review on external morphological characteristics of original plants and herbal medicines, and collection and identification of original plants. The herbal medicines on the market were purchased and classified with using naked eye observation and magnifier. Finally, micromorphological identifications were conducted using an scanning electron microscope. Results : 1. Pini Pollen was clearly distinguished by its relatively medium size and a pollen grain with two swollen reticulate sacci at both ends. The verrucate ornamentation on the exine surface of the corpus and a sunken leptoma germ pore may be used as a discrimination criteria. 2. Typhae Pollen was distinguished by its relatively small size and a saccus on the end of a pollen grain. Reticulate ornamentation of exine surface of the corpus, and a slightly clear ulcerate germ pore can be used as a discrimination criteria. 3. Lygodii Spora was distinguished by its relatively large triangular-ovate shape and trichotomous fissure. Verrucate-tuberculate ornamentation of exine surface and trilete aperture could be used as a discrimination criteria. Conclusion : These results indicate that the use of electron microscopy is very effective for discriminating the external morphology of minute herbal medicines.

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