The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The protective effect of methanol extract of Corni Fructus on brain injury caused by unilateral common carotid artery occlusion in mice

산수유(山茱萸) 메탄올 추출물이 편측 경동맥 폐색으로 유도된 생쥐의 허혈성 뇌손상에 미치는 영향

  • Choi, Na Ri (Department of Korean Medicine, Pusan National University) ;
  • Jo, Sung Hyeon (Department of Korean Medicine, Pusan National University) ;
  • Lee, Se-Eun (Research Institute for Korean Medicine, Pusan National University) ;
  • Lee, Min Ji (Department of Korean Medical Science, Pusan National University) ;
  • Cho, Suin (Department of Korean Medicine, Pusan National University)
  • 최나리 (부산대학교 한의학전문대학원 한의학과) ;
  • 조성현 (부산대학교 한의학전문대학원 한의학과) ;
  • 이세은 (부산대학교 한의과학연구소) ;
  • 이민지 (부산대학교 한의학전문대학원 한의과학과) ;
  • 조수인 (부산대학교 한의학전문대학원 한의학과)
  • Received : 2019.10.24
  • Accepted : 2020.01.25
  • Published : 2020.01.30
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Abstract

Objectives : This study was conducted to evaluate the effects of Corni Fructus, the dried fruits of Cornus officinalis Sieb., on unilateral common carotid artery occlusion (UCCAO) in mouse model. Methods : The Corni Fructus used in the experiment was extracted with anhydrous methanol, then filtered and freeze-dried. C57BL/6 mice used in the experiments were conducted left UCCAO surgery to set up UCCAO rodent model for mice. The mice were divided into five groups for evaluate the effect of methanol extract of Corni Fructus (COM) on UCCAO induced ischemic brain injury. The expression levels of nitric oxide in cerebrum and serum, body weight change were measured. To determine the effect of UCCAO and COM administration on brain neurons, morphological changes of the cerebrum through a microscope was conducted. And western blot was performed to confirm the underlying mechanism of neuroprotective effect of COM administration. Results : COM administered UCCAO groups (CO50, CO150, and CO500) had no significant effects on nitric oxide production in ipsilateral hemisphere proteins and sera. The CO500, 500 mg/kg COM administration, attenuated UCCAO-induced p38 inflammatory signaling pathway and inflammatory mediators such as iNOS and COX-2. The CO500 group showed resilient morphological changes of hippocampus neuronal cells about brain damage caused by decreased flow of blood. These group also showed decreased inflammation and cellular stress response in neuronal cells. Conclusions : From these results, COM has a neuroprotective property via moderating inflammatory factors and cellular stress inducing factors in brain cells.

Keywords

References

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