The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Chemical Composition and Protective Effect of Essential Oils Derived from Medicinal Plant on PC12 Neuro-cells Induced by Oxidative Stress

약용식물 유래 정유성분 분석 및 산화 스트레스로부터 PC12 신경세포 보호 효과

  • Lee, Ji Yeon (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Park, Jeong-Yong (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Dong Hwi (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Su Ji (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Gwi Young (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Seo, Kyung Hye (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
  • 이지연 (충북대학교 식품공학과) ;
  • 박정용 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최수지 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 장귀영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 서경혜 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2020.03.30
  • Accepted : 2020.04.10
  • Published : 2020.04.30
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Abstract

The purpose of this study was to investigate the protective effect on oxidative stress induced PC12 cells, and volatile flavor composition of essential oils derived from medicinal plant seeds- Gossypium hirsutum L. (G. hirsutum), Coix lachryma-jobi (C. lachryma-jobi) and Oenothera biennis (O. biennis). The essential oils were obtained by the solvent (hexane) extraction method from the seeds. The essential oils of the seeds were analyzed by the solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). The major compounds of G. hirsutum, C. lachryma-jobi and O. biennis were cyclonexanol (16.65%), β-asarone (14.29%) and ylangene (50.01%). The DPPH radical scavenging activity (IC50) was the highest value of 8.52 mg/mL in the O. biennis. Additionally, IC50 values of G. hirsutum and C. lachryma-jobi were 26.76 mg/mL and 36.81 mg/mL. For the oxidative stress on PC12 cells, we treated with hydrogen peroxide (H2O2). The pretreatment of oxidative stress induced PC12 cells with all the essential oils preserved or increased their cell viability and G. hirsutum and O. biennis attenuated the ROS generation (by 68.75% and 56.25% vs. H2O2 control). The results of this study suggest that the essential oils derived from medicinal plant seeds could be used as valuable back data as a natural essential oil material to prevent neurodegenerative diseases by protecting neuro-cells.

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References

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