Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Synthesis of Anabolic Steroid Glycosides by Glucosyltransferase from Terribacillus sp. PAMC 23288

  • Yu, Eun-Ji (Department of Life Science and Biochemical Engineering, SunMoon University) ;
  • Yamaguchi, Tokutaro (Department of Life Science and Biochemical Engineering, SunMoon University) ;
  • Lee, Joo-Ho (Genome-Based BioIT Convergence Institute) ;
  • Lim, A-Rang (Korea Institute of Oriental Medicine) ;
  • Lee, Jun Hyuck (Unit of Research for Practical Application, Korea Polar Research Institute) ;
  • Park, Hyun (Division of Biotechnology, College of Life Science and Biotechnology, Korea University) ;
  • Oh, Tae-Jin (Department of Life Science and Biochemical Engineering, SunMoon University)
  • Received : 2019.11.26
  • Accepted : 2019.12.24
  • Published : 2020.04.28
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Abstract

The application of steroids has steadily increased thanks to their therapeutic effects. However, alternatives are required due their severe side effects; thus, studies on the activities of steroid derivatives are underway. Sugar derivatives of nandrolone, which is used to treat breast cancer, as well as cortisone and prednisone, which reduce inflammation, pain, and edema, are unknown. We linked O-glucose to nandrolone and testosterone using UDP-glucosyltransferase (UGT-1) and, then, tested their bioactivities in vitro. Analysis by NMR showed that the derivatives were 17β-nandrolone β-ᴅ-glucose and 17β-testosterone β-ᴅ-glucose, respectively. The viability was higher and cytotoxicity was evident in PC12 cells incubated with rotenone and, testosterone derivatives, compared to the controls. SH-SY5Y cells incubated with H2O2 and nandrolone derivatives remained viable and cytotoxicity was attenuated. Both derivatives enhanced neuronal protective effects and increased the amounts of cellular ATP.

Keywords

References

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