Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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α-Glucosidase Inhibitory Activity of the Ethanol Extract of Peanut (Arachis hypogaea L.) Skin

땅콩 속껍질 에탄올 추출물의 알파-글루코시데이즈 억제활성

  • 하태정 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 이명희 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 오은영 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 김정인 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 송석보 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 곽도연 (농촌진흥청 국립식량과학원 밭작물개발과)
  • Received : 2019.10.31
  • Accepted : 2019.12.16
  • Published : 2020.02.28
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Abstract

Background: Owing to its high efficiency in lipid and protein production, peanut (Arachis hypogaea L.) is considered one of most important crops world-wide. The kernels of peanuts are undoubtedly the most important product this plant, whereas the skin is almost completely neglected in nutraceutical terms. However, peanut skin contains potentially health-promoting phenolics and dietary fiber, and there is considerable potential for commercial exploitation. In this study, we evaluated the α-glucosidase inhibitory activity of an extract of peanut skin (PS). Methods and Results: The α-glucosidase inhibitory effects of 80% ethanol extracts of peanut (A. hypogaea L. 'Sinpalkwang') skin were evaluated and found to have a half-maximal inhibitory concentration (IC50) value of 1.2 ㎍/㎖. Progress curves for enzyme reactions were recorded spectrophotometrically, and the inhibition kinetics revealed time-dependent inhibition with enzyme isomerization. Furthermore, using ultra-high performance liquid chromatography combined with quadrupole-orbitrap mass spectrometry, we identified 26 compounds in the peanut skin extract, namely, catechin, epicatechin, and 24 proanthocyanidins. Conclusions: The results suggest that peanut skin can be utilized as an effective source of α-glucosidase inhibition in functional foods and nutraceuticals.

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References

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