Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Transcriptome analysis of Panax ginseng response to high light stress

  • Jung, Je Hyeong (Center for Natural Products Convergence Research, Korea Institute of Science and Technology (KIST)) ;
  • Kim, Ho-Youn (Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST)) ;
  • Kim, Hyoung Seok (Center for Natural Products Convergence Research, Korea Institute of Science and Technology (KIST)) ;
  • Jung, Sang Hoon (Center for Natural Products Convergence Research, Korea Institute of Science and Technology (KIST))
  • Received : 2018.03.06
  • Accepted : 2018.12.24
  • Published : 2020.03.15
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Abstract

Background: Ginseng (Panax ginseng Meyer) is an essential source of pharmaceuticals and functional foods. Ginseng productivity has been compromised by high light (HL) stress, which is one of the major abiotic stresses during the ginseng cultivation period. The genetic improvement for HL tolerance in ginseng could be facilitated by analyzing its genetic and molecular characteristics associated with HL stress. Methods: Genome-wide analysis of gene expression was performed under HL and recovery conditions in 1-year-old Korean ginseng (P. ginseng cv. Chunpoong) using the Illumina HiSeq platform. After de novo assembly of transcripts, we performed expression profiling and identified differentially expressed genes (DEGs). Furthermore, putative functions of identified DEGs were explored using Gene Ontology terms and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. Results: A total of 438 highly expressed DEGs in response to HL stress were identified and selected from 29,184 representative transcripts. Among the DEGs, 326 and 114 transcripts were upregulated and downregulated, respectively. Based on the functional analysis, most upregulated and a significant number of downregulated transcripts were related to stress responses and cellular metabolic processes, respectively. Conclusion: Transcriptome profiling could be a strategy to comprehensively elucidate the genetic and molecular mechanisms of HL tolerance and susceptibility. This study would provide a foundation for developing breeding and metabolic engineering strategies to improve the environmental stress tolerance of ginseng.

Keywords

References

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