Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Dewormer drug fenbendazole has antiviral effects on BoHV-1 productive infection in cell cultures

  • Chang, Long (College of Veterinary Medicine, Yangzhou University) ;
  • Zhu, Liqian (College of Veterinary Medicine, Yangzhou University)
  • Received : 2020.04.27
  • Accepted : 2020.07.21
  • Published : 2020.09.30
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Abstract

Background: Fenbendazole, a dewormer drug, is used widely in the clinical treatment of parasite infections in animals. Recent studies have shown that fenbendazole has substantial effects on tumor growth, immune responses, and inflammatory responses, suggesting that fenbendazole is a pluripotent drug. Nevertheless, the antiviral effects have not been reported. Fenbendazole can disrupt microtubules, which are essential for multiple viruses infections, suggesting that fenbendazole might have antiviral effects. Objectives: This study examined whether fenbendazole could inhibit bovine herpesvirus 1 (BoHV-1) productive infection in cell cultures. Methods: The effects of fenbendazole on viral production, transcription of the immediate early (IE) genes, viron-associated protein expression, and the cellular signaling PLC-γ1/Akt pathway were assessed using distinct methods. Results: Fenbendazole could inhibit BoHV-1 productive infections significantly in MDBK cells in a dose-dependent manner. A time-of-addition assay indicated that fenbendazole affected both the early and late stages in the virus replication cycles. The transcription of IE genes, including BoHV-1 infected cell protein 0 (bICP0), bICP4, and bICP22, as well as the synthesis of viron-associated proteins, were disrupted differentially by the fenbendazole treatment. The treatment did not affect the cellular signaling pathway of PLC-γ1/Akt, a known cascade playing important roles in virus infection. Conclusions: Overall, fenbendazole has antiviral effects on BoHV-1 replication.

Keywords

Acknowledgement

This research was supported by Chinese National Science Foundation Grant(Grant Nos. 31772743 and 31972655), and partially supported by A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Key Laboratory for Preventive Research of Emerging Animal Diseases in Foshan University (KLPREAD201801-14).

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