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Epidemiological investigation of porcine pseudorabies virus and its coinfection rate in Shandong Province in China from 2015 to 2018

  • Ma, Zicheng (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Han, Zifeng (Emergency Centre for the Control of Transboundary Animal Diseases, Food and Agriculture Organization of the United Nations) ;
  • Liu, Zhaohu (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Meng, Fanliang (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Wang, Hongyu (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Cao, Longlong (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Li, Yan (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Jiao, Qiulin (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Liu, Sidang (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Liu, Mengda (College of Animal Science and Technology, Shandong Agricultural University)
  • 투고 : 2019.07.23
  • 심사 : 2020.01.26
  • 발행 : 2020.05.31

초록

Background: Pseudorabies, also known as Aujeszky's disease, is caused by the pseudorabies virus (PRV) and has been recognized as a critical disease affecting the pig industry and a wide range of animals around the world, resulting in great economic losses each year. Shandong province, one of the most vital food animal-breeding regions in China, has a very dense pig population, within which pseudorabies infections were detected in recent years. The data, however, on PRV epidemiology and coinfection rates of PRV with other major swine diseases is sparse. Objectives: This study aimed to investigate the PRV epidemiology in Shandong and analyze the current control measures. Methods: In this study, a total number of 16,457 serum samples and 1,638 tissue samples, which were collected from 362 intensive pig farms (≥ 300 sows/farm) covered all cities in Shandong, were tested by performing enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results: Overall, 52.7% and 91.5% of the serum samples were positive for PRV-gE and -gB, respectively, based on ELISA results. In addition, 15.7% of the tissue samples were PCR positive for PRV. The coinfection rates of PRV with porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus, and classical swine fever virus were measured; coinfection with PCV2 was 35.0%, higher than those of the other two viruses. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Conclusions: The results demonstrate the PRV prevalence and its coinfection rates in Shandong province and indicate that pseudorabies is endemic in pig farms in this region. This study provides epidemiological data that can be useful in the prevention and control of pseudorabies in Shandong, China.

키워드

과제정보

This study is supported from Shandong "Double Tops" Program, National Key Research and Development Program of China, Grant/Award Number: 2017YFD0500600.

참고문헌

  1. Mettenleiter TC. Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis--state of the art, June 1999. Vet Res. 2000;31(1):99-115.
  2. Pomeranz LE, Reynolds AE, Hengartner CJ. Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev. 2005;69(3):462-500. https://doi.org/10.1128/MMBR.69.3.462-500.2005
  3. Crandell RA. Pseudorabies (Aujeszky's disease). Vet Clin North Am Large Anim Pract. 1982;4(2):321-331. https://doi.org/10.1016/S0196-9846(17)30108-8
  4. Rziha HJ, Mettenleiter TC, Ohlinger V, Wittmann G. Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissues. Virology. 1986;155(2):600-613. https://doi.org/10.1016/0042-6822(86)90220-5
  5. Muller T, Hahn EC, Tottewitz F, Kramer M, Klupp BG, Mettenleiter TC, Freuling C. Pseudorabies virus in wild swine: a global perspective. Arch Virol. 2011;156(10):1691-1705. https://doi.org/10.1007/s00705-011-1080-2
  6. Smith G. Preferential sexual transmission of pseudorabies virus in feral swine populations may not account for observed seroprevalence in the USA. Prev Vet Med. 2012;103(2-3):145-156. https://doi.org/10.1016/j.prevetmed.2011.09.010
  7. Tong W, Li G, Liang C, Liu F, Tian Q, Cao Y, Li L, Zheng X, Zheng H, Tong G. A live, attenuated pseudorabies virus strain JS-2012 deleted for gE/gI protects against both classical and emerging strains. Antiviral Res. 2016;130:110-117. https://doi.org/10.1016/j.antiviral.2016.03.002
  8. An TQ, Peng JM, Tian ZJ, Zhao HY, Li N, Liu YM, Chen JZ, Leng CL, Sun Y, Chang D, Tong GZ. Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012. Emerg Infect Dis. 2013;19(11):1749-1755. https://doi.org/10.3201/eid1911.130177
  9. Wu R, Bai C, Sun J, Chang S, Zhang X. Emergence of virulent pseudorabies virus infection in northern China. J Vet Sci. 2013;14(3):363-365. https://doi.org/10.4142/jvs.2013.14.3.363
  10. Wang Y, Qiao S, Li X, Xie W, Guo J, Li Q, Liu X, Hou J, Xu Y, Wang L, Guo C, Zhang G. Molecular epidemiology of outbreak-associated pseudorabies virus (PRV) strains in central China. Virus Genes. 2015;50(3):401-409. https://doi.org/10.1007/s11262-015-1190-0
  11. Song C, Gao L, Bai W, Zha X, Yin G, Shu X. Molecular epidemiology of pseudorabies virus in Yunnan and the sequence analysis of its gD gene. Virus Genes. 2017;53(3):392-399. https://doi.org/10.1007/s11262-017-1429-z
  12. Xia L, Sun Q, Wang J, Chen Q, Liu P, Shen C, Sun J, Tu Y, Shen S, Zhu J, Zhao H, Wang Q, Li B, Tao J, Soares Magalhaes RJ, Yan Y, Cai C. Epidemiology of pseudorabies in intensive pig farms in Shanghai, China: Herd-level prevalence and risk factors. Prev Vet Med. 2018;159:51-56. https://doi.org/10.1016/j.prevetmed.2018.08.013
  13. Sun Y, Luo Y, Wang CH, Yuan J, Li N, Song K, Qiu HJ. Control of swine pseudorabies in China: opportunities and limitations. Vet Microbiol. 2016;183:119-124. https://doi.org/10.1016/j.vetmic.2015.12.008
  14. Hu D, Lv L, Zhang Z, Xiao Y, Liu S. Seroprevalence and associated risk factors of pseudorabies in Shandong province of China. J Vet Sci. 2016;17(3):361-368. https://doi.org/10.4142/jvs.2016.17.3.361
  15. Gu J, Hu D, Peng T, Wang Y, Ma Z, Liu Z, Meng F, Shang Y, Liu S, Xiao Y. Epidemiological investigation of pseudorabies in Shandong Province from 2013 to 2016. Transbound Emerg Dis. 2018;65(3):890-898. https://doi.org/10.1111/tbed.12827
  16. Sabo A. Analysis of reactivation of latent pseudorabies virus infection in tonsils and Gasserian ganglia of pigs. Acta Virol. 1985;29(5):393-402.
  17. Cheng Z, Kong Z, Liu P, Fu Z, Zhang J, Liu M, Shang Y. Natural infection of a variant pseudorabies virus leads to bovine death in China. Transbound Emerg Dis. Forthcoming 2020;67(2):518-522.
  18. Liu Y, Zhang S, Xu Q, Wu J, Zhai X, Li S, Wang J, Ni J, Yuan L, Song X, Zhao B, Zhou Z, Wang C, Yang L. Investigation on pseudorabies prevalence in Chinese swine breeding farms in 2013-2016. Trop Anim Health Prod. 2018;50(6):1279-1285. https://doi.org/10.1007/s11250-018-1555-1
  19. Liu M, Kemper N, Volkmann N, Schulz J. Resistance of enterococcus spp. in dust from farm animal houses: a retrospective study. Front Microbiol. 2018;9:3074. https://doi.org/10.3389/fmicb.2018.03074
  20. Mak CK, Yang C, Jeng CR, Pang VF, Yeh KS. Reproductive failure associated with coinfection of porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus. Can Vet J. 2018;59(5):525-530.
  21. Dias AS, Gerber PF, Araujo AS, Auler PA, Gallinari GC, Lobato ZI. Lack of antibody protection against Porcine circovirus 2 and Porcine parvovirus in naturally infected dams and their offspring. Res Vet Sci. 2013;94(2):341-345. https://doi.org/10.1016/j.rvsc.2012.09.009