Journal of Veterinary Science

  • 제21권5호
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  • Pages.77.1-77.14
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Analysis of virulence traits of Staphylococcus aureus isolated from bovine mastitis in semi-intensive and family dairy farms

  • Guzman-Rodriguez, Jaquelina J. (Life Science Division, Postgraduate Program in Bioscience, Irapuato-Salamanca Campus, University of Guanajuato) ;
  • Leon-Galvan, Ma. Fabiola (Life Science Division, Postgraduate Program in Bioscience, Irapuato-Salamanca Campus, University of Guanajuato) ;
  • Barboza-Corona, Jose E. (Life Science Division, Postgraduate Program in Bioscience, Irapuato-Salamanca Campus, University of Guanajuato) ;
  • Valencia-Posadas, Mauricio (Life Science Division, Postgraduate Program in Bioscience, Irapuato-Salamanca Campus, University of Guanajuato) ;
  • Loeza-Lara, Pedro D. (Department of Food Genomics, University of La Cienega of the State of Michoacan de Ocampo) ;
  • Sanchez-Ceja, Monica (Department of Food Genomics, University of La Cienega of the State of Michoacan de Ocampo) ;
  • Ochoa-Zarzosa, Alejandra (Multidisciplinary Center for Biotechnology Studies, Faculty of Veterinary Medicine and Zootechnics, Universidad Michoacana de San Nicolas de Hidalgo) ;
  • Lopez-Meza, Joel E. (Multidisciplinary Center for Biotechnology Studies, Faculty of Veterinary Medicine and Zootechnics, Universidad Michoacana de San Nicolas de Hidalgo) ;
  • Gutierrez-Chavez, Abner J. (Life Science Division, Postgraduate Program in Bioscience, Irapuato-Salamanca Campus, University of Guanajuato)
  • 투고 : 2020.04.08
  • 심사 : 2020.08.11
  • 발행 : 2020.09.30
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초록

Background: Staphylococcus aureus is one of the main microorganisms that causes bovine mastitis, and its well-known virulence characteristics and interactions with the environment are used to aid the design of more efficient therapies. Objectives: To determine whether the virulence traits, such as antibiotic resistance and biofilm-forming and internalization abilities, of S. aureus isolated from bovine mastitis are related to dairy production system types. Methods: The study was performed in the Mexican states of Guanajuato and Michoacan. Semi-intensive dairy farms (SIDFs) and family dairy farms (FDFs) (454 and 363 cows, respectively) were included. The 194 milk samples from mastitis affected quarters were collected and 92 strains of S. aureus were isolated and identified by biochemical and molecular tests. Antibiotic resistance, biofilm and internalization assays were performed on 30 randomly selected isolated strains to determine virulence traits, and these strains were equally allocated to the 2 dairy production systems. Results: All 30 selected strains displayed a high degree of resistance (50%-91.7%) to the antibiotics tested, but no significant difference was found between SIDF and FDF isolates. S. aureus strains from SIDFs had an average biofilm forming capacity of up to 36% (18.9%-53.1%), while S. aureus strains from FDFs registered an average of up to 53% (31.5%-77.8%) (p > 0.05). Internalization assays revealed a higher frequency of internalization capacity for strains isolated from FDFs (33.3%) than for those isolated from SIDFs (6.7%) (p > 0.05). fnbpA gen was detected in 46.6% of FDF strains and 33.3% of SIDF strains, and this difference was significant (p < 0.05). Conclusions: Our findings show that the virulence traits of S. aureus isolates analyzed in this study, depend significantly on several factors, such as phenotype, genotype, and environmental conditions, which are significantly related to dairy production system type and daily management practices.

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과제정보

The authors thank students and colleagues at University of Guanajuato for their technical support and express their appreciation to the dairy producers for the contributions they made during this study.

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