Journal of Veterinary Science

  • 제21권6호
  • /
  • Pages.90.1-90.11
  • /
  • 2020
  • /
  • 1229-845X(pISSN)
  • /
  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

Can bone marrow aspirate improve mandibular fracture repair in camels (Camelus dromedarius)? A preliminary study

  • Al-Sobayil, Fahd (Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University) ;
  • Sadan, Madeh A. (Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University) ;
  • El-Shafaey, El-Sayed (Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University) ;
  • Ahmed, Ahmed F. (Department of Animal Surgery, Faculty of Veterinary Medicine, Assiut University)
  • 투고 : 2019.12.05
  • 심사 : 2020.10.08
  • 발행 : 2020.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Mandibular fractures are common in camels, leading to considerable economic losses. This study explored methods of improving mandibular fractures repair, adjuvant with interdental wire, or bone plate fixation. Autologous bone marrow (BM) injection enhances osteogenesis and rapid healing. Objectives: To investigate the effect of autologous BM aspirate as an adjuvant treatment for repairing mandibular fractures in camels with interdental wire, or bone plate fixation. Methods: Thirty dromedary camels aged 5-8 years and of both sexes were randomly divided into 4 treatment groups: group 1 (n = 10) treated with stainless steel wire fixation and BM injection at the fracture line, group 2 (n = 10) treated with plate fixation and BM injection at the fracture line, group 3 (n = 5) treated with stainless steel bone wire fixation and placebo saline injection at the fracture line, and group 4 (n = 5) treated with plate fixation and placebo injection at the fracture line. The mandibular fractures were followed weekly for 12 weeks postoperatively to assess improvement and healing based on clinical evaluation, radiographic union scale, and bone turnover markers (i.e., bone alkaline phosphatase, osteocalcin, pyridinoline, and deoxypyridinoline). Results: Compared to other groups, elevated bone turnover markers in group 1 were demonstrated (p < 0.05) on the seventh postoperative day. Likewise, compared to other groups, both clinical findings and radiographic union scale significantly improved (p < 0.05) in group 1 on the 56th postoperative day. Conclusions: BM aspirate has a promising beneficial osteogenic effect on mandibular fracture repair in camels, most notably when combined with interdental wire fixation.

키워드

과제정보

The authors wish to thank Prof Dr Sabry El-Khodary for his help in performing the statistical analysis of this article.

참고문헌

  1. Siddiqui MI, Telfah MN, Rashid J, Taleb SA. Modified interdental wiring technique for mandibular fractures in camels: a clinical study. J Vet Anim Sci. 2012;2:57-60.
  2. Siddiqui MI, Anwar S, Telfah MN. Management of atypical mandibular fractures by cerclage and standard interdental wiring technique in the dromedary camel (a report of four cases). J Camel Pract Res. 2012;19(2):177-181.
  3. Ahmed AF. Mandibular fracture in single-humped camels. Vet Surg. 2011.40(7):903-907. https://doi.org/10.1111/j.1532-950X.2011.00898.x
  4. Ramadan RO. Incidence, classification and treatment of 179 fractures in camels (Camelus dromedarius). In: Proceedings of the First International Camel Conference; February 6, 1992, Dubai, United Arab Emirates, 347-351.
  5. Gahlot TK, Chouhan DS. Fractures in dromedary (Camelus dromedarius) - a retrospective study. J Camel Pract Res. 1994;1:9-14.
  6. Belsito KA, Fischer AT. External skeletal fixation in the management of equine mandibular fractures: 16 cases (1988-1998). Equine Vet J. 2001;33(2):176-183. https://doi.org/10.1111/j.2042-3306.2001.tb00597.x
  7. Cetinkaya M, Demirutku A. Interfragmental fixation of rostral mandibular fracture with cerclage wire in a thoroughbred English horse. Turk J Vet Anim Sci. 2012;36:67-71.
  8. Ramzan PL. Management of rostral mandibular fractures in the young horse. Equ Vet Edu. 2008;20(2):107-112. https://doi.org/10.2746/095777308X272094
  9. Hanuman R, Gahlot TK, Ram H. Gross and radiological evaluation of RBR IDW technique for repair of mandibular fracture in camels (Camelus dromedarius). J Camel Pract Res. 2001;8(2):199-202.
  10. Karrouf G, Rizk A, El-Shafaey E, Abouelnasr K, Abou-Alsaud M, Gomaa M, et al. Management of bilateral mandibular fractures using intraoral unilateral application of bone plates in the dromedary camel. ARC J Anim Vet Sci. 2017;3(1):8-12.
  11. Lavania JP. A field oriented immobilization technique for mandibular fracture in camel: a clinical study. In: Proceedings of the 3rd Annual Meeting for Animal Production under Arid Conditions; May 2-3 1998, Al-Ain, United Arab Emirates, 174-179.
  12. Purohit RK, Dubi PR, Choudhary RJ. Amputation of anterior fragment of the irreparable mandibular fracture in camel (Camelus dromedarius): a report of five cases. Indian Vet J. 1984;61:989-991.
  13. Bianco P, Riminucci M, Gronthos S, Robey PG. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells. 2001;19(3):180-192. https://doi.org/10.1634/stemcells.19-3-180
  14. Veronesi F, Giavaresi G, Tschon M, Borsari V, Nicoli Aldini N, Fini M. Clinical use of bone marrow, bone marrow concentrate, and expanded bone marrow mesenchymal stem cells in cartilage disease. Stem Cells Dev. 2013;22(2):181-192. https://doi.org/10.1089/scd.2012.0373
  15. Vilquin JT, Rosset P. Mesenchymal stem cells in bone and cartilage repair: current status. Regen Med. 2006;1(4):589-604. https://doi.org/10.2217/17460751.1.4.589
  16. Antariksa MI. Bone healing in femoral fracture of white rat with intramedullary wire fixation and additional medullary bone marrow. J Indones Orthop. 2012;40(2):13-16.
  17. Hatzokos I, Stavridis SI, Iosifidou E, Karataglis D, Christodoulou A. Autologous bone marrow grafting combined with demineralized bone matrix improves consolidation of docking site after distraction osteogenesis. J Bone Joint Surg Am. 2011;93(7):671-678. https://doi.org/10.2106/JBJS.J.00514
  18. Kassem MS. Percutaneous autogenous bone marrow injection for delayed union or non union of fractures after internal fixation. Acta Orthop Belg 2013.79(6):711-717.
  19. Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N. Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones. Bone. 2007;40(2):522-528. https://doi.org/10.1016/j.bone.2006.09.019
  20. Allen MJ. Biochemical markers of bone metabolism in animals: uses and limitations. Vet Clin Pathol. 2003;32(3):101-113. https://doi.org/10.1111/j.1939-165X.2003.tb00323.x
  21. Al-Sobayil FA. Influence of exercise on bone formation and bone resorption biomarkers in sera of racing dromedary camels. J Camel Pract Res. 2008;15(1):43-48.
  22. Al-Sobayil FA. Circadian rhythm of bone formation biomarkers in serum of dromedary camels. Res Vet Sci. 2010;89(3):455-459. https://doi.org/10.1016/j.rvsc.2010.03.024
  23. El-Shafaey ES, Hamed M, Abdellatif A, Abo Elfadl E. Comparison of blind, ultrasound and computed tomographic-guided injection techniques for nerve block of the head in one-humped camel (Camelus dromedaries) cadavers. Pak Vet J. 2017;37(2):180-184.
  24. Mitchell CF, Richbourg HA, Goupil BA, Gillett AN, McNulty MA. Assessment of tuber coxae bone biopsy in the standing horse. Vet Surg. 2017;46(3):396-402. https://doi.org/10.1111/vsu.12603
  25. Caplan AI, Dennis JE. Mesenchymal stem cells as trophic mediators. J Cell Biochem. 2006;98(5):1076-1084. https://doi.org/10.1002/jcb.20886
  26. Chapman MW. Closed intra medullary nailing of femoral shaft fractures: technique and rationale. Contemp Orthop. 1982;4:2-13.
  27. Hernigou P, Poignard A, Beaujean F, Rouard H. Percutaneous autologous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am. 2005;87(7):1430-1437.
  28. Nuss K, Kostlin R, Elma E, Matis U. Mandibular fractures in cattle--treatment and results. Tierarztl Prax. 1991;19(1):27-33.
  29. Coulibaly MO, Sietsema DL, Burgers TA, Mason J, Williams BO, Jones CB. Recent advances in the use of serological bone formation markers to monitor callus development and fracture healing. Crit Rev Eukaryot Gene Expr. 2010;20(2):105-127. https://doi.org/10.1615/CritRevEukarGeneExpr.v20.i2.20
  30. Cox G, Einhorn TA, Tzioupis C, Giannoudis PV. Bone-turnover markers in fracture healing. J Bone Joint Surg Br. 2010;92(3):329-334.
  31. El-Shafaey EA, Aoki T, Ishii M, Yamada K. Conservative management with external coaptation technique for treatment of a severely comminuted fracture of the proximal phalanx in a Holstein-Friesian cow. Majallah-i Tahqiqat-i Dampizishki-i Iran. 2014;15:300-303.
  32. Ali AM, El-Alfy B, Amin M, Nematalla M, El-Shafaey SA. Can platelet-rich plasma shorten the consolidation phase of distraction osteogenesis? An experimental study. Eur J Orthop Surg Traumatol. 2015;25(3):543-548. https://doi.org/10.1007/s00590-014-1542-9
  33. Sousa CP, Lopez-Pena M, Guzon FM, Abreu HV, Luis MR, Viegas CA, et al. Evaluation of bone turnover markers and serum minerals variations for predicting fracture healing versus non-union processes in adult sheep as a model for orthopedic research. Injury. 2017;48(8):1768-1775. https://doi.org/10.1016/j.injury.2017.05.025