Journal of Veterinary Science

  • 제21권5호
  • /
  • Pages.71.1-71.10
  • /
  • 2020
  • /
  • 1229-845X(pISSN)
  • /
  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

Development of a novel reverse transcription PCR and its application to field sample testing for feline calicivirus prevalence in healthy stray cats in Korea

  • Kim, Sung Jae (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Yong Ho (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Kun Taek (Department of Biotechnology, Inje University)
  • 투고 : 2020.03.10
  • 심사 : 2020.08.03
  • 발행 : 2020.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Feline calicivirus (FCV) is a major and highly infectious pathogen in cats worldwide. However, there have been limited studies about the status of FCV infections in Korea. Objectives: To investigate the current status of FCV infections in stray cats in Korea. Methods: A novel reverse transcription polymerase chain reaction (RT-PCR) assay was developed based on the conserved nucleotide sequences of reported FCV strains. Field swab samples were collected from 122 cats (2 hospital admitted cats and 120 stray cats) in 2016 and 2017. All the samples were tested by virus isolation and 2 different RT-PCRs, including the novel RT-PCR, for the detection of FCV. Results: The novel RT-PCR assay showed no cross-reactivity to the nucleic acids of the other feline pathogens tested, and the limit of detection was calculated as 100 TCID50/mL based on an in vitro assessment. The novel RT-PCR assay detected 5 positive samples from the 122 field samples, which showed perfect agreement with the results of the virus isolation method. In contrast, another RT-PCR assay used in a previous study in Korea detected no positive samples. The prevalence of FCV infection in stray cats was 2.5% (3/120) based on the results of virus isolation and the novel RT-PCR assays. Conclusions: The current study is the first report of the detection and prevalence of FCV in stray cats in Korea. The novel RT-PCR assay developed in this study showed high sensitivity and specificity, which indicates a useful diagnostic assay to identify FCV infection in cats.

키워드

과제정보

This work was supported by the 2019 Inje University Research Grant.

참고문헌

  1. Cai Y, Fukushi H, Koyasu S, Kuroda E, Yamaguchi T, Hirai K. An etiological investigation of domestic cats with conjunctivitis and upper respiratory tract disease in Japan. J Vet Med Sci. 2002;64(3):215-219. https://doi.org/10.1292/jvms.64.215
  2. Hurley KE, Pesavento PA, Pedersen NC, Poland AM, Wilson E, Foley JE. An outbreak of virulent systemic feline calicivirus disease. J Am Vet Med Assoc. 2004;224(2):241-249. https://doi.org/10.2460/javma.2004.224.241
  3. Hurley KF, Sykes JE. Update on feline calicivirus: new trends. Vet Clin North Am Small Anim Pract. 2003;33(4):759-772. https://doi.org/10.1016/S0195-5616(03)00025-1
  4. Marsilio F, Di Martino B, Decaro N, Buonavoglia C. A novel nested PCR for the diagnosis of calicivirus infections in the cat. Vet Microbiol. 2005;105(1):1-7. https://doi.org/10.1016/j.vetmic.2004.09.017
  5. Sykes JE, Allen JL, Studdert VP, Browning GF. Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR. Vet Microbiol. 2001;81(2):95-108. https://doi.org/10.1016/S0378-1135(01)00340-6
  6. Murphy FA, Gibbs EP, Horzinek MC, Studdert MJ. Chapter 12: Laboratory diagnosis of viral diseases. In: Veterinary Virology. 3rd ed. Cambridge: Academic Press; 1999, 193-224.
  7. Radford AD, Addie D, Belak S, Boucraut-Baralon C, Egberink H, Frymus T, et al. Feline calicivirus infection. ABCD guidelines on prevention and management. J Feline Med Surg. 2009;11(7):556-564. https://doi.org/10.1016/j.jfms.2009.05.004
  8. Gaskell RM, Dennis PE, Goddard LE, Cocker FM, Wills JM. Isolation of felid herpesvirus I from the trigeminal ganglia of latently infected cats. J Gen Virol. 1985;66(Pt 2):391-394. https://doi.org/10.1099/0022-1317-66-2-391
  9. Berger A, Willi B, Meli ML, Boretti FS, Hartnack S, Dreyfus A, et al. Feline calicivirus and other respiratory pathogens in cats with feline calicivirus-related symptoms and in clinically healthy cats in Switzerland. BMC Vet Res. 2015;11:282. https://doi.org/10.1186/s12917-015-0595-2
  10. Chao L. Fitness of RNA virus decreased by Muller's ratchet. Nature. 1990;348(6300):454-455. https://doi.org/10.1038/348454a0
  11. Sykes JE, Studdert VP, Browning GF. Detection and strain differentiation of feline calicivirus in conjunctival swabs by RT-PCR of the hypervariable region of the capsid protein gene. Arch Virol. 1998;143(7):1321-1334. https://doi.org/10.1007/s007050050378
  12. Ohe K, Sakai S, Sunaga F, Murakami M, Kiuchi A, Fukuyama M, et al. Detection of feline calicivirus (FCV) from vaccinated cats and phylogenetic analysis of its capsid genes. Vet Res Commun. 2006;30(3):293-305. https://doi.org/10.1007/s11259-006-3232-5
  13. Griffin HG, Griffin AM. DNA sequencing. Recent innovations and future trends. Appl Biochem Biotechnol. 1993;38(1-2):147-159. https://doi.org/10.1007/BF02916418
  14. Seal BS. Analysis of capsid protein gene variation among divergent isolates of feline calicivirus. Virus Res. 1994;33(1):39-53. https://doi.org/10.1016/0168-1702(94)90016-7
  15. Coyne KP, Dawson S, Radford AD, Cripps PJ, Porter CJ, McCracken CM, et al. Long-term analysis of feline calicivirus prevalence and viral shedding patterns in naturally infected colonies of domestic cats. Vet Microbiol. 2006;118(1-2):12-25. https://doi.org/10.1016/j.vetmic.2006.06.026
  16. Kang BT, Park HM. Prevalence of feline herpesvirus 1, feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter. J Vet Sci. 2008;9(2):207-209. https://doi.org/10.4142/jvs.2008.9.2.207
  17. Smeeton NC. Early history of the kappa statistic. Biometrics. 1985;41(3):795.
  18. Radford AD, Coyne KP, Dawson S, Porter CJ, Gaskell RM. Feline calicivirus. Vet Res. 2007;38(2):319-335. https://doi.org/10.1051/vetres:2006056
  19. Ravicini S, Pastor J, Hawley J, Brewer M, Castro-Lopez J, Beall M, et al. Prevalence of selected infectious disease agents in stray cats in Catalonia, Spain. JFMS Open Rep. 2016;2(1):2055116916634109.
  20. Afonso MM, Pinchbeck GL, Smith SL, Daly JM, Gaskell RM, Dawson S, et al. A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity. Vaccine. 2017;35(20):2753-2760. https://doi.org/10.1016/j.vaccine.2017.03.030
  21. Meli ML, Berger A, Willi B, Spiri AM, Riond B, Hofmann-Lehmann R. Molecular detection of feline calicivirus in clinical samples: a study comparing its detection by RT-qPCR directly from swabs and after virus isolation. J Virol Methods. 2018;251:54-60. https://doi.org/10.1016/j.jviromet.2017.10.001
  22. Hou J, Sanchez-Vizcaino F, McGahie D, Lesbros C, Almeras T, Howarth D, et al. European molecular epidemiology and strain diversity of feline calicivirus. Vet Rec. 2016;178(5):114-115. https://doi.org/10.1136/vr.103446
  23. Abd-Eldaim MM, Wilkes RP, Thomas KV, Kennedy MA. Development and validation of a TaqMan real-time reverse transcription-PCR for rapid detection of feline calicivirus. Arch Virol. 2009;154(4):555-560. https://doi.org/10.1007/s00705-009-0337-5
  24. Burgesser KM, Hotaling S, Schiebel A, Ashbaugh SE, Roberts SM, Collins JK. Comparison of PCR, virus isolation, and indirect fluorescent antibody staining in the detection of naturally occurring feline herpesvirus infections. J Vet Diagn Invest. 1999;11(2):122-126. https://doi.org/10.1177/104063879901100203
  25. Mochizuki M, Kawakami K, Hashimoto M, Ishida T. Recent epidemiological status of feline upper respiratory infections in Japan. J Vet Med Sci. 2000;62(7):801-803. https://doi.org/10.1292/jvms.62.801
  26. Henzel A, Brum MC, Lautert C, Martins M, Lovato LT, Weiblen R. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Braz J Microbiol. 2012;43(2):560-568. https://doi.org/10.1590/S1517-83822012000200017
  27. Holst BS, Berndtsson LT, Englund L. Isolation of feline herpesvirus-1 and feline calicivirus from healthy cats in Swedish breeding catteries. J Feline Med Surg. 2005;7(6):325-331. https://doi.org/10.1016/j.jfms.2005.03.002