Journal of Veterinary Science

  • 제21권3호
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  • Pages.50.1-50.16
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway

  • Jin, Xiaohui (The College of Animal Science and Veterinary Medicine, Henan Agricultural University) ;
  • Yuan, Yixin (The College of Animal Science and Veterinary Medicine, Henan Agricultural University) ;
  • Zhang, Chi (The College of Animal Science and Veterinary Medicine, Henan Agricultural University) ;
  • Zhou, Yong (Key Laboratory for Animal-derived Food Safety of Henan Province) ;
  • Song, Yue (Key Laboratory for Animal-derived Food Safety of Henan Province) ;
  • Wei, Zhanyong (The College of Animal Science and Veterinary Medicine, Henan Agricultural University) ;
  • Zhang, Gaiping (The College of Animal Science and Veterinary Medicine, Henan Agricultural University)
  • 투고 : 2020.01.13
  • 심사 : 2020.03.27
  • 발행 : 2020.05.31
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초록

Background: Porcine parvovirus (PPV) is a single-stranded DNA virus that causes porcine reproductive failure. It is of critical importance to study PPV pathogenesis for the prevention and control of the disease. NS1, a PPV non-structural protein, is participated in viral DNA replication, transcriptional regulation, and cytotoxicity. Our previous research showed that PPV can activate nuclear factor kappa B (NF-κB) signaling pathway and then up-regulate the expression of interleukin (IL)-6. Objectives: Herein, the purpose of this study is to determine whether the non-structural protein NS1 of PPV also has the same function. Methods: Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay, western blot, immunofluorescence assay and small interfering RNA (siRNA) were used. Results: Our findings demonstrated that PPV NS1 protein can up-regulate the expression levels of IL-6 and tumor necrosis factor-alpha in a dose-dependent manner. Moreover, PPV NS1 protein was found to induce the phosphorylation of IκBα, then leading to the phosphorylation and nuclear translocation of NF-κB. In addition, the NS1 protein activated the upstream pathways of NF-κB. Meanwhile, TLR2-siRNA assay showed TLR2 plays an important role in the activation of NF-κB signaling pathway induced by PPV-NS1. Conclusions: These findings indicated that PPV NS1 protein induced the up-regulated of IL-6 expression through activating the TLR2 and NF-κB signaling pathways. In conclusion, these findings provide a new avenue to study the innate immune mechanism of PPV infection.

키워드

과제정보

We thank Professor Hui Hu from Henan Agricultural University for revising the manuscript.

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