Journal of Veterinary Science

  • 제21권6호
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  • Pages.85.1-85.6
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Generation of a cold-adapted PRRSV with a nucleotide substitution in the ORF5 and numerous mutations in the hypervariable region of NSP2

  • Do, Van Tan (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Dao, Hoai Thu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2020.03.27
  • 심사 : 2020.09.21
  • 발행 : 2020.11.30
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초록

A cold-adapted porcine reproductive and respiratory syndrome virus (CA-VR2332) was generated from the modified live virus strain VR2332. CA-VR2332 showed impaired growth when cultured at 37℃ with numerous mutations (S731F, E819D, G975E, and D1014N) in the hypervariable region of the NSP2, in which the mutation S731F might play a vital role in viral replication at 30℃. Conserved amino acid sequences of the GP5 protein suggests that CA-VR2332 is a promising candidate for producing an effective vaccine against PRRSV infection. Further studies on replication and immunogenicity in vivo are required to evaluate the properties of CA-VR2332.

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참고문헌

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