Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus

  • Wei, Yanqiu (CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences) ;
  • Yang, Baozhi (College of Animal Sciences and Veterinary Medicine, Guangxi University) ;
  • Li, Yunlong (CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences) ;
  • Duan, Yongcheng (College of Animal Sciences and Veterinary Medicine, Guangxi University) ;
  • Tian, Deyu (CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences) ;
  • He, Baoxiang (College of Animal Sciences and Veterinary Medicine, Guangxi University) ;
  • Chen, Chuangfu (College of Animal Science and Technology, Shihezi University) ;
  • Liu, Wenjun (CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences) ;
  • Yang, Limin (CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences)
  • Received : 2019.11.14
  • Accepted : 2020.04.09
  • Published : 2020.07.31
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Abstract

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

Keywords

Acknowledgement

This study was supported by the Laboratory by the National Key Technologies Research and Development Program of China (No. 2018YFC1200500, 2018YFC1200600) and the National Key Program for Infectious Diseases of China (No. 2016ZX10004222-006). W.J.L. is the principal investigator of the Innovative Research Group of the National Natural Science Foundation of China (Grant No. 81621091).

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