Mycobiology

  • 제48권1호
  • /
  • Pages.70-74
  • /
  • 2020
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Identification and Characterization of Gonatobotryum apiculatum Causing Leaf Spot and Blight on Sinowilsonia henryi

  • Gao, Ying (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Liu, Hai Feng (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Song, Zheng Xing (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Du, Xiao Ying (Department of Plant Protection, College of Agriculture, Yangtze University) ;
  • Deng, Jian Xin (Department of Plant Protection, College of Agriculture, Yangtze University)
  • 투고 : 2019.05.19
  • 심사 : 2019.10.14
  • 발행 : 2020.02.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China. In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei, China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatum based on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analyses indicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the species produced two distinct types of conidia from authentic media, both conidia were described here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spots on S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused by G. apiculatum in China.

키워드

참고문헌

  1. Ying JS. An analysis of the flora of Qinling mountain range: its nature, characteristics, and origins. Acta Phytotaxon Sin. 1994;32:389-410.
  2. Fu LK. China plant red data book-rare and endangered plants. Beijing: Science Press; 1992.
  3. Fu ZJ. The study of the geographica distribufion and the ecological property on Sinowilsonia henryi. J Baoji Teach Coll. 1993;1:86-89.
  4. Xiong XY. Variation of stamen number and form of Sinowilsonia and its systematic significance. Anhui Agric Sci Bull. 2008;14:92-93.
  5. Huang B, Sun QM, Liu XJ. Introduction and cultivation of rare tree species Sinowilsonia henryi in Nanjing. Nor Hortic. 2011;20:90-91.
  6. Endress PK. A supragenetic taxonomic classification of the Hamamelidaceae. Taxon. 1989;38(3):371-376. https://doi.org/10.2307/1222267
  7. Zhang Q, Shi SH, Huang YL, et al. ITS sequences of Hamamelidoideae and their systematic significance. J Wuhan Bot Res. 2001;19:445-448. https://doi.org/10.3969/j.issn.2095-0837.2001.06.001
  8. Linghu YW, Zhang Y, Li B, et al. Effects of three plant hormones on seed germination of Sinowilsonia henryi under sterile condition. Shaanxi For Sci Technol. 2009;3:22-25.
  9. Zhou TH, Wu KX, Qian ZQ, et al. Genetic diversity of the threatened Chinese endemic plant, Sinowilsonia henryi Hemsi. (Hamamelidaceae), revealed by inter-simple sequence repeat (ISSR) markers. Biochem Syst Ecol. 2014;56:171-177. https://doi.org/10.1016/j.bse.2014.05.017
  10. Cenis J. Rapid extraction of fungal DNA for PCR amplification. Nucl Acids Res. 1992;20(9):2380. https://doi.org/10.1093/nar/20.9.2380
  11. White TJ, Bruns TD, Lee SB, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-322.
  12. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172(8):4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  13. Schoch CL, Crous PW, Groenewald JZ, et al. A class-wide phylogenetic assessment of Dothideomycetes. Study Mycol. 2009;64:1-15. https://doi.org/10.3114/sim.2009.64.01
  14. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  15. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006;22(21):2688-2690. https://doi.org/10.1093/bioinformatics/btl446
  16. Kendrick WB, Cole GT, Bhatt GC. Conidium ontogeny in hyphomycetes. Gonatobotryum apiculatum and its botryose blastospores. Can J Bot. 1968;46(5):591-596. https://doi.org/10.1139/b68-085
  17. Jacob M, Bhat DJ. Two new endophytic conidial fungi from India. Cryptogam Mycol. 2000;21(2):81-88. https://doi.org/10.1016/S0181-1584(00)00116-0
  18. Walker JC, Minter AW. Taxonomy of Nematogonum, Gonatobotrys, Gonatobotryum, and Gonatorrhodiella. Trans Br Mycol Soc. 1981;77(2):299-319. https://doi.org/10.1016/s0007-1536(81)80032-0
  19. Dorfelt H, Schmidt AR. A conifer seeding with two herbicolous fungi from the Baltic amber forest. Bot J Lin Soc. 2007;155(4):449-456. https://doi.org/10.1111/j.1095-8339.2007.00728.x
  20. Shou HR, Huang YX, Zang W, et al. A new recorded of Gonatobotryum in China. Microbiol China. 2015;40:1984-1987.
  21. Barnett HL, Hunter BB. Illustrated genera of imperfect fungi. 4th ed. St. Paul Minnesota: The American Phytopathological Society Press; 1998; p.1-218.