Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of puerarin on the Akt signaling pathway in bovine preadipocyte differentiation

  • Yun, Jinyan (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences) ;
  • Yu, Yongsheng (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences) ;
  • Zhou, Guoli (College of Life Science, Liaocheng University) ;
  • Luo, Xiaotong (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences) ;
  • Jin, Haiguo (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences) ;
  • Zhao, Yumin (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences) ;
  • Cao, Yang (Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences)
  • Received : 2019.01.02
  • Accepted : 2019.05.04
  • Published : 2020.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro. Methods: Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with oil red O. The content of triacylglycerol was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α, were measured using quantitative real-time polymerase chain reaction and western blotting, respectively. Results: The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPARγ (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPARγ expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway. Conclusion: Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.

Keywords

References

  1. Alessi MC, Peiretti F, Morange P, Henry M, Nalbone G, Juhan-Vague I. Production of plasminogen activator inhibitor 1 by human adipose tissue: Possible link between visceral fat accumulation and vascular disease. Diabetes 1997;46:860-7. https://doi.org/10.2337/diab.46.5.860
  2. Avram MM, Avram AS, James WD. Subcutaneous fat in normal and diseased states 3. Adipogenesis: from stem cell to fat cell. J Am Acad Dermatol 2007;56:472-92. https://doi.org/10.1016/j.jaad.2006.06.022
  3. Camp HS, Ren D, Leff T. Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol Med 2002;8:442-7. https://doi.org/10.1016/S1471-4914(02)02396-1
  4. Feve, B. Adipogenesis: Cellular and molecular aspects. Best Pract Res Clin Endocrinol Metab 2005;19:483-99. https://doi.org/10.1016/j.beem.2005.07.007
  5. Lundgren CH, Brown SL, Nordt TK, Sobel BE, Fujii S. Elaboration of type-1 plasminogen activator inhibitor from adipocytes. A potential pathogenetic link between obesity and cardiovascular disease. Circulation 1996;93:106-10. https://doi.org/10.1161/01.CIR.93.1.106
  6. Macdougald OA, Mandrup S. Adipogenesis: forces that tip the scales. Trends Endocrinol Metab 2002;13:5-11. https://doi.org/10.1016/S1043-2760(01)00517-3
  7. Rosen ED, Spiegelman BM. Adipocytes as regulators of energy balance and glucose homeostasis. Nature 2006;444:847-53. https://doi.org/10.1038/nature05483
  8. Margawati ET. A global strategy of using molecular genetic information to improve genetics in livestock. Reprod Domest Anim 2012;47:7-9. https://doi.org/10.1111/j.1439-0531.2011.01957.x
  9. Robelin J. Cellularity of bovine adipose tissues: developmental changes from 15 to 65 percent mature weight. J Lipid Res 1981; 22:452-7. https://doi.org/10.1016/S0022-2275(20)34959-2
  10. Cianzio DS, Topel DG, Whitehurst GB, Beitz DC, Self HL. Adipose tissue growth and cellularity: changes in bovine adipocyte size and number. J Anim Sci 1985;60:970-6. https://doi.org/10.2527/jas1985.604970x
  11. Du M, Yin J, Zhu MJ. Cellular signaling pathways regulating the initial stage of adipogenesis and marbling of skeletal muscle. Meat Sci 2010;86:103-9. https://doi.org/10.1016/j.meatsci. 2010.04.027
  12. Jung HW, Kang AN, Kang SY, Park YK, Song MY. The root extract of Pueraria lobata and its main compound, puerarin, prevent obesity by increasing the energy metabolism in skeletal muscle. Nutrients 2017;9:E33. https://doi.org/10.3390/nu9010033
  13. Zheng G, Lin L, Zhong S, Zhang Q, Li D. Effects of puerarin on lipid accumulation and metabolism in high-fat diet-fed mice. PloS One 2015;10:e0122925. https://doi.org/10.1371/journal.pone.0122925
  14. Wang N, Wang X, Cheng W, Cao H, Zhang P, Qin L. Puerarin promotes osteogenesis and inhibits adipogenesis in vitro. Chin Med 2013;8:17. https://doi.org/10.1186/1749-8546-8-17
  15. Lee OH, Seo DH, Park CS, Kim YC. Puerarin enhances adipocyte differentiation, adiponectin expression, and antioxidant response in 3T3-L1 cells. BioFactors 2010;36:459-67. https://doi.org/10.1002/biof.119
  16. Xu ME, Xiao SZ, Sun YH, Zheng XX, Ou-Yang Y, Guan C. The study of anti-metabolic syndrome effect of puerarin in vitro. Life Sci 2005;77:3183-96. https://doi.org/10.1016/j.lfs. 2005.03.036
  17. Prasain JK, Peng N, Rajbhandari R, Wyss JM. The Chinese Pueraria root extract (Pueraria lobata) ameliorates impaired glucose and lipid metabolism in obese mice. Phytomedicine 2012;20:17-23. https://doi.org/10.1016/j.phymed.2012.09.017
  18. Linhart HG, Ishimura-Oka K, DeMayo F, et al. C/EBPalpha is required for differentiation of white, but not brown, adipose tissue. Proc Natl Acad Sci USA 2001;98:12532-7. https://doi.org/10.1073/pnas.211416898
  19. Lefterova MI, Zhang Y, Steger DJ, et al. PPAR gamma and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale. Genes Dev 2008;22:2941-52. https://doi.org/10.1101/gad.1709008
  20. Sahiel AR, Pessin JE. Signaling pathways in insulin action: molecular targets of insulin resistance. J Clin Invest 2000;106:165-9. https://doi.org/10.1172/JCI10582
  21. Franke TF, Kaplan DR, Cantley LC. PI3K: downstream AKTion blocks apoptosis. Cell 1997;88:435-7. https://doi.org/10.1016/S0092-8674(00)81883-8
  22. Burgering BM, Coffer PJ. Protein kinase B(c-Akt) in phosphatidylinositol- 3-OH kinase signal transduction. Nature 1995;376:599-602. https://doi.org/10.1038/376599a0
  23. Hajduch E, Litherland GJ, Hundal HS. Protein kinase B (PKB/Akt) -- a key regulator of glucose transport? FEBS Lett 2001;492:199-203. https://doi.org/10.1016/S0014-5793(01)02242-6
  24. Cross DA, Alessi DR, Cohen P, Andjelkovich M, Hemmings BA. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 1995;378:785-9. https://doi.org/10.1038/378785a0
  25. Peng XD, Xu PZ, Chen ML, et al. Dwarfism, impaired skin development, skeletal muscle atrophy, delayed bone development, and impeded adipogenesis in mice lacking Akt1 and Akt2. Genes Dev 2003;17:1352-65. https://doi.org/10.1101/gad.1089403
  26. Kim SP, Ha JM, Yun SJ, et al. Transcriptional activation of peroxisome proliferator- activated receptor-gamma requires activation of both protein kinase A and Akt during adipocyte differentiation. Biochem Biophys Res Commun 2010;399:55-9. https://doi.org/10.1016/j.bbrc.2010.07.038
  27. Smith PJ, Wise LS, Berkowitz R, Wan C, Rubin CS. Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes. J Biol Chem 1988;263:9402-8. https://doi.org/10.1016/S0021-9258(19)76555-7
  28. Cornelius P, MacDougald OA, Lane MD. Regulation of adipocyte development. Annu Rev Nutr 1994;14:99-129. https://doi.org/10.1146/annurev.nu.14.070194.000531
  29. Kohn AD, Summers SA, Birnbaum MJ, Roth RA. Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation. J Biol Chem 1996;271:31372-8. https://doi.org/ 10.1074/jbc.271.49.31372
  30. Vlahos CJ, Matter WF, Hui KY, Brown RF. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J Biol Chem 1994;269:5241-8. https://doi.org/10.1016/S0021-9258(17)37680-9

Cited by

  1. Puerarin improved growth performance and postmortem meat quality by regulating lipid metabolism of cattle under hot environment vol.92, pp.1, 2020, https://doi.org/10.1111/asj.13543