Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene

  • Zhang, Xiaodong (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Tao, Qiangqiang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Shang, Jinnan (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Xu, Yiliang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhang, Liang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Ma, Yingchun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Zhu, Weihua (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yang, Min (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Ding, Yueyun (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Yin, Zongjun (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2019.03.03
  • Accepted : 2019.05.31
  • Published : 2020.04.01
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Abstract

Objective: Apoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs. Methods: The pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzyme-linked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor. Results: Results showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs. Conclusion: This study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.

Keywords

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