Korean Journal of Audiology (대한청각학회지)

The Korean Audiology Society (대한청각학회)
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Comparison of Autophagy mRNA Expression between Chronic Otitis Media With and Without Cholesteatoma

  • Jung, Junyang (Departments of Anatomy and Neurobiology, School of Medicine, KyungHee University) ;
  • Jung, Su Young (Department of Otorhinolaryngology-Head and Neck Surgery, Myongji Hospital, Hanyang University College of Medicine) ;
  • Kim, Myung Gu (Department of Otorhinolaryngology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Kim, Young Il (Medical Science Research Institute, KyungHee University Medical Center) ;
  • Kim, Sang Hoon (Department of Otorhinolaryngology, School of Medicine, KyungHee University) ;
  • Yeo, Seung Geun (Medical Science Research Institute, KyungHee University Medical Center)
  • Received : 2020.03.06
  • Accepted : 2020.04.30
  • Published : 2020.10.20
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Abstract

Background and Objectives: Autophagy is known to be associated with pathogen infection. However, the expression of autophagy-related proteins has not been studied in chronic otitis media without cholesteatoma (COM) or with cholesteatoma (CholeOM). This study aimed to determine whether there is a difference between COM and CholeOM in autophagy-related gene mRNA expression. Subjects and Methods: For 47 patients with chronic otitis media, the inflammatory tissues were classified into granulation tissue (COM) or cholesteatoma (CholeOM) according to biopsy results. Results: PI3K mRNA expression (COM vs. CholeOM, mean±SD, 0.009±0.010 vs. 0.003±0.004; p=0.004) was lower, whereas Beclin-1 mRNA expression (0.089±0.107 vs. 0.176±0.163; p=0.034) was higher in the CholeOM group. Expression of PI3K mRNA in the CholeOM group was lower than that in the COM subgroups with presence of bacteria (0.022±0.019 vs. 0.001±0.001; p=0.001), otorrhea (0.049±0.068 vs. 0.003±0.004; p=0.004), and hearing loss over 40 dB (0.083±0.130 vs. 0.003±0.004; p=0.005). Conclusions: The data suggested that different autophagy proteins play important roles in chronic otitis media according to the presence or absence of cholesteatoma.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2018R1A6A1A03025124).

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