Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Purification and Characterization of an Antibacterial Substance from Aerococcus urinaeequi Strain HS36

  • Sung, Ho Sun (Department of Applied Microbiology and Biotechnology, Yeungnam University) ;
  • Jo, Youl-Lae (Department of Applied Microbiology and Biotechnology, Yeungnam University)
  • Received : 2019.10.10
  • Accepted : 2019.10.30
  • Published : 2020.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis, was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus urinaeequi based on the characteristics of the genus according to Bergey's Manual of Systematic Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain HS36 in shaking culture were higher than those in static culture, while the optimal pH and temperature for antibacterial activity were 7.0 and 30℃, respectively. The active antibacterial substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain HS36 was stable after incubation for 1 h at 100℃. Although its antibacterial activity was optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.

Keywords

References

  1. Cordero H, Esteban MA, Cuesta A. 2014. Use of probiotic bacteria against bacterial and viral infections in shellfish and fish aquaculture. Intech. Open Sci. 8: 239-265.
  2. Abarike ED, Jian J, Tang J, Cai J, Yu H, Lihua C, et al. 2018. Influence of traditional Chinese medicine and Bacillus species (TCMBS) on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus. Aquac. Res. 49: 2366-2375. https://doi.org/10.1111/are.13691
  3. Baker-Austin C, Oliver JD, Alam M, Ali A, Waldor MK, Qadri F, et al. 2018. Vibrio spp. infections. Nat. Rev. Dis. Primers. 4: 8.
  4. Chatterjee S, Haldar S. 2012. Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. J. Marine Sci. Res. Dev. S: 1.
  5. Amalina NZ, Dzarifah Z, Amal MNA, Yusof MT, Zamri- Saad M, Al-saari N, et al. 2019. Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia. Aquaculture Research.
  6. Noga EJ. 2010. Fish Disease: Diagnosis and Treatment, pp. 65-68. 2th Ed. Wiley-Blackwell.
  7. Miranda CD, Tello A, Keen PL. 2013. Mechanisms of antimicrobial resistance in finfish aquaculture environments. Front Microbiol. 4: 233. https://doi.org/10.3389/fmicb.2013.00233
  8. Sapkota A, Sapkota AR, Kucharski M, Burke J, McKenzie S, Walker P, et al. 2008. Aquaculture practices and potential human health risks: current knowledge and future priorities. Environ. Int. 34: 1215-1226. https://doi.org/10.1016/j.envint.2008.04.009
  9. Nwachi OF 2013. An overview of the importance of probiotics in aquaculture. J. Fish Aquat. Sci. 8: 30-32. https://doi.org/10.3923/jfas.2013.30.32
  10. Tan L TH., Chan KG, Lee LH, Goh BH. 2016. Streptomyces bacteria as potential probiotics in aquaculture. Front. Microbiol. 7: 79. https://doi.org/10.3389/fmicb.2016.00079
  11. Tuan TN, Duc PM, Hatai K. 2013. Overview of the use of probiotics in aquaculture. Inter. J. Res. Fish. Aquac. 3: 89-97.
  12. Cha JH, Yang SY, Woo SH, Song JW, Oh DH, Lee KJ. 2012. Effects of dietary supplementation with Bacillus sp. on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in olive flounder Paralichthys olivaceus. Kor. J. Fish Aquat. Sci. 45: 35-42. https://doi.org/10.5657/KFAS.2012.0035
  13. Todorov SD, de Melo Franco BDG, Tagg JR. 2019. Bacteriocins of Gram-positive bacteria having activity spectra extending beyond closely-related species. Benef. Microbes. 10: 315-328. https://doi.org/10.3920/BM2018.0126
  14. Yang SC, Lin CH, Sung CT, Fang JY. 2014. Antibacterial activities of bacteriocins: application in foods and pharmaceuticals. Front. Microbiol. 5: 241.
  15. Hussein AR, Khalaf ZZ, Samir Z, Samir R. 2018. Antibacterial activity of crud Bacteriocin-like substance against food borne bacterial pathogens. Iraqi. J. Sci. 59: 16-24.
  16. De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, et al. 2009. Bergey's Manual of Systematic Bacteriology: Volume 3, pp. 533-536. 2th Ed. The firmicutes.
  17. Crowley S, Mahony J, van Sinderen D. 2013. Current perspectives on antifungal lactic acid bacteria as natural biopreservatives. Trends Food Sci. Technol. 33: 93-109. https://doi.org/10.1016/j.tifs.2013.07.004
  18. Ghanbari MC, Jami M, Domig KJ, Kneifel W. 2013. Seafood biopreservation by lactic acid bacteria - A review. LWT-Food Sci. Technol. 54: 315-324. https://doi.org/10.1016/j.lwt.2013.05.039
  19. Perez RH, Zendo T, Sonomoto K. 2014. Novel bacteriocins from lactic acid bacteria (LAB): various structures and applications. Microb. Cell Fact. 13: S3. https://doi.org/10.1186/1475-2859-13-S1-S3
  20. Rattanachaikunsopon P, Phumkhachorn P. 2006. Isolation and preliminary characterization of a bacteriocin produced by Lactobacillus plantarum N014 isolated from nham, a traditional Thai fermented pork. J. Food Prot. 69: 1937-1943. https://doi.org/10.4315/0362-028X-69.8.1937
  21. Klaenhammer TR. 1993. Genetics of bacteriocin produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39-85. https://doi.org/10.1111/j.1574-6976.1993.tb00012.x
  22. Ahn JE, Kim JK, Lee HR, Eom HJ, Han NS. 2012. Isolation and characterization of a bacteriocin-producing Lactobacillus sakei B16 from Kimchi. J. Kor. Soc. Food Sci. Nutr. 41: 721-726. https://doi.org/10.3746/JKFN.2012.41.5.721
  23. Singh PK, Chittpurna A, Sharma V, Paril, PB, Suresh K. 2012. Identification, purification and characterization of Laterosporulin, a Novel Bacteriocin Produced by Brevibacillus sp. strain Gl-9. PLoS One 7: e31498. https://doi.org/10.1371/journal.pone.0031498
  24. Chen SW, Liu CH, Hu SY. 2018. Dietary administration of probiotic Paenibacillus ehimensis NPUST1 with bacteriocinlike activity improves growth performance and immunity against Aeromonas hydrophila and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 84: 695-703. https://doi.org/10.1016/j.fsi.2018.10.059
  25. Jimenez-Diaz R, Rios-Sanchez RM, Desmazeaud M, Ruiz-Barba J, Piard JC. 1993. Plantaricin S and T new bacteriocins produced by Lactobacillus plantarum LPCOlO isolated from a green olive fermentation. Appl. Environ. Microbiol. 59: 1416-1424. https://doi.org/10.1128/aem.59.5.1416-1424.1993
  26. Huh CK, Hwang TY. 2016. Identification of antifungal substances of Lactobacillus sakei subsp. ALI033 and antifungal activity against Penicillium brevicompactum strain FI02. Prev. Nutr. Food Sci. 21: 52-56. https://doi.org/10.3746/pnf.2016.21.1.52
  27. Ahn H, Kim J, Kim WJ. 2017. Isolation and characterization of bacteriocin-producing Pediococcus acidilactici HW01 from malt and its potential to control beer spoilage lactic acid bacteria. Food Control. 80: 59-66. https://doi.org/10.1016/j.foodcont.2017.04.022
  28. Ryan MP, Rea MC, Hill C, Ross RP. 1996. An application in Cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad spectrum bacteriocin, lacticin 3147. Appl. Environ. Microbiol. 62: 612-619. https://doi.org/10.1128/aem.62.2.612-619.1996
  29. Hechard Y, Derijard B, Letellier F, Cenatiempo Y. 1992. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J. Gen. Microbiol. 138: 2725-2731. https://doi.org/10.1099/00221287-138-12-2725
  30. Belfiore C, Castellano P, Vignolo G. 2007. Reduction of Escherichia coli population following treatment with bacteriocins from lactic acid bacteria and chelators. Food Microbiol. 24: 223-229. https://doi.org/10.1016/j.fm.2006.05.006

Cited by

  1. Probiotics Modulate Intestinal Motility and Inflammation in Zebrafish Models vol.17, pp.6, 2020, https://doi.org/10.1089/zeb.2020.1877
  2. Decoding the intestinal microbiota repertoire of sow and weaned pigs using culturomic and metagenomic approaches vol.63, pp.6, 2021, https://doi.org/10.5187/jast.2021.e124