Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Trehalose Protects the Probiotic Yeast Saccharomyces boulardii against Oxidative Stress-Induced Cell Death

  • Moon, Ji Eun (Department of Food and Biotechnology, Korea University) ;
  • Heo, Wan (Institutes of Natural Sciences, Korea University) ;
  • Lee, Sang Hoon (Department of Food and Biotechnology, Korea University) ;
  • Lee, Suk Hee (Department of Molecular Medicine, Cell and Matrix Research Institute, Kyungpook National University School of Medicine) ;
  • Lee, Hong Gu (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, Jin Hyup (Department of Food and Biotechnology, Korea University) ;
  • Kim, Young Jun (Department of Food and Biotechnology, Korea University)
  • Received : 2019.06.18
  • Accepted : 2019.09.18
  • Published : 2020.01.28
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Abstract

Saccharomyces boulardii is the only probiotic yeast with US Food and Drug Administration approval. It is routinely used to prevent or treat acute diarrhea and other gastrointestinal disorders, including the antibiotic-associated diarrhea caused by Clostridium difficile infections. The formation of reactive oxygen species (ROS), specifically H2O2 during normal aerobic metabolism, contributes to programmed cell death and represents a risk to the viability of the probiotic microbe. Moreover, a loss of viability reduces the efficacy of the probiotic treatment. Therefore, inhibiting the accumulation of ROS in the oxidant environment could improve the viability of the probiotic yeast and lead to more efficacious treatment. Here, we provide evidence that supplementation with a non-reducing disaccharide, namely trehalose, enhanced the viability of S. boulardii exposed to an oxidative environment by preventing metacaspase YCA1-mediated programmed cell death through inhibition of intracellular ROS production. Our results suggest that supplementation with S. boulardii together with trehalose could increase the viability of the organism, and thus improve its effectiveness as a probiotic and as a treatment for acute diarrhea and other gastrointestinal disorders.

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References

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