한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제35권1호
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  • Pages.102-111
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  • 2020
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Nitric Oxide-induced Protein S-nitrosylation Causes Mitochondrial Dysfunction and Accelerates Post-ovulatory Aging of Oocytes in Cattle

  • Niu, Ying-Jie (Department of Animal Science, Chungbuk National University) ;
  • Zhou, Dongjie (Department of Animal Science, Chungbuk National University) ;
  • Zhou, Wenjun (Department of Animal Science, Chungbuk National University) ;
  • Nie, Zheng-Wen (Department of Animal Science, Chungbuk National University) ;
  • Kim, Ju-Yeon (Department of Animal Science, Chungbuk National University) ;
  • Oh, YoungJin (Chung Cheong Buk-Do Institute of Livestock and Veterinary Research) ;
  • Lee, So-Rim (Chung Cheong Buk-Do Institute of Livestock and Veterinary Research) ;
  • Cui, Xiang-Shun (Department of Animal Science, Chungbuk National University)
  • 투고 : 2020.03.17
  • 심사 : 2020.03.24
  • 발행 : 2020.03.31
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초록

Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

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참고문헌

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