Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Anti-inflammatory and Anti-itching Effects of Herbal Medicine Complex Extracts(NI-01)

한약복합추출물(NI-01)의 항염증 및 소양감 억제 효과

  • Kim, Hyun-A (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Kim, Suk-Kyung (Yushang) ;
  • Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 김현아 (계명대학교 자연과학대학 공중보건학과) ;
  • 김숙경 (유상그린유에스(주)) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학과)
  • Received : 2020.07.27
  • Accepted : 2020.11.24
  • Published : 2020.12.25
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Abstract

The purpose of this study was to investigate the possibility of herbal medicine complex extract (NI-01), which were prepared from 6 natural materials (Cinnamomum cassia Blume, Lonicerae Flos, Paeonia suffruticosa Andrews, Arctium lappa Linne, Schzandra chinesis Bailon, Elsholtzia ciliata Hylander), as a functional material for inhibition of atopic dermatitis. anti-oxidative activity was confirmed by measuring DPPH electron donating ability and ABTS+ radical scavenging ability. Cytotoxicity and NO inhibition were measured using RAW 264.7 cells to confirm anti-inflammatory efficacy. The test substance was orally administered to the pruritus-induced ICR mice to confirm the inhibition of pruritus. The bovine cornea opacity and permeability (BCOP) assay was performed to confirm safety for irritation. NI-01 showed high antioxidant activity in DPPH and ABTS+ methods. In the anti-inflammatory effect tests with RAW 264.7 cells, NO production was inhibited at NI-01 concentrations of 50 (14.9%) and 100 (4.2%) ㎍/mL, which indicated that the anti-inflammatory effect was increased in a concentration-dependent manner. NI-01 also showed anti-itching effect after inducing of itching by compound 48/80 in ICR mice. NI-01 was proved to be a non-irritant substance in BCOP assay. The results of this study suggested that the herbal medicine combined extract (NI-01) has high antioxidant, anti-inflammatory and anti-itching effects, and safety for irritation. Therefore, herbal medicine complex extract (NI-01) is thought to be highly applicable for the inhibitory ingredients of the atopic dermatitis.

Keywords

References

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