Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Palmijihwang-tang Alleviates Cisplatin-induced Nephrotoxicity through Inhibiting ROS Production and p53 Activation

팔미지황탕(八味地黃湯)의 ROS 생성 및 p53 활성 조절을 통한 시스플라틴 신장독성 완화효과

  • Ju, Sung-Min (Department of Pathology, College of Korean Medicine, Wonkwnag University) ;
  • Park, Seo-Hee (Department of Preventive Medicine, College of Korean Medicine, Wonkwnag University) ;
  • Chong, Myong-Soo (Department of Preventive Medicine, College of Korean Medicine, Wonkwnag University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Korean Medicine, Wonkwnag University)
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 박서희 (원광대학교 한의과대학 예방의학교실) ;
  • 정명수 (원광대학교 한의과대학 예방의학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Received : 2020.07.10
  • Accepted : 2020.08.13
  • Published : 2020.08.25
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Abstract

Palmijihwang-tang is an herbal formula frequently used to treat many symptoms, such as lumbago, pollakiuria, cold hands and feet, nephritis, sterilitas virilis, and prostatic disorders. The aim of this study was to investigate the effects of Palmijihwang-tang on cisplatin-induced nephrotoxicity in rat kidney proximal tubular NRK-52E cells. NRK-52E cells were treated with Palmijihwang-tang in absence or presence of 30 µM cisplatin for 12 or 24 h. Palmijihwang-tang at concentrations of 50-800 ㎍/ml did not change the cell viability in NRK-52E cells, and showed no significant toxicity. Palmijihwang-tang at concentrations of 400 and 800 ㎍/ml significantly increased the cell viability and reduced apoptotic cells in NRK-52E cells exposed to cisplatin. Also, Palmijihwang-tang markedly inhibited cisplatin-induced caspase-3 activation, PARP cleavage, ROS production and p53 activation in NRK-52E cells. Furthermore, Palmijihwang-tang did not interfere with the antitumor activity of cisplatin in AGS and A549 cancer cells. Particularly, Palmijihwang-tang enhanced antitumor activity of cisplatin in A549 cells. Taken together, these results suggest that Palmijihwang-tang ameliorated cisplatin-induced nephrotoxicity through reduction of ROS production and p53 activation, and did not interrupt antitumor efficacy of cisplatin against cancer cells.

Keywords

References

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