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Molecular survey of Dirofilaria immitis in mosquitoes collected from parks the in the Incheon metropolitan city in Korea

인천지역 공원 채집 모기에서 심장사상충 감염도 조사

  • Jeong, Cheol (Incheon Metropolitan City Institute of Health & Environment) ;
  • Song, Jae-Sung (Incheon Metropolitan City Institute of Health & Environment) ;
  • Kim-Jeon, Myung-Deok (Incheon Metropolitan City Institute of Health & Environment) ;
  • Kim, Kyoung-Ho (Incheon Metropolitan City Institute of Health & Environment) ;
  • Lee, Jung-Goo (Incheon Metropolitan City Institute of Health & Environment) ;
  • Lee, Sung-Mo (Incheon Metropolitan City Institute of Health & Environment)
  • 정철 (인천광역시 보건환경연구원) ;
  • 송재성 (인천광역시 보건환경연구원) ;
  • 김명덕 (인천광역시 보건환경연구원) ;
  • 김경호 (인천광역시 보건환경연구원) ;
  • 이정구 (인천광역시 보건환경연구원) ;
  • 이성모 (인천광역시 보건환경연구원)
  • Received : 2019.06.12
  • Accepted : 2020.03.13
  • Published : 2020.03.30

Abstract

Unlike previous studies that reported the infection rates of Dirofilaria immitis in companion dogs, D. immitis genes were tested directly on mosquitoes to see how many mosquitoes actually carry larvae. Mosquito samples were collected from six parks in Incheon from March to August 2018. A total of 18,589 mosquitoes was collected, classified by type and then made 575 sample pools based on 50 mosquitoes. The PCR method was used to detect Dirofilaria immitis genes of sample pools. Dirofilaria immitis genes were detected in 7 of the 575 pools, and these positive mosquito samples were collected in 4 out of the 6 parks. The types of detected mosquitoes were Aedes dorsalis and Ochlerotatus koreanus which have been identified to act as a vector for heart worm propagation in Incheon. Based on these results, it was concluded that D. immitis genes were found in mosquitoes in the neighborhood park.

Keywords

References

  1. 문희주. 1996. 인체기생충학. 고문사: 68-69.
  2. 박응복, 이희성. 1962. 진주지방 축견의 견사상충 조사. 진주농대연구보고 1: 34-58.
  3. 유재승, 신이현, 주영란. 2015. 감염병 매개모기 원격 계측기 개발. 주간 건강과 질병. 8(38): 902-905.
  4. 이병인, 조영웅. 1996. 개심장사상충증(canine heartworm disease). 대한수의사회지 32(8): 495-502.
  5. 이재구. 1987. 최신수의기생충학. 대한교과서주식회사: 234-239.
  6. 이종훈, 심상원, 김희 등. 2003. 충남지역 집단 번식농장 사육견의 심장사상충 감염률 조사. 한가위지 26(1): 19-26.
  7. 장승익, 송운재, 하숙희 등. 2004. 대전지역 사육견의 심장사상충 감염실태 조사. 한가위지 27(2): 133-137.
  8. Adkins RB Jr, Dao AH. 1984. Pulmonary dirofilariasis: a diagnostic challenge. South Med J 77(3): 327-374. https://doi.org/10.1097/00007611-198403000-00015
  9. Cancrini G. Pietrobulli M, Frangipane di Redalbono AF, Tampieri MP, della Torre A. 1995. Development of Dirofilaria and Setaria nematodes in Ades albopictus. Parasitologia 37: 141-145.
  10. Cifferi F. 1982. Human pulmonary dirofilariasis in the United States: A critical review. Am J Trop Med Hyg 31(2): 302-308. https://doi.org/10.4269/ajtmh.1982.31.302
  11. Jubb KVF, Kennedy PC, Palmer N. 1993. Pathology of domestic animal. 4ed 3: 68-71.
  12. Kim MK, Kim CK, Yeom BW, Park SH, Choi SY, Choi JS. 2002. The first human case of hepatic dirofilariasis. J Korean Med Sci 17: 686-690. https://doi.org/10.3346/jkms.2002.17.5.686
  13. Kim TK, Jo MH, Park MN, Jo EJ, Nam TS, Son SK, Heo JH. 2009. Prevalence of heartworm infection among dogs on breeding farms in southern Gyeongnam area. Korean J Vet Serv 32(4): 369-375.
  14. Koh BRD, Na HM, Jang MS, Kim JY, Park SD. 2007. Investigation of canine dirofilariasis and brucellosis in free roaming dogs from public animal shelters in Gwangju area. Korean J Vet Serv 30(1): 155-164.
  15. Lee JW. Eum SS, Park IG, Bea JJ, Joung DS. 2005. Prevalence of heartworms, scabies and demodicosis of pet dogs in Jeonju area. Korean J Vet Serv 28(1): 39-47.
  16. Lee KJ, Park GM, Yong TS. 2000. The first Korean case of human pulmonary dirofilariasis. Yonsei Med J 41: 285-288. https://doi.org/10.3349/ymj.2000.41.2.285
  17. Lee SE, Kim HC, Chong ST, Klein TA, Lee WJ. 2007. Molecular survey of Dirofilaria immitis and Dirofilaria repens by direct PCR for wild caught mosquitoes in the Republic of Korea. Vet Parasitol 148: 149-155. https://doi.org/10.1016/j.vetpar.2007.04.015
  18. Lee SM, Hwang HS, Kim HJ. 1999. Studies of infection rate and histopathological findings of canine heartworms in Inchon city. Korean J Vet Serv 22(1): 25-35.
  19. Liu J, Song KH, Lee SE, Lee JY, Lee JI, Hayasaki M, You MJ, Kim DH. 2005. Seorological and molecular survey of Dirofilaria immitis infection in stray cats in Gyeonggi Province, South Korea. Vet Parasitol. 130: 125-129. https://doi.org/10.1016/j.vetpar.2005.03.026
  20. Monchy D. Levenes H, Guegan H. et al. 1993. Pulmonary dirofilariasis(French). Med Trop 53(3): 366-371.
  21. Mutaz H, Ozdemir A, Schaefer RC. 2004. Case of the month. A case report of human pulmonary dirofilariasis in Arkansas. J Ark Med Soc 100(7): 240-242.
  22. Pampiglione S, Rivasi F. 2000. Human dirofilariasis due to Dirofilaria (Nochtiella) repens: an update of world literature from 1995 to 2000. Parasitologia 42: 231-254.
  23. Park CE. 2010. Epidemiological survey on prevalence of Dirofilaria immitis infection in dogs of Ulsan area. Korean J Vet Serv 33(2): 157-165.
  24. Prescott CW. 1986. Diseases of dogs. The University of Sydney Post-Graduate Foundation in Veterinary Science 7: 52-54.
  25. Song KH, Lee SE, Hayasaki M, Shiramizu K, Kim DH, Cho KW. 2003. Seroprevalence of canine dirofilariosis in South Korea. Vet Parasitol 114: 231-236. https://doi.org/10.1016/S0304-4017(03)00137-7
  26. Watt KJ, Courteny CH, Reddy GR. 1999. Development of a PCR and probe-based test for the sensitive and specific detection of the dog heartworm Dirofilaria immitis, in its mosquito intermediate host. Mol Cell Probes 13: 425-430. https://doi.org/10.1006/mcpr.1999.0270
  27. Yoshimura H, Yokogawa M. 1970. Dirofilaria causing infarct in human lung. Am J Trop Med Hyg 19(1): 63-67. https://doi.org/10.4269/ajtmh.1970.19.63