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Foraging behaviour and preference of pollen sources by honey bee (Apis mellifera) relative to protein contents

  • Ghosh, Sampat (Agricultural Science and Technology Research Institute, Andong National University) ;
  • Jeon, Hyejin (Gyeongbuk Agri-Food Distribution Innovation Committee) ;
  • Jung, Chuleui (Agricultural Science and Technology Research Institute, Andong National University)
  • Received : 2019.11.21
  • Accepted : 2020.01.07
  • Published : 2020.03.31

Abstract

Background: Pollen is an important source of protein and lipids for many animals including honey bees. In order to understand the foraging behaviour of honey bee colonies and preference among the available floral resources, pollen collections from three experimental healthy colonies of honey bees were analysed in the month of June. Results: The amount of pollen collections were related to the colony's need which was indicated by the number of larval and adult bees present in the hive. Interesting was the sequence of pollen collection from different floral sources. All honey bee colonies collected pollens from Trifolium repens first, then Erigeron annus and the third choice was Coreopsis drummondii and Oenothera biennis flowers. Total protein content of Trifolium pollen was the highest (20.0 g/100 g DM), and the others were in the range of 8.9-11.4 g/100 g DM. Conclusion: The results indicated that the first criteria for honey bee foraging preference of pollens would be the nutritional contents of protein and the resource availability of the lesser nutritious floral sources. This information can help pollinator protection programmes of habitat manipulation using flowering plants for nectar and pollen sources.

Keywords

References

  1. Abou-Shaara HF. The foraging behavior of honey bees, Apis mellifera: a review. Veterinarni Medicina. 2014;59:1-10. https://doi.org/10.17221/7240-VETMED
  2. Alaux C, Ducloz F, Crauser D, Conte YL. Diet effects on honeybee immunocompetence. Biol Lett. 2010;6:562-5 https://doi.org/10.1098/rsbl.2009.0986.
  3. AOAC. AOAC Official methods of analysis, 15th edition. 1990.
  4. Behmer ST. Insect herbivore nutrient regulation. Annu Rev Entomol. 2009;54:165-87. https://doi.org/10.1146/annurev.ento.54.110807.090537
  5. Bilisik A, Cakmak I, Bicakci A, Malyer H. Seasonal variation of collected pollen loads of honeybees (Apis mellifera L. anatoliaca). Grana. 2008;47:70-77. https://doi.org/10.1080/00173130801923976
  6. Brunet J, Thairu MW, Henss JM, Link RI, Kluevert JA. The effects of flower, floral display, and reward sizes on bumblebee foraging behaviour when pollen is the reward and plants are dichogamous. Int J Plant Sci. 2015; https://doi.org/10.1086/683339.
  7. Camazine S. The regulation of pollen foraging by honey bees: how foragers assess the colony’s need for pollen. Bahav Ecol Sociobiol. 1993;32:265-72.
  8. Cooper PD, Schaffer WM, Buchman SL. Temperature regulation of honeybees (Apis mellifera) foraging in the Sonoran desert. J. Exp. Biol. 1985;114:1-15.
  9. De Groot AP. Protein and amino acid requirements of the honeybee (Apis mellifera L.). Physiol Comp Ecol. 1953;3:197-285.
  10. Donkersley P, Phodes G, Pickup RW, Jones KC, Power EF, Wright GA, Wilson K. Nutritional composition of honey bee food stores vary with floral composition. Oecologia. 2017; https://doi.org/10.1007/s00442-017-3968-3.
  11. Fahrenholz L, Lamprecht IL, Schricker B. Thermal investigations of a honey bee colony: thermoregulation of the hive during summer and winter and heat production of members of different bee castes. J Comp Physiol B. 1989;159:551-60. https://doi.org/10.1007/BF00694379
  12. Ghosh S, Jung C. Nutritional value of bee-collected pollens of hardy kiwi, Actinidia arguta (Actinidiaceae) and oak, Quercus sp. (Fagaceae). J Asia-Pac Entomol. 2017;20:245-51. https://doi.org/10.1016/j.aspen.2017.01.009
  13. Hanley ME, Franco M, Pichon S, Darvill B, Goulson D. Breeding system, pollinator choice and variation in pollen quality in Brittish herbaceous plants. Funct Ecol. 2008;22:592-8. https://doi.org/10.1111/j.1365-2435.2008.01415.x
  14. Hӧlldobler B, Wilson EO. The superorganism: the beauty, elegance, and strangeness of insect societies. New York: Norton; 2009.
  15. Human H, Nicolson SW. Nutritional content of fresh, bee-collected and stored pollen of Aloe greatheadii var. davyana (Asphodelaceae). Phytochem. 2006;67:1486-92. https://doi.org/10.1016/j.phytochem.2006.05.023
  16. Jeong SM, Lee CY, Kim DW, Jung C. Questionnaire study on the overwintering success and pest management of honeybee and damage assessment of Vespa hornets in Korea. J Apic. 2016;31:201-10. https://doi.org/10.17519/apiculture.2016.09.31.3.201
  17. Jung C, Burgett M. Effect of synthetic brood pheromone treatment on foraging benhaviour of the European honeybee, Apis mellifera L. Kor J Apic. 2011;26:255-60.
  18. Jung C, Choi SH, Kim YI. Determination of synthetic honeybee brood pheromone and its effect on honeybee colony Apis mellifera L.: preliminary study. Kor J Apic. 2011;26:249-54.
  19. Kitaoka TK, Nieh JC. Bumble bee pollen foraging regulation: role of pollen quality, storage levels, and odor. Behav Ecol Sociobiol. 2009;63:501-10. https://doi.org/10.1007/s00265-008-0684-3
  20. Klein S, Pasquaretta C, He XJ, Perry C, Sovik E, Devaud JM, Barron AB, Lihoreau M. Honey bee increase their foraging performance and frequency of pollen trips through experience. Sci Rep. 2019; https://doi.org/10.1038/s41598-019-42677-x.
  21. Kratochwil A, Kohl A, Br F. Pollensammel-Praferenzen bei Hummeln-ein Vergleich mit der Honigbiene, Mittelbadischer Landseverb. Mitt. bad. Landesver. Naturkunde u. Naturschutz. 1988;3:697-715.
  22. Lee KP, Behmer ST, Simpson SJ. Nutrient regulation in relation to diet breadth: a composition of Heliothis sister species and a hybrid. J Exp Biol. 2006; https://doi.org/10.1242/jeb.02253.
  23. Leonhardt SD, Bluthgen N. 2012. The same, but different: pollen foraging in honeybee and bumblebee colonies. Apidologie. 2012;43:449-64. https://doi.org/10.1007/s13592-011-0112-y
  24. McCaughey WF, Gilliam M, Standifer LN. Amino acids and protein adequacy for honey bees of pollens from desert plants and other floral sources. Apidologie. 1980;11:75-86. https://doi.org/10.1051/apido:19800109
  25. Moerman R, Vanderplanck M, Roger N, Decleves S, Wathelet B, Rasmont P, Fournier D, Michez D. Growth rate of Bumblebee larvae is related to pollen amino acids. J Econ Entomol. 2016; https://doi.org/10.1093/jee/tov279.
  26. Mollaei M, Hoseini SA, Karimi M, Hekmat Z. Impact of the amino acid proline on the cold hardiness of honey bee, Apis mellifera L. Span J Agric Res. 2013;11:714-7 https://doi.org/10.5424/sjar/2013113-3842.
  27. Negri P, Ramirez L, Quintana S, Szawarski N, Maggi M, Conte YL, Lamattina L, Eguaras M. Dietary supplementation of honey bee larvae with arginine and abscisic acid enhances nitric oxide and granulocyte immune responses after trauma. Insects. 2017;8:85 https://doi.org/10.3390/insects8030085.
  28. Pankiw T, Page RE Jr, Fondrk MK. Brood pheromone stimulates pollen foraging in honeybees (Apis mellifera). Bahav Ecol Sociobiol. 1998;44:193-8. https://doi.org/10.1007/s002650050531
  29. Paoli PP, Donley D, Stabler D, Saseendranath S, Nicolson SW, Simpson SJ, Wright GA. Nutritional balance of essential amino acids and carbohydrates of the adult worker honeybee depends on age. Amino Acids. 2014;46:1449-58. https://doi.org/10.1007/s00726-014-1706-2
  30. Pernal SF, Currie RW. The influence of pollen quality on foraging behaviour in honeybees (Apis mellifera L.). Behav Ecol Sociobiol. 2010;51:53-68. https://doi.org/10.1007/s002650100412
  31. Robertson AW, Mountjoy C, Faulkner BE, Roberts MV, Macnair MR. Bumble bee selection of Mimulus guttatus flowers: the effects of pollen quality and reward depletion. Ecol. 1999;80:2594-606. https://doi.org/10.1890/0012-9658(1999)080[2594:BBSOMG]2.0.CO;2
  32. Rogala R, Szymas B. Nutritional value for bees of pollen substitute enriched with synthetic amino acids, Part II. Biological Methods. J Apic Sci. 2004;48:29-36.
  33. Roulston TH, Cane JH, Buchmann SL. What governs protein content of pollen: pollinator preferences, pollen-pistil interactions, or phylogeny? Ecol Monogr. 2000;70:617-43. https://doi.org/10.1890/0012-9615(2000)070[0617:WGPCOP]2.0.CO;2
  34. Seeley TD. Honey Bee Ecology. Princeton: Princeton University Press; 1985.
  35. Van der Moezel PG, Delfs JC, Pate JS, Loneragan WA, Bell DT. 1987. Pollen selection by honeybees in Shrublands of the Northern Sandplains of Western Australia. J Apic Res. 1987;26:224-32. https://doi.org/10.1080/00218839.1987.11100764
  36. Vanderplanck M, Moerman R, Rasmont P, Lognay G, Wathelet B, Wattiez R, Michez D. How does pollen chemistry impact development and feeding behaviour of polylectic bees? PLoS ONE. 2014;9:e86209 https://doi.org/10.1371/journal.pone.0086209.
  37. Vaudo AD, Patch HM, Mortensen DA, Tooker JF, Grozinger CM. Macronutrient ratios in pollen shape bumblebee (Bombus impatiens) foraging strategies and floral preference. Proc Nat Acad Sci. 2016;https://doi.org/10.1073/pnas.1606101113.
  38. Willmer P. Pollination and floral ecology: Princeton University Press; 2011.
  39. Winston M. The biology of the honeybee: Harvard University Press; 1987.
  40. Yang K, Wu D, Ye X, Liu D, Chen J, Sun P. Characterization of chemical composition of bee pollen in China. J Agric Food Chem. 2013;61:708-18 https://doi.org/10.1021/jf304056b.
  41. Yi HB, Jung C. Colony temperature regulation by the European Henybee (Apis mellifera L.) in late summer in temperate region. Kor J Apic. 2010;25:1-7.
  42. Yucel B, Duman I. Effect of foraging activity of honeybees (Apis mellifera L.) on onion (Allium cepa) seed production and quality. Pak J Biol Sci. 2005;8:123-6. https://doi.org/10.3923/pjbs.2005.123.126

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