Natural Product Sciences

  • 제26권1호
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  • Pages.71-78
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  • 2020
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
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Dendropanax morbifera Extract Inhibits Intimal Hyperplasia in Balloon-Injured Rat Carotid Arteries by Modulating Phenotypic Changes in Vascular Smooth Muscle Cells

  • Lim, Leejin (Cancer Mutation Research Center, Chosun University) ;
  • Jo, Juyeong (Department of Biomedical Sciences, Chosun University Graduate School) ;
  • Yoon, Sang Pil (Department of Anatomy, School of Medicine, Jeju National University) ;
  • Jang, Inyoub (Department of Anatomy, Chosun University School of Medicine) ;
  • Ki, Young-Jae (Department of Internal Medicine, Chosun University School of Medicine) ;
  • Choi, Dong-Hyun (Department of Internal Medicine, Chosun University School of Medicine) ;
  • Song, Heesang (Department of Biomedical Sciences, Chosun University Graduate School)
  • 투고 : 2019.11.05
  • 심사 : 2020.03.19
  • 발행 : 2020.03.31
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초록

The plant Dendropanax morbifera Léveille is effective folk medicines for the treatment of several conditions, such as infectious diseases, skin diseases, and other illnesses. Although the inhibitory effects of D. morbifera on the proliferation and migration of vascular smooth muscle cells (VSMCs) have been shown in our previous study, its effects in vivo remain to be elucidated. In this study, we aimed to investigate the protective effects of the extracts from D. morbifera (EDM) on neointimal hyperplasia of rat carotid artery and explore the underlying mechanisms. We observed that the ratio of intima to media thickness (I/M) was significantly decreased in the EDM-treated groups by ~80% compared to that of the control. The expression of Ki-67 and proliferating cell nuclear antigen was decreased by ~70% in the EDM-treated groups compared to that of the control. In addition, matrix metalloproteinase (MMP)2 and MMP9 significantly reduced in the neointimal layer of the EDM-treated groups. Moreover, the decreased levels of contractile phenotypic markers of VSMCs, such as α-smooth muscle actin, myocardin, and smooth muscle-myosin heavy chain, were successfully restored by EDM treatment. Furthermore, the levels of synthetic phenotypic markers, cellular retinal binding protein 1 and connexin 43 were also restored to normal levels. These results suggest that EDM inhibits vascular neointimal hyperplasia induced by balloon injury in rats via phenotypic modulation of VSMCs. Therefore, EDM may be a potential drug candidate for the prevention of restenosis.

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참고문헌

  1. Ross, R. N. Engl. J. Med. 1999, 340, 115-126. https://doi.org/10.1056/NEJM199901143400207
  2. Schwartz, S. M. J. Clin. Invest. 1997,100, S87- S89.
  3. Karim, M. A.; Miller, D. D.; Farrar, M. A.; Eleftheriades, E.; Reddy, B. H.; Breland, C. M.; Samarel, A. M. Circulation 1995, 91, 2049-2057. https://doi.org/10.1161/01.CIR.91.7.2049
  4. Labinaz, M.; Pels, K.; Hoffert, C.; Aggarwal, S.; O'Brien, E. R. Cardiovasc. Res. 1999, 41, 255-266. https://doi.org/10.1016/S0008-6363(98)00203-X
  5. Ip, J. H.; Fuster, V.; Israel, D.; Badimon, L.; Badimon, J.; Chesebro, J. H. J. Am. Coll. Cardiol. 1991, 17, 77B-88B.
  6. Clowes, A. W.; Reidy, M. A.; Clowes, M. M. Lab. invest. 1983, 49, 208-215.
  7. Braun-Dullaeus, R. C.; Mann, M. J.; Dzau, V. J. Circulation. 1998, 98, 82-89. https://doi.org/10.1161/01.CIR.98.1.82
  8. Owens, G. K.; Kumar, M. S.; Wamhoff, B. R. Physiol. Rev. 2004, 84, 767-801. https://doi.org/10.1152/physrev.00041.2003
  9. Li, Y. H.; Chung, H. C.; Liu, S. L.; Chao, T. H.; Chen, J. C. Int. Heart J. 2009, 50, 207-220. https://doi.org/10.1536/ihj.50.207
  10. Zhang, S.; Deng, J.; Gao, Y.; Yang, D. L.; Gong, Q. H.; Huang, X. N. Eur. J. Pharmacol. 2012, 685, 126-132. https://doi.org/10.1016/j.ejphar.2012.04.032
  11. Yu, H. Y.; Kim, K. S.; Lee, Y. C.; Moon, H. I.; Lee, J. H. Evid. Based Complement. Alternat. Med. 2012, 2012, 637512.
  12. Park, B. Y.; Min, B. S.; Oh, S. R.; Kim, J. H.; Kim, T. J.; Kim, D. H.; Bae, K. H.; Lee, H. K. J. Ethnopharmacol. 2004, 90, 403-408. https://doi.org/10.1016/j.jep.2003.11.002
  13. Moon, H. I. Hum. Exp. Toxicol. 2011, 30, 870-875. https://doi.org/10.1177/0960327110382131
  14. Chung, I. M.; Kim, M. Y.; Park, W. H.; Moon, H. I. Pharmazie 2009, 64, 547-549.
  15. Chung, I. M.; Kim, M. Y.; Park, S. D.; Park, W. H.; Moon, H. I. Phytother. Res. 2009, 23, 1634-1637. https://doi.org/10.1002/ptr.2838
  16. Chung, I. M.; Song, H. K.; Kim, S. J.; Moon, H. I. Phytother. Res. 2011, 25, 784-786. https://doi.org/10.1002/ptr.3336
  17. Richmond, J. D.; Agius, B. R.; Wright, B. S.; Haber, W. A.; Moriarity, D. M.; Setzer, W. N. Nat. Prod. Commun. 2009, 4, 271-274.
  18. Yu, H. Y.; Jin, C. Y.; Kim, K. S.; Lee, Y.C.; Park, S. H.; Kim, G. Y.; Kim, W. J.; Moon, H. I.; Choi, Y. H.; Lee, J. H. J. Agric. Food Chem. 2012, 60, 5400-5406. https://doi.org/10.1021/jf3014475
  19. Lim, L.; Yun, J. J.; Jeong, J. E.; Wi, A. J.; Song, H. J. Nanosci. Nanotechnol. 2015, 15, 116-119. https://doi.org/10.1166/jnn.2015.8382
  20. Lim, L.; Ju, S.; Song, H. Nat. Prod. Sci. 2019, 25, 136-142. https://doi.org/10.20307/nps.2019.25.2.136
  21. Kurki, P.; Ogata, K.; Tan, E. M. J. Immunol. Methods. 1988, 109, 49-59. https://doi.org/10.1016/0022-1759(88)90441-3
  22. Hilker, M.; Tellmann, G.; Buerke, M.; Gloger, K.; Moersig, W.; Oelert, H.; Hake, U.; Lehr, H. A. Cardiovasc. Pathol. 2002, 11, 284-290. https://doi.org/10.1016/S1054-8807(02)00113-8
  23. Song, H.; Li, Y.; Lee, J.; Schwartz, A. L.; Bu, G. Cancer Res. 2009, 69, 879-886. https://doi.org/10.1158/0008-5472.CAN-08-3379
  24. Newby, A. C. Cardiovasc. Res. 2006, 69, 614-624. https://doi.org/10.1016/j.cardiores.2005.08.002
  25. Dey, N. B.; Lincoln, T. M. Mol. Cell. Biochem. 2012, 368, 27-35. https://doi.org/10.1007/s11010-012-1339-2
  26. Chien, Y. C.; Huang, G. J.; Cheng, H. C.; Wu, C. H.; Sheu, M. J. J. Nat. Prod. 2012, 75, 1524-1533. https://doi.org/10.1021/np3002145
  27. Valente, A. J.; Yoshida, T.; Murthy, S. N.; Sakamuri, S. S.; Katsuyama, M.; Clark, R. A.; Delafontaine, P.; Chandrasekar, B. Am. J. Physiol. Heart Circ. Physiol. 2012, 303, H282-H296. https://doi.org/10.1152/ajpheart.00231.2012
  28. Thomas, J. A.; Deaton, R. A.; Hastings, N. E.; Shang, Y.; Moehle, C. W.; Eriksson, U.; Topouzis, S.; Wamhoff, B. R.; Blackman, B. R.; Owens, G. K. Am. J. Physiol. Heart Circ. Physiol. 2009, 296, H442-H452. https://doi.org/10.1152/ajpheart.00165.2008
  29. Amin, A. R.; Kucuk, O.; Khuri, F. R.; Shin, D. M. J. Clin. Oncol. 2009, 27, 2712-2725. https://doi.org/10.1200/JCO.2008.20.6235
  30. Jiang, F.; Dusting, G. J. Curr. Vasc. Pharmacol. 2003, 1, 135-156. https://doi.org/10.2174/1570161033476736
  31. Xu, K.; Al-Ani, M. K.; Pan, X.; Chi, Q.; Dong, N.; Qiu, X. Evid. Based Complement. Alternat. Med. 2018, 2018, 3549312.
  32. Kim, W.; Kim, D. W.; Yoo, D. Y.; Jung, H. Y.; Nam, S. M.; Kim, J. W.; Hong, S. M.; Kim, D. W.; Choi, J. H.; Moon, S. M.; Yoon, Y. S.; Hwang, I. K. BMC Complement. Altern. Med. 2014, 14, 428. https://doi.org/10.1186/1472-6882-14-428
  33. Lee, J. W.; Park, C.; Han, M. H.; Hong, S. H.; Lee, T. K.; Lee, S. H.; Kim, G. Y.; Choi, Y. H. Oncol. Rep. 2013, 30, 1231-1238. https://doi.org/10.3892/or.2013.2542
  34. Rensen, S. S. M.; Doevendans, P. A. F. M.; van Eys, G. J. J. M. Neth. Heart J. 2007, 15, 100-108. https://doi.org/10.1007/BF03085963