Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Morphological and Molecular Diagnosis of Necator americanus and Ancylostoma ceylanicum Recovered from Villagers in Northern Cambodia

  • Chang, Taehee (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Jung, Bong-Kwang (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Hong, Sooji (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Shin, Hyejoo (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Ryoo, Seungwan (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Jeonggyu (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Keon Hoon (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Khieu, Virak (National Center for Parasitology, Entomology and Malaria Control, Ministry of Health) ;
  • Huy, Rekol (National Center for Parasitology, Entomology and Malaria Control, Ministry of Health) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion)
  • Received : 2020.10.03
  • Accepted : 2020.11.03
  • Published : 2020.12.31
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Abstract

Human hookworm infections caused by adult Ancylostoma spp. and Necator americanus are one of the most important tropical diseases. We performed a survey of intestinal helminths using the Kato-Katz fecal examination technique targeting 1,156 villagers residing in 2 northern provinces (Preah Vihear and Stung Treng) of Cambodia in 2018. The results revealed a high overall egg positive rate of intestinal helminths (61.9%), and the egg positive rate of hookworms was 11.6%. Nine of the hookworm egg positive cases in Preah Vihear Province were treated with 5-10 mg/kg pyrantel pamoate followed by purging with magnesium salts, and a total of 65 adult hookworms were expelled in diarrheic stools. The adult hookworms were analyzed morphologically and molecularly to confirm the species. The morphologies of the buccal cavity and dorsal rays on the costa were observed with a light microscope, and the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were analyzed. The majority of the hookworm adults (90.7%) were N. americanus, whereas the remaining 9.3% were Ancylostoma ceylanicum, a rare hookworm species infecting humans. The results revealed a high prevalence of hookworm infections among people in a northern part of Cambodia, suggesting the necessity of a sustained survey combined with control measures against hookworm infections.

Keywords

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