Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Production of a transgenic pig expressing 3D8 single chain variable fragment (scFv) and its evaluation of PRRS resistant

3D8 scFv 형질전환 돼지 개발 및 PRRS 저항성 평가

  • Lee, Hwi-Cheul (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Gunsup (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Ji-Yoon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Bo Ram (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Mi-Ryung (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Poongyeon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Byun, Sung-June (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Won-Il (College of Veterinary Medicine, Chonbuk National University) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이건섭 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 김지윤 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 양현 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이보람 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 박미령 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황인설 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이풍연 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 변승준 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 김원일 (전북대학교 수의과대학) ;
  • 오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2020.11.08
  • Accepted : 2020.12.09
  • Published : 2020.12.30
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Abstract

In this study, we have developed 3D8 scFv transgenic pig (TG) by microinjection of fertilized one-cell pig zygotes (2.17%). The effect of 3D8 scFv TG on porcine reproductive and respiratory syndrome virus (PRRSV) resistance were evaluated through PRRSV VR2332 (1×103 TCID50/mL) challenge and transmission experiments. As a result, the average daily weight gain (ADWG) of TG increased compared to the wild type pigs (WT) in PRRSV challenge groups and the serum viremia levels of the TG was significantly lower than of WT on the 7 day and 21 day after infection, meaning that the viral shedding was suppressed by 3D8 scFv expression. These results suggest that the expression of 3D8 scFv in pig could suppress spreading of infected virus to pigs sharing a room.

Keywords

References

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