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Neofusicoccum mangiferae and Thyronectria cucurbitula: Unrecorded Endophytic Fungi Isolated from the Leaves of Woody Plants in Korea

목본식물의 잎에서 분리된 국내 미기록 내생균: Neofusicoccum mangiferae and Thyronectria cucurbitula

  • Lee, Jong-Chul (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 이종철 (한국교원대학교 생물교육과) ;
  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2020.11.06
  • Accepted : 2020.12.09
  • Published : 2020.12.31

Abstract

We isolated endophytic fungi from the leaves of woody plants inhabiting the Ulleungdo Island and Yeosu in South Korea. The fungal strains were identified based on their morphological characteristics in conjunction with phylogenetic analysis, which was performed using sequences of the internal transcribed spacer, large subunit rDNA, and β-tubulin DNA. We confirmed the presence of Neofusicoccum mangiferae and Thyronectria cucurbitula, two endophytic fungal species that had not been previously recorded in Korea. In this report, we describe the morphological characteristics of these fungal strains and results of the DNA-based phylogenetic analysis.

본 연구에서는 울릉도 및 여수에 서식하는 목본식물의 잎을 채취하고 내생균을 분리하였다. 분리된 균주는 배양 특성과 분생포자 등의 형태성 및 rDNA의 internal transcribed spacer 영역, large subunit 영역, 그리고 β-tubulin 영역의 DNA 염기서열을 분석하여 동정하였다. 연구 결과 국내 미기록 내생균 2종을 확인하였으며, 확인된 종은 Neofusicoccum mangiferae과 Thyronectria cucurbitula이다. 확인된 미기록종 균주의 형태적 특성 및 분자생물학적 계통분석의 결과에 대해 서술하였다.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the ministry of Environment (MOE) of the Republic of Korea (NIBR201902202).

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