Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Verification of the Antioxidant Effects and Whitening Activity of fermented Ambrosia trifida L. Extracts in B16F10 Cells

단풍잎돼지풀 발효 추출물의 항산화 효과 및 B16F10 세포에서의 미백 활성 검증

  • Yoo, Dan-Hee (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Oh, Min-Jeong (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Yeom, Hyeon-Ji (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Lee, Jin-Young (Division of Cosmetics Biotechnology, Hoseo University)
  • 유단희 (호서대학교 화장품생명공학부) ;
  • 오민정 (호서대학교 화장품생명공학부) ;
  • 염현지 (호서대학교 화장품생명공학부) ;
  • 이진영 (호서대학교 화장품생명공학부)
  • Received : 2020.06.09
  • Accepted : 2020.09.07
  • Published : 2020.12.28
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Abstract

The purpose of this study was to verify the antioxidant and whitening effects of fermented Ambrosia trifida L. extract (ATFE) and to verify its usefulness as a cosmetic material. The antioxidant effects were measured by assessing the electron-donating capacity and 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability of these extracts. ATFE was shown to have an electron-donation capacity of 68.4% at a concentration of 1000 ㎍/ml. While its ABTS+ radical scavenging ability was shown to be 58.7% at the same concentration. The ATFE tyrosinase inhibitory effect, which is related to skin-whitening, was shown to be 32.35% at a concentration of 1000 ㎍/ml and a cell viability assay using melanoma cells showed a 14.8% reduction in cell viability at a concentration of 100 ㎍/ml. Surviving cells were then used in western blot analyses to evaluate the protein inhibitory effects of ATFE at 25, 50, 100 ㎍/ml where β-actin was used as a positive control. The whitening effects of these extracts were also evaluated by western blot and show that the expression of microphthalmia-associated transcription factors, Tyrosinase-related proteins (TRP)-1, TRP-2 and Tyrosinase were all inhibited, 51.14%, 55.4%, 38.6%, 83.77% respectively, at 100 ㎍/ml ATFE. The efficacy of the whitening effects was verified and the suitability of ATFE as a cosmetic material was assured.

본 연구는 단풍잎돼지풀 발효 추출물의 항산화 및 미백 효과를 검증하여 화장품 소재로서 활용가능성을 확인하고자 하였다. 항산화 효과를 측정하기 위해 전자공여능 측정과 ABTS 라디칼 소거능을 측정하였다. 단풍잎돼지풀 발효 추출물의 전자공여능 측정 결과 1,000 ㎍/ml 농도에서 68.4%의 효과를 보였으며, ABTS 라디칼 소거능 측정 결과 같은 농도에서 58.7%의 효과를 나타내었다. 미백효과를 Tyrosinase의 효소 억제 활성에 의해 측정한 결과, 1,000 ㎍/ml 농도에서 32.4%의 저해활성 효과를 보였다. 세포 차원에서 미백효과를 측정하기 위해 단풍잎돼지풀 발효추출물의 세포 생존율을 멜라노마 세포에서 측정하였다. 그 결과, 100 ㎍/ml 농도에서 85.2%의 생존율을 보였으며, 독성을 보이지 않는 농도인 100 ㎍/ml 농도 이하에서 western blot을 진행하였다. 단풍잎돼지풀 발효 추출물의 단백질 발현억제 효과를 25, 50, 100 ㎍/ml의 농도에서 western blot으로 측정하였으며, 양성대조군으로 β-actin을 사용하였다. 그 결과, 100 ㎍/ml 농도에서 MITF, TRP-1, TRP-2, Tyrosinase인자들은 각각 51.14%, 55.4%, 38.6%, 83.77%의 효과를 나타내었다. 결론적으로 단풍잎돼지풀 발효 추출물의 항산화 및 미백효과가 검증되었으며, 화장품 천연물 소재로서 활용가능성을 확인하였다.

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