한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제48권4호
  • /
  • Pages.556-563
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  • 2020
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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DOI QR Code

단풍잎돼지풀 발효 추출물의 항산화 효과 및 B16F10 세포에서의 미백 활성 검증

Verification of the Antioxidant Effects and Whitening Activity of fermented Ambrosia trifida L. Extracts in B16F10 Cells

  • 유단희 (호서대학교 화장품생명공학부) ;
  • 오민정 (호서대학교 화장품생명공학부) ;
  • 염현지 (호서대학교 화장품생명공학부) ;
  • 이진영 (호서대학교 화장품생명공학부)
  • Yoo, Dan-Hee (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Oh, Min-Jeong (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Yeom, Hyeon-Ji (Division of Cosmetics Biotechnology, Hoseo University) ;
  • Lee, Jin-Young (Division of Cosmetics Biotechnology, Hoseo University)
  • 투고 : 2020.06.09
  • 심사 : 2020.09.07
  • 발행 : 2020.12.28
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초록

본 연구는 단풍잎돼지풀 발효 추출물의 항산화 및 미백 효과를 검증하여 화장품 소재로서 활용가능성을 확인하고자 하였다. 항산화 효과를 측정하기 위해 전자공여능 측정과 ABTS 라디칼 소거능을 측정하였다. 단풍잎돼지풀 발효 추출물의 전자공여능 측정 결과 1,000 ㎍/ml 농도에서 68.4%의 효과를 보였으며, ABTS 라디칼 소거능 측정 결과 같은 농도에서 58.7%의 효과를 나타내었다. 미백효과를 Tyrosinase의 효소 억제 활성에 의해 측정한 결과, 1,000 ㎍/ml 농도에서 32.4%의 저해활성 효과를 보였다. 세포 차원에서 미백효과를 측정하기 위해 단풍잎돼지풀 발효추출물의 세포 생존율을 멜라노마 세포에서 측정하였다. 그 결과, 100 ㎍/ml 농도에서 85.2%의 생존율을 보였으며, 독성을 보이지 않는 농도인 100 ㎍/ml 농도 이하에서 western blot을 진행하였다. 단풍잎돼지풀 발효 추출물의 단백질 발현억제 효과를 25, 50, 100 ㎍/ml의 농도에서 western blot으로 측정하였으며, 양성대조군으로 β-actin을 사용하였다. 그 결과, 100 ㎍/ml 농도에서 MITF, TRP-1, TRP-2, Tyrosinase인자들은 각각 51.14%, 55.4%, 38.6%, 83.77%의 효과를 나타내었다. 결론적으로 단풍잎돼지풀 발효 추출물의 항산화 및 미백효과가 검증되었으며, 화장품 천연물 소재로서 활용가능성을 확인하였다.

The purpose of this study was to verify the antioxidant and whitening effects of fermented Ambrosia trifida L. extract (ATFE) and to verify its usefulness as a cosmetic material. The antioxidant effects were measured by assessing the electron-donating capacity and 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability of these extracts. ATFE was shown to have an electron-donation capacity of 68.4% at a concentration of 1000 ㎍/ml. While its ABTS+ radical scavenging ability was shown to be 58.7% at the same concentration. The ATFE tyrosinase inhibitory effect, which is related to skin-whitening, was shown to be 32.35% at a concentration of 1000 ㎍/ml and a cell viability assay using melanoma cells showed a 14.8% reduction in cell viability at a concentration of 100 ㎍/ml. Surviving cells were then used in western blot analyses to evaluate the protein inhibitory effects of ATFE at 25, 50, 100 ㎍/ml where β-actin was used as a positive control. The whitening effects of these extracts were also evaluated by western blot and show that the expression of microphthalmia-associated transcription factors, Tyrosinase-related proteins (TRP)-1, TRP-2 and Tyrosinase were all inhibited, 51.14%, 55.4%, 38.6%, 83.77% respectively, at 100 ㎍/ml ATFE. The efficacy of the whitening effects was verified and the suitability of ATFE as a cosmetic material was assured.

키워드

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