Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a New Cellulase-producing Marine Bacterium, Seonamhaeicola sp. S2-3

셀룰로스분해 신규 해양미생물 Seonamhaeicola sp. S2-3의 분리 및 동정

  • Kim, Da Som (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resource) ;
  • Chi, Won-Jae (Microorganism Resources Division, Biological Resources Research Department, National Institute of Biological Resource)
  • 김다솜 (국립생물자원관 생물자원연구부 미생물자원과) ;
  • 지원재 (국립생물자원관 생물자원연구부 미생물자원과)
  • Received : 2020.09.11
  • Accepted : 2020.10.23
  • Published : 2020.12.28
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Abstract

A cellulolytic bacterial strain, S2-3, was isolated from sea water collected in Jeju island, Republic of Korea. The strain was aerobic and gram negative, and formed yellow colored colonies on marine agar medium. S2-3 cells were long rod-shaped, 0.5 × 0.25 ㎛ (width x length) in size, and did not have flagella. The optimal growth conditions for S2-3 were 30-35℃ and pH 6.5-7.0. Analysis of the 16S rRNA gene sequence of S2-3 revealed that it had the highest identity with those of Seonamhaeicola algicola Gy8 (97.08%), Hyunsoonleella udonensis JG48 (95.01%), and Aestuariibaculum scopimerae I-15 (94.86%). In phylogenetic analysis, S2-3 formed the same clade as S. algicola Gy8, implying that S2-3 belongs to the genus Seonamhaeicola. The major fatty acids (>10%) comprised C15:1 iso G (22.29%), C15:0 iso (17.71%), C17:0 iso 3OH (16.06%), and C15:0 iso 3OH (10.7%), resulting in quite different ratio of the component from those of S. algicola Gy8. Moreover, its biochemical characteristics, including acid production and enzyme activities, were different from those of S. algicola Gy8. Therefore, putting all these results together, we concluded S2-3 is distinct species from S. algicola Gy8, and thus named it Seonamhaeicola sp. S2-3. In liquid culture, S2-3 produced extracellular cellulases that can hydrolyze cellulose or cellooligosaccharides into cellobiose, which is a good enzyme resource that deserves further research.

Keywords

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