한국버섯학회지 (Journal of Mushroom)

  • 제18권4호
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  • Pages.372-379
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  • 2020
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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반응표면분석법을 이용한 흰목이버섯의 기능성 성분 추출 조건 최적화

Optimization of extraction conditions for functional ingredients from Tremella fuciformis Berk. using response surface methodology

  • 홍민 ((재)춘천바이오산업진흥원) ;
  • 최다혜 ((재)춘천바이오산업진흥원) ;
  • 한준희 ((재)춘천바이오산업진흥원) ;
  • 권태형 ((재)춘천바이오산업진흥원) ;
  • 이선엽 ((재)춘천바이오산업진흥원) ;
  • 이용진 ((재)춘천바이오산업진흥원) ;
  • 유근형 ((재)춘천바이오산업진흥원)
  • Hong, Min (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Choi, Da-Hye (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Han, Joon-Hee (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Kwon, Tae-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Sun-Yeop (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Lee, Yong-Jin (Department of Research and Development, Chuncheon Bioindustry Foundation) ;
  • Yu, Keun-Hyung (Department of Research and Development, Chuncheon Bioindustry Foundation)
  • 투고 : 2020.07.29
  • 심사 : 2020.12.03
  • 발행 : 2020.12.31
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초록

본 연구에서 흰목이버섯의 추출조건별 추출수율과 기능성 성분의 함량을 비교하여 최적 추출조건을 분석하였으며, 모든 분석에서 R2이 0.9331~0.9462로 유의성을 보이는 것을 확인하였다. 각 독립변수에 따른 추출수율과 ergothioneine, 𝛽-glucan 성분을 분석한 결과 추출수율은 추출온도와 추출시료농도에 반비례했으며, 추출시간에는 큰 영향이 없었다. Ergothioneine 성분은 온도가 증가할수록 증가하며, 추출시간은 4.33 h가 가장 높았고 시료량에 큰 영향이 없었다. 𝛽-glucan 성분은 온도가 증가할수록 감소했으며, 추출시간에 큰 영향이 없었고 시료량이 21.2 mg/mL에서 가장 높았다. 모든 종속변수의 최대 독립변수는 온도 60℃, 추출시간 4.33 h, 추출시료농도 16.6 mg/mL에서 추출수율 24.9%, ergothioneine 성분함량 66.8 ug/g, 𝛽-glucan 성분함량 34.9 g/100 g으로 나타났다.

Snow fungus (Tremella fuciformis) with functional contents has satisfactory effects on various diseases, including atherosclerosis, high cholesterol, healthy skin, cancer, diabetes, and anti-inflammation. In this study, the extraction yield and functional contents (ergothioneine and 𝛽-glucan) of white jelly fungus were compared based on the extraction conditions using response surface methodology. Results revealed the extraction conditions for optimization of the dependent variables to be 60℃ and 4.33 h, when 16.6 mg/mL of sample concentration was used. Under these conditions, the extraction yield was 24.9%, including ergothioneine (66.8 ㎍/g) and 𝛽-glucan (34.9 g/100 g). These results can be useful in understanding the functional ingredients and mass extraction process in mushroom.

키워드

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