한국버섯학회지 (Journal of Mushroom)

  • 제18권4호
  • /
  • Pages.317-322
  • /
  • 2020
  • /
  • 1738-0294(pISSN)
  • /
  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
권호 표지
DOI QR코드

DOI QR Code

병재배용 느타리 품종 『흑타리』의 배양온도에 따른 미발이 관계 규명

Cause of undeveloped primordium formation according to incubation temperature of new oyster mushroom cultivar 『Heuktari』 for bottle cultivation

  • 최종인 (경기도농업기술원 버섯연구소) ;
  • 김정한 (경기도농업기술원 버섯연구소) ;
  • 권희민 (경기도농업기술원 버섯연구소) ;
  • 이윤혜 (경기도농업기술원 버섯연구소) ;
  • 신복음 (경기도농업기술원 버섯연구소) ;
  • 구옥 (경기도농업기술원 버섯연구소) ;
  • 하태문 (경기도농업기술원 버섯연구소) ;
  • 정구현 (경기도농업기술원 버섯연구소)
  • Choi, Jong In (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Kim, Jeong Han (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Gwon, Hee Min (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Lee, Yun Hae (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Shin, Bok Eum (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Gu, Ok (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Ha, Tai Moon (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Jung, Gu Hyun (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
  • 투고 : 2020.12.06
  • 심사 : 2020.12.21
  • 발행 : 2020.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

느타리 '흑타리' 품종의 배양 중 고온스트레스에 의해 발생되는 미발이 현상을 구명하기 위하여 배양온도에 따른 생육차이를 조사하였다. PDA 배지에서 '흑타리'의 적정생육온도는 23~26℃였고, 균사생장속도는 '춘추2호'에 비하여 빠른편이었다. 병내 배지온도는 초기에 상승하여 배양 중반에 최고점에 도달한 후 온도가 하강하였다. 배양 온도가 높을수록 배양기간은 짧아졌다. 배양온도 20℃ 처리구에서 배양기간은 25일 정도 소요되었으며, 미발이율은 1.8%, 수량은 139.4 g/병을 나타내었다. 배양온도 24℃ 처리구에서 배양기간은 20일 정도 소요되었으며, 미발이율은 4.2%, 병당수량은 132.1 g/병을 나타내었다. 배양온도 16℃와 28℃처리구에서는 미발이율이 증가되었고 수량이 감소하였다. 이 결과를 바탕으로 농가에서 배양온도와 미발이율의 관계를 조사하였다. 배양실 온도를 18℃로 설정하고 배지품온을 28℃ 미만으로 관리하는 농가는 미발이율이 0.3~0.8%를 나타내었다. 배양실내의 온도가 20℃ 이상이며 환기가 잘 이루어지지 않은 농가에서는 미발이율이 3.5% 정도 발생되었다. 배양실 온도가 19℃이며 배지 최고 품온이 31.3℃까지 상승하는 농가는 미발이율이 8.2%로 높게 나타났다. 배양중 병내부 온도가 28℃이상 상승하고 배양실내의 환기가 잘 이루어지지 않을 경우 미발이율이 증가하고 수량이 감소되는 경향을 보였다. 결과적으로, 배양실은 배지품온이 28℃ 이상 상승하지 않도록 배양실 내부의 공기를 지속적으로 순환시키고, 배양공간에 맞는 최적의 배양량을 넣어 관리하여야 한다.

This experiment was conducted to solve the failure of fruiting body production in the bottle cultivation of the oyster mushroom cultivar 'Heuktari'. The effects of incubation temperature on primordium formation and fruiting body yield of the oyster mushroom cultivar 'Heuktari' were investigated. The proper temperature for mycelium growth of 'Heuktari' on potato dextrose agar (PDA) medium is 23-26℃. The mycelial growth of 'Heuktari' was faster than that of Chunchu 2ho. During mycelial culture in sawdust medium, the temperature of the medium in the bottle initially increased, reached the highest point in the middle of the culture, and then decreased. The higher the set temperature, the shorter the incubation period. When the incubation temperatures were 20℃ and 24℃, respectively, the undeveloped primordium formation rates were low (1.8% and 4.2%, respectively). However, the rate of undeveloped primordium formation increased, and the yield decreased at incubation temperatures of 16℃ and 28℃. Mushroom farms that set incubation temperatures to 18℃ and maintained the medium temperature at less than 28℃ showed undeveloped primordium formation rates ranging between 0.3-0.8%. The rate of undeveloped primordium formation increased and the yield decreased in the farms with high incubation temperatures (above 28℃). We found that in order to reduce undeveloped primordium formation, the air inside the incubation room should be circulated continuously so that the temperature of the medium does not rise above 28℃, and dense incubation conditions should be avoided.

키워드

참고문헌

  1. Ancin-Azpilicueta C, Barriuso-Esteban B, Nieto-Rojo R, Aristizabal-Lopez N. 2012. SO2 protects the amino nitrogen metabolism of Saccharomyces cerevisiae under thermal stress. Microb Biotechnol 5: 654-662. https://doi.org/10.1111/j.1751-7915.2012.00343.x
  2. Asthir B. 2015. Mechanisms of heat tolerance in crop plants. Biol Plant 59: 620-628. https://doi.org/10.1007/s10535-015-0539-5
  3. Chang S, Miles P. 2004. Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact, 2nd edition. CRC Press, Florida, USA. 315-324.
  4. Choi JI, Lee YH, Ha TM, Jeon DH, Chi JH, Shin PG. 2015. Characteristics of new mid-high temperature adaptable oyster mushroom variety "Heuktari" for bottle culture. J Mushrooms 13: 74-78. https://doi.org/10.14480/JM.2015.13.1.74
  5. Elbein AD, Pan YT, Pastuszak I, Carroll D. 2003. New insights on trehalose: A multifunctional molecule. Glycobiology 13: 17-27.
  6. Gancedo C, Flores CL. 2004. The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi. FEMS Yeast Res 4: 351-359. https://doi.org/10.1016/S1567-1356(03)00222-8
  7. Ha TM, Ji JH, Ju YC, Kim HD. 2003. Study on the characteristics of fruit body growth according to incubation temperatures and period for oyster mushroom. Mushroom Sci Prod 1(1): 34-43.
  8. Hou L, Wang L, Wu X, Gao W, Zhang J, Huang C. 2019. Expression patterns of two pal genes of Pleurotus ostreatus across developmental stages and under heat stress. BMC Microbiol 19(1): 231. https://doi.org/10.1186/s12866-019-1594-4
  9. Kong WW, Huang CY, Chen Q, Zou YJ, Zhao MR, Zhang JX. 2012a. Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis. Biotechnol Lett 34: 1915-1919. https://doi.org/10.1007/s10529-012-0988-2
  10. Kong WW, Huang CY, Chen Q, Zou YJ, Zhang JX. 2012b. Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis. Fungal Genet Biol 49: 15-20. https://doi.org/10.1016/j.fgb.2011.12.003
  11. Korea seed and variety service. 2006. Test guidelines for the protection of new varieties of plants (Pleurotus spp.).
  12. Lee SH, Yu BK, Kim HJ, Yun NK, Jung JC. 2015. Technology for improving the uniformity of the environment in the oyster mushroom cultivation house by using multi-layered shelves. Protect Horticul Plant Fact 24: 128-133. https://doi.org/10.12791/KSBEC.2015.24.2.128
  13. Ribeiro MJS, Leao LSC, Morais PB, Rosa CA, Panek AD. 1999. Trehalose accumulation by tropical yeast strains submitted to stress conditions. Antonie van Leeuwenhoek 75: 245-251. https://doi.org/10.1023/A:1001806012566
  14. Song C, Chen Q, Wu X, Zhang J, Huang C. 2014. Heat stress induces apoptotic-like cell death in two Pleurotus species. Curr Microbiol 69(5): 611-616. https://doi.org/10.1007/s00284-014-0634-4
  15. Wang GZ, Ma CJ, Luo Y, Zhou SS, Zhou Y, Ma XL, Cai YL, Yu HJ, Bian YB, Gong YH. 2018. Proteome and transcriptome reveal involvement of heat shock proteins and indoleacetic acid metabolism process in Lentinula edodes thermotolerance. Cell Physiol Biochem 50: 1617-1637. https://doi.org/10.1159/000494784
  16. Wang L, Wu X, Gao W, Zhao M, Zhang J, Huang C. 2017. Differential expression patterns of Pleurotus ostreatus catalase genes during developmental stages and under heat stress. Genes 8(11): 335. https://doi.org/10.3390/genes8110335
  17. Yoo YB, Oh MJ, Oh YL, Shin PG, Jang KY, Kong WS. 2016. Development trend of the mushroom industry. J Mushrooms 14(4): 142-154. https://doi.org/10.14480/JM.2016.14.4.142
  18. Yoon YC, Suh WM, Lee IB. 2006. Analysis of environment factors in Pleurotus eryngii cultivation house of permanent frame type structure. J Bio-Environ Control 15(2): 125-137.
  19. Zhang RY, Hu DD, Zhang YY, Goodwin PH, Huang CY, Chen Q, Gao W, Wu XL, Zou YJ, Qu JB. 2016a. Anoxia and anaerobic respiration are involved in "spawnburning" syndrome for edible mushroom Pleurotus eryngii grown at high temperatures. Sci Hortic 199: 75-80. https://doi.org/10.1016/j.scienta.2015.12.035
  20. Zhang X, Ren A, Li MJ, Cao PF, Chen TX, Zhang G, Shi L, Jiang AL, Zhao MW. 2016b. Heat stress modulates mycelium growth, heat shock protein expression, ganoderic acid biosynthesis, and hyphal branching of Ganoderma lucidum via cytosolic Ca2+. Appl Environ Microbiol 82: 4112-4125. https://doi.org/10.1128/AEM.01036-16