한국어병학회지 (Journal of fish pathology)

  • 제33권2호
  • /
  • Pages.153-161
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  • 2020
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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뱀장어(Anguilla japonica)에서 Cytochrome P450 1 gene 클로닝 및 benzo[a]pyrene 노출에 따른 발현 분석

Cytochrome P450 1 gene in Eel, Anguilla japonica: cloning and expression patterns after exposure to benzo[a]pyrene

  • 조현호 (부경대학교 대연캠퍼스 수산생명의학과) ;
  • 김주안 (부경대학교 대연캠퍼스 수산생명의학과) ;
  • 이승현 (부경대학교 대연캠퍼스 수산생명의학과) ;
  • 정준기 (부경대학교 대연캠퍼스 수산생명의학과)
  • Jo, Hyun Ho (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Ju An (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lee, Seung Hyun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Chung, Joon Ki (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2020.10.21
  • 심사 : 2020.12.04
  • 발행 : 2020.12.31
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초록

Cytochrome P450(CYP) gene is involved in the biotransformation of drugs and environmental pollutants. In this study, we analyzed the nucleotide sequence of the Anguilla japonica CYP1(AjCYP1) family gene and examined the relative expression of AjCYP1A, AjCYP1B and AjCYP1C1 in response to the exposure to environmental pollutants. After exposure to B[a]P 20mg/kg bw, the expression of AjCYP1 family gene increased over time. Among four tissues examined (liver, spleen, gill and kidney), AjCYP1 family gene was expressed significantly in the kidney. Compared with the control group, AjCYP1A was expressed about 5-fold at 48 hr, AjCYP1B about 6-fold at 24 hr, and AjCYP1C1 about 4-fold at 24 hr. However, after exposure to B[a]P 200mg/kg bw, AjCYP1A did not change in all tissues. On the other hand, AjCYP1B was expressed at about 4-fold at 24 hr in the spleen and 4-fold at 48 hr in the gill. Finally AjCYP1C1 was expressed 3.7-fold and 4.3-fold in the spleen and kidneys at 48 hr, respectively. Taken together, our results suggest that the expression of AjCYP1 gene in eel tissues might be used as a useful tool to assess the exposure to environmental pollutants in aquaculture system.

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참고문헌

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