Korean Chemical Engineering Research

  • 제58권1호
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  • Pages.29-35
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  • 2020
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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세포투과 펩티드를 이용한 주름개선 펩티드 GHKs의 피부흡수 증진

Enhancement of Skin Permeation of Anti-wrinkle Peptide GHKs Using Cell Penetrating Peptides

  • 박수인 (을지대학교 대학원 시니어헬스케어학과 화장품약리학 전공) ;
  • 안규민 (을지대학교 대학원 시니어헬스케어학과 화장품약리학 전공) ;
  • 김민기 (을지대학교 대학원 시니어헬스케어학과 화장품약리학 전공) ;
  • 허수현 (을지대학교 대학원 시니어헬스케어학과 화장품약리학 전공) ;
  • 신문삼 (을지대학교 대학원 시니어헬스케어학과 화장품약리학 전공)
  • Park, Su In (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University) ;
  • An, Gyu Min (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University) ;
  • Kim, Min Gi (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University) ;
  • Heo, Soo Hyeon (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University) ;
  • Shin, Moon Sam (Department of Senior Healthcare, majored in Cosmetic Pharmacology, Eulji University)
  • 투고 : 2019.07.08
  • 심사 : 2019.09.26
  • 발행 : 2020.02.01
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초록

본 연구에서는 경피흡수가 잘 안되는 주름개선 펩티드인 GHK, GHK-Cu, Pal-GHK에 대하여 세포투과 펩티드인 알르기닌 올리고머(tetra-D-arginine, R4)와 hexa-D-arginine, R6)를 첨가한 후 경피 투과도를 측정하여 그 결과를 다음 6가지 경우로 분석하였다. 첫번째로 주름개선 펩티드만 함유한 경우는 구리이온(Cu2+)과 팔미트산이 경피 투과율을 증진시키는 것을 알 수 있다. 두번째로, GHK에 알르기닌 올리고머(R4, R6)를 첨가한 경우는 알르기닌 올리고머(R4, R6)가 경피 투과율을 증가시켰으며, R4에서 더 좋은 경피 투과율 증가를 나타났다. 세번째로, GHK-Cu에 R4, R6를 첨가한 경우는 경피 투과율 증가가 나타났으며, R6 < R4 경피 투과율 순서로 나타났다. 네번째로 Pal-GHK에 R4, R6를 첨가한 경우에도 경피 투과율 증가가 나타났으며, R6 < R4 경피 투과율 순서로 나타났다. 다섯번 째로, R4를 GHK, GHK-Cu, Pal-GHK에 첨가한 경우에는 GHK+R4 < GHK-Cu+R4 < Pal-GHK+R4 순서로 경피 투과율 증가가 나타났다. 마지막으로 R6를 GHK, GHK-Cu, Pal-GHK에 첨가한 경우에는 GHK+R4 < GHK-Cu+R4 < Pal-GHK+R4 순서로 경피 투과율 증가가 나타났다. 이를 통하여 주름 개선 펩티드인 GHK, GHK-Cu, Pal-GHK의 피부 투과를 증가를 위한 최적의 조건을 제시하여 그 효능을 극대화할 수 있는 방안을 제시함으로써 주름 개선 기능성 화장품에서의 폭넓은 활용과 응용을 제안하고자 한다.

In this study, the skin permeability was measured by adding cell penetrating peptides, arginine oligomers; (tetra-D-arginine (R4) and hexa-D-arginine (R6)) to little skin-permeable anti-wrinkle peptides (GHK, GHK-Cu, and Pal-GHK), and the results were analyzed by the following six cases. First, in cases where only anti-wrinkle peptides were contained, copper ions (Cu2+) and palmitic acid enhanced the transdermal permeability. Second, when arginine oligomers (R4, R6) were added to GHK, arginine oligomers (R4, R6) increased percutaneous permeability, and R4 showed better percutaneous permeability. Third, the addition of R4 and R6 to GHK-Cu resulted in increased percutaneous transmittance, followed by R6 < R4 percutaneous transmittance. Fourth, when R4 and R6 were added to Pal-GHK, the percutaneous permeability increased with results in R6 < R4 order. Fifth, when R4 was added to GHK, GHK-Cu, and Pal-GHK, the transdermal permeability increased in the order of GHK+R4 < GHK-Cu+R4 < Pal-GHK+R4. Finally, the addition of R6 to GHK, GHK-Cu and Pal-GHK also resulted in increased percutaneous transmittance in the order of GHK+R4 < GHK-Cu+R4 < Pal-GHK+R4. This study provides optimal conditions for enhancing skin absorption of anti-wrinkle peptides GHK, GHK-Cu, and Pal-GHK, and propose a wide range of applications in anti-wrinkle functional cosmetics by suggesting ways to maximize their efficacy.

키워드

참고문헌

  1. Jung, D. Y., "Golyeongchinhwasaneob Sijanghyeonhwang Mich Jeonmang," Magazine IEEE, 44(12), 16-24(2017).
  2. Oh, J. S., "A Study on the Usage and Purchase Intention of Basic Cosmetics for Silver Generation Women," J. Cult. Exchange, 6(3), 77-97(2017).
  3. Kang, K. S., Kim, I. D., Kwon, R. H., Heo, Y. Y. Oh, S. H., Kim, M. A., Jung, H. J., Kang, H. Y. and Ha, B. J., "The Evaluation of Anti-wrinkle Effects in Oriental Herb Extract," J. Life Sci., 17(8), 1147-1151(2007). https://doi.org/10.5352/JLS.2007.17.8.1147
  4. Moh, S. H., Jung, D. H., Kim, H. S., Cho, M. J., Seo, H. H. and Kim, S. J., "Characteristics and Applications of Bioactive Peptides in Skin Care," Kor. Soc. Biotech. Bioeng., 26(6), 483-490(2011).
  5. Yoo, B. J. and Yeon, J. J., "Hwajangpum Wonlyoyong Pebtaideu-ui Hyomo Balhyo Saengsan Gisul," BT News, 24(2), 52-56(2017).
  6. Li, H., Low, Y. S. J., Chong, H. P., Zin, M. T., Lee, C. Y., Li, B., Leolukman M. and Kang L., "Microneedle-Mediated Delivery of Copper Peptide Through Skin," Pharmaceu. Res., 32(8), 2678-2689(2015). https://doi.org/10.1007/s11095-015-1652-z
  7. Maquart, F.X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J. C. and Borel, J. P., "Stimulation of Collagen Synthesis in Fibro-blast Cultures by the Tripeptide-copper Complex Glycyl-L-Histidyl- L-Lysine-$Cu_2^+$," FEBS Letters, 238(2), 343-346(1988). https://doi.org/10.1016/0014-5793(88)80509-X
  8. Pickart, L., "The Human Tri-peptide GHK and Tissue Remodeling," J. Biomat. Sci. Polym. Ed., 19(8), 969-988(2008). https://doi.org/10.1163/156856208784909435
  9. Maquart, F.X., Bellon, G., Chaqour, B., Wegrowski, J., Patt, L. M., Trachy, R. E., Monboisse J. C., Chastang F., Birembaut P. and Gillery P., "In Vivo Stimulation of Connective Tissue Accumulation by the Tripeptide-copper Complex Glycyl-L-Histidyl-L-Lysine-$Cu_2^+$ in Rat Experimental Wounds," J. Clinical Invest., 92(5), 2368-2376(1993). https://doi.org/10.1172/JCI116842
  10. Godet, D. and Marie, P. J., "Effects of the Tripeptide Glycyl-LHistidyl-L-Lysine Copper Complex on Osteoblastic Cell Spreading, Attachment and Phenotype," Cell. Mol. Biol., 41(8), 1081-1091(1995).
  11. Pickart, L., Freedman, J. H. and Loker W. J., "Growth-modulating Plasma Tripeptide may Function by Facilating Copper Uptake into Cells," Nature, 288(5792), 715-717(1980). https://doi.org/10.1038/288715a0
  12. Lee, H. J., Huang, Y. W., Chiou, S. H., Aronstam, R. S., "Polyhistidine Facilitates Direct Membrane Translocation of Cell-penetrating Peptides into Cells," Sci. Reports, 9(1), 93-98 (2019). https://doi.org/10.1038/s41598-018-36500-2
  13. Vive, E., Brodin, P. and Lebleu, B., "A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus," J. Biol. Chem., 272(25), 16010-16017(1997). https://doi.org/10.1074/jbc.272.25.16010
  14. Wang, K., Zhao, X., Yang, F., Liu, P. and Xing, J., "Percutaneous Delivery Application of Acylated Steric Acid-9-P(arginine) Cell Penetrating Peptides Used as Transdermal Penetration Enhancer," J. Biomed. Nanotech., 15(3), 417-430(2019). https://doi.org/10.1166/jbn.2019.2658
  15. Yi, D., Guoming, L., Gao, L. and Wei, L., "Interaction of Arginine Oligomer with Model Membrane," Biochem. Biophys. Res. Comm., 359(4), 1024-1029(2007). https://doi.org/10.1016/j.bbrc.2007.06.015
  16. Liang, W., Davalian, D. and Torchilin, V. P., "Interaction of a Novel Peptoid Enhancer-Arginine Oligomer with Bovine Submaxillary Mucin," Yao Xue Xue Bao, 39(12), 1011-1017(2004).
  17. Mazurowska, L. and Mojski, L. M., "Biological Activities of Selected Peptides: Skin Penetration Ability of Copper Complexes with Peptides," Int. J. Cosmet. Sci., 59(1), 59-69(2008).
  18. Kim, M. H., "Skin Barrier and Protein," J. Skin Barrier Res., 11(1), 28-34(2009).
  19. Franz, T. J., "Percutaneous Absorption. On the Relevance of in Vitro Data," J. Invest. Dermatol., 64(3), 190-195(1975). https://doi.org/10.1111/1523-1747.ep12533356
  20. Kim, E. N., Park, K. H., Kim, B. H. and Jeong, G. S., "Permeation Characteristics of Transdermal Preparations Containing Artemisiae Capillaris Herba in Franz Diffusion Cell," Kor. J. Pharma., 49(2), 165-171(2018).
  21. Kim, J. H., Yang, H. J., Won, B. R., Ahn, Y. J., Kang, M. K. and Park, S. N., "Preparation of Vitamin E Acetate Nano-emulsion and In Vitro Research Regarding Vitamin E Acetate Transdermal Delivery System Which Use Franz Diffusion Cell," J. Soc. Cosmet. Sci. Kor., 35(2), 91-101(2009).
  22. Jin, L. H., Bahn, J. H., Eum, W. S., Kwon, H. Y., Jang, S.H., Han, K. H., Kang, T. C., Won, M. H., Kang, J. H., Cho, S. W., Park, J. and Chio, S. Y., "Transduction of Human Catalase Mediated by an HIV-1 TAT Protein Basic Domain and Arginine-rich Peptides into Mammalian Cells, Free Radic," Biol. Med., 31, 1509-1519(2001).
  23. Rothbard, J. B., Garlington, S., Lin, Q., Kirschberg, T., Kreider, E., McGrane, P. L., Wender, P. A. and Khavari, P. A., "Conjugation of Arginine Oligomers to Cyclosporin A Facilitates Topical Delivery and Inhibition of Inflammation," Nat. Med., 6(11), 1253-1257 (2000). https://doi.org/10.1038/81359