Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Colorimetric Based Analysis Using Clustered Superparamagnetic Iron Oxide Nanoparticles for Glucose Detection

클러스터 초상자성체 산화철 나노입자를 이용한 색채학적 해석 기반 당 측정

  • Choi, Wonseok (Department of Biomedical Engineering, Yonsei University) ;
  • Key, Jaehong (Department of Biomedical Engineering, Yonsei University)
  • 최원석 (연세대학교 보건과학대학 의공학과) ;
  • 기재홍 (연세대학교 보건과학대학 의공학과)
  • Received : 2020.11.17
  • Accepted : 2020.12.18
  • Published : 2020.12.31
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Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are approved by the Food and Drug Administration (FDA) in the United States. SPIONs are used in magnetic resonance imaging (MRI) as contrast agents and targeted delivery in nanomedicine using external magnet sources. SPIONs act as an artificial peroxidase (i.e., nanozyme), and these reactions were highly stable in various pH conditions and temperatures. In this study, we report a nanozyme ability of the clustered SPIONs (CSPIONs) synthesized by the oil-in-water (O/W) method and coated with biocompatible poly(lactic-co-glycolic acid) (PLGA). We hypothesize that the CSPIONs can have high sensitivity toward H2O2 derived from the reaction between a fixed amount of glucose and glucose oxidase (GOX). As a result, CSPIONs oxidized a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) commonly used as a substrate for hydrogen peroxidase in the presence of H2O2, leading to a change in the color of the substrate. We also utilized a colorimetric assay at 417 nm using various glucose concentrations from 5 mM to 1.25 μM to validate β-D-glucose detection. This study demonstrated that the absorbance value increases along with increasing the glucose level. The results were highly repeated at concentrations below 5 mM (all standard deviations < 0.03). Moreover, the sensitivity and limit of detection were 1.50 and 5.44 μM, respectively, in which CSPIONs are more responsive to glucose than SPIONs. In conclusion, this study suggests that CSPIONs have the potential to be used for glucose detection in diabetic patients using a physiological fluid such as ocular, saliva, and urine.

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References

  1. Kandasamy Ganeshlenin, Maity Dipak. Recent advances in superparamagnetic iron oxide nanoparticles (SPIONs) for in vitro and in vivo cancer nanotheranostics. International journal of pharmaceutics. 2015;496(2):191-218. https://doi.org/10.1016/j.ijpharm.2015.10.058
  2. Dulinska-Litewka, Joanna, et al. Superparamagnetic iron oxide nanoparticles-Current and prospective medical applications. Materials. 2019;12(4):617. https://doi.org/10.3390/ma12040617
  3. Bae Ki Hyun, et al. Chitosan oligosaccharide-stabilized ferrimagnetic iron oxide nanocubes for magnetically modulated cancer hyperthermia. ACS nano. 2012;6(6):5266-73. https://doi.org/10.1021/nn301046w
  4. Kolen'ko, Yury V, et al. Large-scale synthesis of colloidal Fe3O4 nanoparticles exhibiting high heating efficiency in magnetic hyperthermia. The Journal of Physical Chemistry C. 2014; 118(16): 8691-701. https://doi.org/10.1021/jp500816u
  5. Nam Nguyen Hoai, et al. Folate attached, curcumin loaded Fe3O4 nanoparticles: A novel multifunctional drug delivery system for cancer treatment. Materials Chemistry and Physics. 2016;172:98-104. https://doi.org/10.1016/j.matchemphys.2015.12.065
  6. Li Li, et al. Superparamagnetic iron oxide nanoparticles as MRI contrast agents for non-invasive stem cell labeling and tracking. Theranostics. 2013;3(8):595. https://doi.org/10.7150/thno.5366
  7. Zhang Dianbao, et al. Polyethyleneimine-coated Fe3O4 nanoparticles for efficient siRNA delivery to human mesenchymal stem cells derived from different tissues. Science of Advanced Materials. 2015;7(6):1058-64. https://doi.org/10.1166/sam.2015.2178
  8. Gao Lizeng, et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nature nanotechnology. 2007;2(9):577-83. https://doi.org/10.1038/nnano.2007.260
  9. Yu Faquan, et al. The artificial peroxidase activity of magnetic iron oxide nanoparticles and its application to glucose detection. Biomaterials. 2009;30(27):4716-22. https://doi.org/10.1016/j.biomaterials.2009.05.005
  10. Yee Ying Chuin, et al. Colorimetric analysis of glucose oxidase-magnetic cellulose nanocrystals (CNCs) for glucose detection. Sensors. 2019;19(11):2511. https://doi.org/10.3390/s19112511
  11. Vallabani Nv Srikanth, Karakoti Ajay S, Singh Sanjay. ATPmediated intrinsic peroxidase-like activity of Fe3O4-based nanozyme: one step detection of blood glucose at physiological pH. Colloids and Surfaces B: Biointerfaces. 2017;153:52-60. https://doi.org/10.1016/j.colsurfb.2017.02.004
  12. Tanaka Shunsuke, et al. Mesoporous iron oxide synthesized using poly (styrene-b-acrylic acid-b-ethylene glycol) block copolymer micelles as templates for colorimetric and electrochemical detection of glucose. ACS applied materials & interfaces. 2018;10(1):1039-49. https://doi.org/10.1021/acsami.7b13835
  13. Jang Hongje, Min Dal-Hee. Highly precise plasmonic and colorimetric sensor based on enzymatic etching of nanospheres for the detection of blood and urinary glucose. RSC Advances. 2015;5(19):14330-2. https://doi.org/10.1039/C4RA15485A
  14. Dadfar Seyed Mohammadali, et al. Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications. Advanced drug delivery reviews. 2019;138:302-25. https://doi.org/10.1016/j.addr.2019.01.005
  15. Wang Manlin, et al. Fe3O4@ β-CD nanocomposite as heterogeneous Fenton-like catalyst for enhanced degradation of 4-chlorophenol (4-CP). Applied Catalysis B: Environmental. 2016;188(5):113-22. https://doi.org/10.1016/j.apcatb.2016.01.071
  16. Xu Lejin, Jianlong Wang. Fenton-like degradation of 2,4-dichlorophenol using Fe3O4 magnetic nanoparticles. Applied Catalysis B: Environmental. 2012;123:117-26. https://doi.org/10.1016/j.apcatb.2012.04.028
  17. Huang Xiaopeng, et al. Hematite facet confined ferrous ions as high efficient Fenton catalysts to degrade organic contaminants by lowering H2O2 decomposition energetic span. Applied Catalysis B: Environmental. 2016;181:127-37. https://doi.org/10.1016/j.apcatb.2015.06.061
  18. Greenwood, Norman Neill, Alan Earnshaw. Chemistry of the Elements. Elsevier, 2012.
  19. Makaram Prashanth, Owens Dawn, Aceros Juan. Trends in nanomaterial-based non-invasive diabetes sensing technologies. Diagnostics. 2014;4(2):27-46. https://doi.org/10.3390/diagnostics4020027
  20. Gupta Shruti, et al. Comparison of salivary and serum glucose levels in diabetic patients. Journal of diabetes science and technology. 2014;9(1):91-6. https://doi.org/10.1177/1932296814552673
  21. Hedayati Mohammad, et al. An optimised spectrophotometric assay for convenient and accurate quantitation of intracellular iron from iron oxide nanoparticles. International Journal of Hyperthermia. 2018;34(4):373-81. https://doi.org/10.1080/02656736.2017.1354403
  22. Hennessy Douglas J, et al. Ferene-a new spectrophotometric reagent for iron. Canadian journal of chemistry. 1984;62(4):721-4. https://doi.org/10.1139/v84-121
  23. Deda Daiana K, et al. A reliable protocol for colorimetric determination of iron oxide nanoparticle uptake by cells. Analytical and bioanalytical chemistry. 2017;409(28):6663-75. https://doi.org/10.1007/s00216-017-0622-1
  24. Walker HK, Hall WD, Hurst JW, Clinical methods: The history, physical, and laboratory examinations. 3rd edition. Boston: Butterworths; 1990.
  25. Liu Shanhu, et al. Structural effects of Fe3O4 nanocrystals on peroxidase-like activity. Chemistry-A European Journal. 2011;17(2): 620-5. https://doi.org/10.1002/chem.201001789
  26. Zhou Kebin, Li Yadong. Catalysis based on nanocrystals with well-defined facets. Angewandte Chemie International Edition. 2012;51(3):602-13. https://doi.org/10.1002/anie.201102619
  27. Bienert Gerd P, Jan K Schjoerring, Thomas P Jahn. Membrane transport of hydrogen peroxide. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2006;1758(8):994-1003. https://doi.org/10.1016/j.bbamem.2006.02.015
  28. Tang Jie, et al. Calcium phosphate embedded PLGA nanoparticles: A promising gene delivery vector with high gene loading and transfection efficiency. International journal of pharmaceutics. 2012;431(1-2): 210-21. https://doi.org/10.1016/j.ijpharm.2012.04.046
  29. Das Soumen, et al. Cerium oxide nanoparticles: applications and prospects in nanomedicine. Nanomedicine. 2013;8(9):1483-508. https://doi.org/10.2217/nnm.13.133
  30. Wu Yuao, et al. Novel iron oxide-cerium oxide core-shell nanoparticles as a potential theranostic material for ROS related inflammatory diseases. Journal of Materials Chemistry B. 2018;6(30):4937-51. https://doi.org/10.1039/C8TB00022K
  31. Mars P, Do W Van Krevelen. Oxidations carried out by means of vanadium oxide catalysts. Chemical Engineering Science. 1954;3:41-59. https://doi.org/10.1016/S0009-2509(54)80005-4
  32. Yu Kai, et al. Asymmetric Oxygen Vacancies: the Intrinsic Redox Active Sites in Metal Oxide Catalysts. Advanced Science. 2020;7(2):1901970. https://doi.org/10.1002/advs.201901970