Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans

Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과

  • Hwang, Hye-Rim (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kang, Si-Mook (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Lee, Eun-song (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kim, Baek-Il (Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry)
  • 황혜림 (연세대학교 치과대학 예방치과학교실, BK21 플러스 통합구강생명과학사업단) ;
  • 강시묵 (연세대학교 치과대학 예방치과학교실, BK21 플러스 통합구강생명과학사업단) ;
  • 이은송 (연세대학교 치과대학 예방치과학교실, BK21 플러스 통합구강생명과학사업단) ;
  • 김백일 (연세대학교 치과대학 예방치과학교실, BK21 플러스 통합구강생명과학사업단)
  • Received : 2019.05.20
  • Accepted : 2019.07.12
  • Published : 2019.09.30

Abstract

Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in $30m{\ell}$ of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. $1.8m{\ell}$ of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and $0.05mg/m{\ell}$, was mixed with $0.2m{\ell}$ of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with $0.05mg/m{\ell}$ curcuma powder extract, the concentration of $0.5mg/m{\ell}$ and LED light of 405 nm wavelength (p=0.000, p=0.003). $0.05mg/m{\ell}$ of curcuma powder extract and the concentration of $0.5mg/m{\ell}$ showed 100% antibacterial effect when exposed to light, whereas the concentration of $5mg/m{\ell}$ showed 11.95% antibacterial effect. When exposed to light, $0.05mg/m{\ell}$ of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of $0.5mg/m{\ell}$ and $5mg/m{\ell}$ did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.

Keywords

Acknowledgement

Supported by : 한국연구재단

References

  1. Adolfsson Erici M, Pettersson M, Paskkonen J, et al. Troclosan, a commonly used bactericide found in human milk and in the aquatic environment in Sweden. Chemosphere 2002;46(9-10):1485-1489 https://doi.org/10.1016/S0045-6535(01)00255-7
  2. 한국보건사회연구원. 항생제 내성. 글로벌 사회정책 브리프. Vol 20. 2016.
  3. Kim BI, Kim SN, Chang SY, et al. A highly selective antibacterial effect of Curcuma xanthorrhiza extract against oral pathogens and clinical effectiveness of a dentifrice containing Curcuma xanthorrhiza extract for controlling bad breath. J Korean Acad Dent Health 2005;9(2):222-237
  4. Aruoma OI, Spencer JP, Rossi R, et al. An evaluation of the antioxidant and antiviralaction of extracts of rosemary and Provencal herbs. Food Chem Toxicol 1996;34:449-456 https://doi.org/10.1016/0278-6915(96)00004-X
  5. Ann GW, Kang TW, Jeong JH, et al. Clinical studies on the irrtation effects of Mung Bean(Phaseolus aureus) extractin cosmatics. J Soc Cosmet Scientist Kor 2004;30:23-28
  6. Naksuriya O, Okonogi S, Schiffelers R, et al. Curcumin nanoformulations: A review of pharmaceutical properties and preclinical studies and clinical data related to cancer treatment. Biomaterials 2014;35:3365-3383 https://doi.org/10.1016/j.biomaterials.2013.12.090
  7. Araujo NC, Fontana CR, Bagnato VS, et al. Photodynamic effects of curcumin against cariogenic pathogens. Photomed Laser Surg 2012;30(7):393-399 https://doi.org/10.1089/pho.2011.3195
  8. Kim HS, Kang SM, Kwon HK, Kim BI. Antibacterial effect caused by the combination of xanthorrhizol and several surfactants. J Korean Acad Oral Health 2011;35(1):41-48
  9. Lee HJ : Photomechanical effects of Curcuma xanthorrhiza extract on Streptococcus mutans. Graduate school Yonsei University 2016.
  10. Heidi Abrahamse, Michael R. Hamblin. New photosensitizers for photodynamic therapy. Biochem J 2016;473(4):347-364 https://doi.org/10.1042/BJ20150942
  11. Kang SM : Optical identification and lethal photosensitization of oral bacteria. Graduate school Yonsei University 2016.
  12. 권필승. 포토프린을 이용한 황색포도알균과 표피포도알균에 대한 광역학 치료의 항균효과. 한국콘텐츠학회논문지 2016;13(2):314-321 https://doi.org/10.5392/JKCA.2013.13.02.314
  13. Choi SJ : The optimum parameters for the antimicrobial potential of photodynamic therapy to Streptococcus mutans biofilms. Graduate school Gangneung Wonju National University 2014.
  14. Wesley M, Cynthia M, Johan E. Photodynamic therapeutics: basic principles and clinical applications. Druq Discov Today 1999;4(11):507-517 https://doi.org/10.1016/S1359-6446(99)01412-9
  15. Zanin IC, Goncalves RB, Junior AB, Hope CK, Pratten J. Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 2005;56:324-330 https://doi.org/10.1093/jac/dki232
  16. Kim HD. Evaluation of possibility for hand disinfectant using ginger extract. Journal of Korean Society of Oral Health Science 2014;2(1):24-30
  17. Araujo CC, Leon LL. Biological activities of curcuma longa. Mem Inst Oswaldo Cruz 2001;96(5):723-728 https://doi.org/10.1590/S0074-02762001000500026
  18. Hanif R, Qiao L, Shiff SJ, Rigas B. Curcumin, a natural plant phenolic food additive, in hibits cell proliferation and induces cell cycle changes in colon adenocarcinoma cell lines by a prostaglandin independent pathway. J Lab Clin Med 1997;130(6):576-84 https://doi.org/10.1016/S0022-2143(97)90107-4
  19. Daliri F, Azizi A, Goudarzi M, Lawaf S, Rahimi A. In Vitro Comparison of the Effect of Photodynamic Therapy with Curcumin and Methylene Blue on Candida albicans Colonies. Photodiagnosis Photodyn Ther 2019;26:193-198 https://doi.org/10.1016/j.pdpdt.2019.03.017
  20. Ketan T. Savjani, Anuradha K. Gajjar, Jignasa K. Savjani. Drug Solubility: Importance and Enhancement Techniques. ISRN Pharm. 2012;195727:1-10
  21. Lee YN. Flora of Korea. Kyohaksa Co. 1996.
  22. Connell DW. The chemistry of the essential oil and oleoresin of ginger (Zingiber officinale Roscoe). Flavour Ind 1970;1:677-693
  23. Lee KW : Antibacterial activity of the zingiberaceae plant extract against oral microorganisms. Graduate school Yonsei University 2005.
  24. EC T, Yean-Kee L, Chin-Fei C, et al. Boesenbergia rotunda: From Ethnomedicine to Drug Discovery. Evidence-based Complementary and Alternative Medicine 2012;473637:1-25
  25. Choi SM. Dose prediction of fingerroot extract for anti-periodontitis by allometric scaling. Chung-Ang University 2018.
  26. HB Kim : Inhibitory Effects of Boesenbergia pandurata Extract and Its Active Compound Panduratin A on Inflammation and Alveolar Bone Loss Induced by Periodontitis. Graduate school Yonsei University 2018.
  27. Han SY, Kim BR, Ko HY, et al. Assessing the use of Quantitative Light-induced Fluorescence-Digital as a clinical plaque assessment. Photodiagnosis Photodyn Ther 2016;13:34-39 https://doi.org/10.1016/j.pdpdt.2015.12.002
  28. Lee ES, Kang SM, Ko HY, et al. Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D). J Dent 2013;41(12):1264-1270 https://doi.org/10.1016/j.jdent.2013.08.021
  29. Lee ES, De Jong EDJ, Jung HI, Kim BI. Red fluorescence of dental biofilm as an indicator for assessing the efficacy of antimicrobials. Journal of biomedical optics 2018;23(1):015003