Acknowledgement
이 연구는 2018학년도 건국대학교의 연구년교원 지원과 농촌진흥청 연구사업(세부과제번호: PJ012550032019)의 지원에 의해 연구되었습니다. 이에 감사드립니다.
Journal of Korean Society of Environmental Engineers (대한환경공학회지)
- Volume 41 Issue 8
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- Pages.419-430
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- 2019
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- 1225-5025(pISSN)
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- 2383-7810(eISSN)
DOI QR Code
Isolation, Counting and Identification of Microorganisms from Elevator Button, ATM, and Smartphone Surface
접촉 작동식 기기(승강기 버튼, 현금인출기, 스마트폰) 표면에 존재하는 미생물의 분리, 계수 및 동정
- Kim, Ji Eun (Department of Biological Engineering, Konkuk university) ;
- Lee, Eun Bin (Department of Biological Engineering, Konkuk university) ;
- Yang, Yun Jeong (Department of Biological Engineering, Konkuk university) ;
- Kim, Woo Hyeong (Department of Biological Engineering, Konkuk university) ;
- Oh, Jung Min (Department of Biological Engineering, Konkuk university) ;
- Lim, Dae Yong (Department of Biological Engineering, Konkuk university) ;
- Park, Ye Darm (Department of Biological Engineering, Konkuk university) ;
- Choi, Min Suk (Department of Biological Engineering, Konkuk university) ;
- Lee, Chae Young (Department of Biological Engineering, Konkuk university) ;
- An, Yeon Soo (Department of Biological Engineering, Konkuk university) ;
- Song, Hak Jin (Department of Biological Engineering, Konkuk university) ;
- Yoon, Jae Yoon (Department of Biological Engineering, Konkuk university) ;
- Kim, Hyung Joo (Department of Biological Engineering, Konkuk university)
- 김지은 (건국대학교 생물공학과) ;
- 이은빈 (건국대학교 생물공학과) ;
- 양윤정 (건국대학교 생물공학과) ;
- 김우형 (건국대학교 생물공학과) ;
- 오정민 (건국대학교 생물공학과) ;
- 임대용 (건국대학교 생물공학과) ;
- 박예닮 (건국대학교 생물공학과) ;
- 최민석 (건국대학교 생물공학과) ;
- 이채영 (건국대학교 생물공학과) ;
- 안연수 (건국대학교 생물공학과) ;
- 송학진 (건국대학교 생물공학과) ;
- 윤재윤 (건국대학교 생물공학과) ;
- 김형주 (건국대학교 생물공학과)
- Published : 20190000
Abstract
ObjectivesThe purpose of this study was to identify and isolate bacteria on the surface of elevator buttons, ATM screens, and smartphone surfaces. Through this process, bacterial diversity and presence of pathogens on the surface were evaluated for the prospective public health and environment.MethodsBacterial samples collected from elevator buttons, ATM screen surfaces, and smartphone surfaces were analyzed their CFUs using LB agar plates. Unique colonies in the plates were isolated and identified by the 16S rRNA analysis. The diversity of the bacteria in the collected samples containing high number of CFU was monitored using the Denaturing Gradient Gel Electrophoresis (DGGE).Results and DiscussionAmong the instrument surfaces, the elevator buttons showed the highest CFU (average: 163±17 CFU/cm2). The samples from smartphone and ATM surfaces have 66±13 and 6±1 CFU, respectively. The CFU analysis also showed that the all collected samples had active bacterial species, and the 16S rRNA analysis indicated that pathogenic bacteria were existed on the surface on the buttons and screens. The DGGE analysis showed that higher diversity of bacterial species were found compare to the CFU analysis of the samples. This difference in the bacterial diversity is probably due to the difference in optimal cultivation conditions of each bacterial species in the samples.ConclusionsThe user interfaces of the elevators, ATMs, and smartphones contained high diversity of normal and pathogenic bacterial species. Thus attention must be paid to the transfer of pathogenic bacteria in children, pregnant women, immunocompromised patients.