Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Virus-like Particle Vaccine Containing Toxoplasma gondii Rhoptry Protein 13 Induces Protection against T. gondii ME49 Infection in Mice

  • Kang, Hae-Ji (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Chu, Ki-Back (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Lee, Su-Hwa (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Kim, Min-Ju (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Park, Hyunwoo (Health Park Co., Ltd.) ;
  • Jin, Hui (Health Park Co., Ltd.) ;
  • Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine)
  • Received : 2019.07.22
  • Accepted : 2019.09.23
  • Published : 2019.10.31
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Abstract

Toxoplasma gondii can infect humans worldwide, causing serious diseases in pregnant women and immunocompromised individuals. T. gondii rhoptry protein 13 (ROP13) is known as one of the key proteins involved in host cell invasion. In this study, we generated virus-like particles (VLPs) vaccine expressing T. gondii rhoptry ROP13 and investigated VLPs vaccine efficacy in mice. Mice immunized with ROP13 VLPs vaccine elicited significantly higher levels of T. gondii-specific IgG, IgG1, IgG2a, and IgA antibody responses following boost immunization and challenge infection, whereas antibody inductions were insignificant upon prime immunization. Differing immunization routes resulted in differing antibody induction, as intranasal immunization (IN) induced greater antibody responses than intramuscular immunization (IM) after boost and challenge infection. IN immunization induced significantly higher levels of IgG and IgA antibody responses from feces, antibody-secreting cells (ASCs), $CD4^+$ T, $CD8^+$ T cells and germinal center B cell responses in the spleen compared to IM immunization. Compared to IM immunization, IN immunization resulted in significantly reduced cyst counts in the brain as well as lesser body weight loss, which contributed to better protection. All of the mice immunized through either route survived, whereas all na?ve control mice perished. These results indicate that the ROP13 VLPs vaccine could be a potential vaccine candidate against T. gondii infection.

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References

  1. Dubey J, Tiao N, Gebreyes W, Jones J. A review of toxoplasmosis in humans and animals in Ethiopia. Epidemiol Infect 2012; 140: 1935-1938. https://doi.org/10.1017/S0950268812001392
  2. Dubey JP. Toxoplasmosis of Animals and Humans. 2nd ed. Boca Raton, USA. CRC press. 2010.
  3. Wang PY, Yuan ZG, Petersen E, Li J, Zhang XX, Li XZ, Li HX, Lv ZC, Cheng T, Ren D, Yang GL, Lin RQ, Zhu XQ. Protective efficacy of a Toxoplasma gondii rhoptry protein 13 plasmid DNA vaccine in mice. Clin.Vaccine Immunol 2012; 19: 1916-1920. https://doi.org/10.1128/CVI.00397-12
  4. Foroutan M, Ghaffarifar F, Sharifi Z, Dalimi A, Jorjani O. Rhoptry antigens as Toxoplasma gondii vaccine target. Clin Exp Vaccine Res 2019; 8: 4-26. https://doi.org/10.7774/cevr.2019.8.1.4
  5. Lee SH, Kang HJ, Lee DH, Kang SM, Quan FS. Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection. Vaccine 2018; 36: 5692-5700. https://doi.org/10.1016/j.vaccine.2018.08.016
  6. Lee SH, Kang HJ, Lee DH, Quan FS. Protective immunity induced by incorporating multiple antigenic proteins of Toxoplasma gondii into influenza virus-like particles. Front Immunol 2018; 9: 3073. https://doi.org/10.3389/fimmu.2018.03073
  7. Lee SH, Kim AR, Lee DH, Rubino I, Choi HJ, Quan FS. Protection induced by virus-like particles containing Toxoplasma gondii microneme protein 8 against highly virulent RH strain of Toxoplasma gondii infection. PLoS One 2017; 12: e0175644. https://doi.org/10.1371/journal.pone.0175644
  8. Kang HJ, Lee SH, Kim MJ, Chu KB, Lee DH, Chopra M, Choi HJ, Park H, Jin H, Quan FS. Influenza virus-like particles presenting both Toxoplasma gondii ROP4 and ROP13 enhance protection against T. gondii Infection. Pharmaceutics 2019; 11: 342. https://doi.org/10.3390/pharmaceutics11070342
  9. Yoshida T, Mei H, Dorner T, Hiepe F, Radbruch A, Fillatreau S, Hoyer BF. Memory B and memory plasma cells. Immunol Rev 2010; 237: 117-139. https://doi.org/10.1111/j.1600-065X.2010.00938.x
  10. McHeyzer-Williams LJ, McHeyzer-Williams MG. Antigen-specific memory B cell development. Annu Rev Immunol 2005; 23: 487-513. https://doi.org/10.1146/annurev.immunol.23.021704.115732
  11. Zhu J, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations. Annu Rev Immunol 2009; 28: 445-489. https://doi.org/10.1146/annurev-immunol-030409-101212
  12. Holdsworth SR, Kitching AR, Tipping PG. Th1 and Th2 T helper cell subsets affect patterns of injury and outcomes in glomerulonephritis. Kidney Int 1999; 55: 1198-1216. https://doi.org/10.1046/j.1523-1755.1999.00369.x

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