Parasites, Hosts and Diseases

  • 제57권5호
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  • Pages.531-536
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  • 2019
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016

  • Ma, Da-Won (Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention) ;
  • Lee, Myoung-Ro (Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention) ;
  • Hong, Sung-Hee (Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention) ;
  • Cho, Shin-Hyeong (Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention) ;
  • Lee, Sang-Eun (Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention)
  • 투고 : 2019.05.31
  • 심사 : 2019.09.09
  • 발행 : 2019.10.31
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초록

Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013-2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and ${\beta}-giardin$ genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the ${\beta}-giardin$ gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.

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참고문헌

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  6. Giardia duodenalis Virulence - “To Be, or Not To Be” vol.8, pp.4, 2019, https://doi.org/10.1007/s40475-021-00248-z