Parasites, Hosts and Diseases

  • 제57권5호
  • /
  • Pages.525-529
  • /
  • 2019
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Molecular Detection and Subtyping of Blastocystis in Korean Pigs

  • Paik, Seunghyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Jung, Byeong Yeal (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Lee, Haeseung (College of Veterinary Medicine, Kyungpook National University) ;
  • Hwang, Mi-Hye (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Han, Jee Eun (College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Tae-Hwan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Oh-Deog (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwak, Dongmi (College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 2019.09.25
  • 심사 : 2019.09.29
  • 발행 : 2019.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Blastocystis is one of the most commonly detected genera of protozoan parasites in the human intestines as well as the intestines of many other species such as pigs in several geographical regions worldwide. However, no studies have examined Blastocystis in pigs in Korea. In this study, PCR and nucleotide sequencing were performed to evaluate the genetic diversity and zoonotic potential of Blastocystis using pig fecal samples. We obtained 646 stool samples from groups of piglets, weaners, growers, finishers, and sows in Korea. A total of 390 Blastocystis-positive samples were identified, and the infection rate was 60.4%. The infection rates were significantly related to age and region. The 4 subtypes (STs) of Blastocystis confirmed by phylogenetic analysis were ST1, ST2, ST3, and ST5, indicating the high genetic diversity of Blastocystis in Korean pigs. ST5 was highly distributed in Korean pigs among detected STs in this study. Some sequences were closely related to those of Blastocystis isolated from humans. This is the first study of Blastocystis in pigs in Korea. Based on the results, Blastocystis is prevalent in Korean pigs. Although a small number of samples were obtained in some areas, the clinical development of Blastocystis infection in pigs and potential for human transmission should be further examined.

키워드

참고문헌

  1. Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S. Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. J Clin Microbiol 2003; 41: 967-975. https://doi.org/10.1128/JCM.41.3.967-975.2003
  2. Fayer R, Elsasser T, Gould R, Solano G, Urban J, Jr., Santin M. Blastocystis tropism in the pig intestine. Parasitol Res 2014; 113: 1465-1472. https://doi.org/10.1007/s00436-014-3787-y
  3. Ramirez JD, Sanchez LV, Bautista DC, Corredor AF, Florez AC, Stensvold CR. Blastocystis subtypes detected in humans and animals from Colombia. Infect Genet Evol 2014; 22: 223-228. https://doi.org/10.1016/j.meegid.2013.07.020
  4. Santin M, Gomez-Munoz MT, Solano-Aguilar G, Fayer R. Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals. Parasitol Res 2011; 109: 205-212. https://doi.org/10.1007/s00436-010-2244-9
  5. Yoshikawa H, Koyama Y, Tsuchiya E, Takami K. Blastocystis phylogeny among various isolates from humans to insects. Parasitol Int 2016; 65: 750-759. https://doi.org/10.1016/j.parint.2016.04.004
  6. Noel C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 2005; 43: 348-355. https://doi.org/10.1128/JCM.43.1.348-355.2005
  7. Cian A, El Safadi D, Osman M, Moriniere R, Gantois N, Benamrouz-Vanneste S, Delgado-Viscogliosi P, Guyot K, Li LL, Monchy S, Noel C, Poirier P, Nourrisson C, Wawrzyniak I, Delbac F, Bosc S, Chabe M, Petit T, Certad G, Viscogliosi E. Molecular epidemiology of Blastocystis sp. in various animal groups from two French zoos and evaluation of potential zoonotic risk. PLoS One 2017; 12: e0169659. https://doi.org/10.1371/journal.pone.0169659
  8. Wang W, Owen H, Traub RJ, Cuttell L, Inpankaew T, Bielefeldt-Ohmann H. Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia. Vet Parasitol 2014; 203: 264-269. https://doi.org/10.1016/j.vetpar.2014.04.006
  9. Song JK, Hu RS, Fan XC, Wang SS, Zhang HJ, Zhao GH. Molecular characterization of Blastocystis from pigs in Shaanxi province of China. Acta Trop 2017; 173: 130-135. https://doi.org/10.1016/j.actatropica.2017.06.014
  10. Navarro C, Dominguez-Marquez MV, Garijo-Toledo MM, Vega-Garcia S, Fernandez-Barredo S, Perez-Gracia MT, Garcia A, Borras R, Gomez-Munoz MT. High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. Vet Parasitol 2008; 153: 347-358. https://doi.org/10.1016/j.vetpar.2008.02.003
  11. Riabi TR, Mirjalali H, Haghighi A, Nejad MR, Pourhoseingholi MA, Poirier P, Delbac F, Wawrzyniak I, Zali MR. Genetic diversity analysis of Blastocystis subtypes from both symptomatic and asymptomatic subjects using a barcoding region from the 18S rRNA gene. Infect Genet Evol 2018; 61: 119-126. https://doi.org/10.1016/j.meegid.2018.03.026
  12. Barbosa CV, Barreto MM, Andrade RJ, Sodre F, d'Avila-Levy CM, Peralta JM, Igreja RP, de Macedo HW, Santos HLC. Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): prevalence and genetic diversity of Blastocystis subtypes. PLoS One 2018; 13: e0193860. https://doi.org/10.1371/journal.pone.0193860
  13. Betts EL, Gentekaki E, Thomasz A, Breakell V, Carpenter AI, Tsaousis AD. Genetic diversity of Blastocystis in non-primate animals. Parasitology 2018; 145: 1228-1234. https://doi.org/10.1017/S0031182017002347
  14. Li WC, Wang K, Gu Y. Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China. Parasit Vectors 2018; 11: 93. https://doi.org/10.1186/s13071-018-2671-5
  15. Thrusfield M. Veterinary Epidemiology. 3rd ed. Oxford, UK. Blackwell Publishing. 2005, pp 228-246.
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870-1874. https://doi.org/10.1093/molbev/msw054
  17. Lee H, Lee SH, Seo MG, Kim HY, Kim JW, Lee YR, Kim JH, Kwon OD, Kwak D. Occurrence and genetic diversity of Blastocystis in Korean cattle. Vet Parasitol 2018; 258: 70-73. https://doi.org/10.1016/j.vetpar.2018.06.010
  18. Laodim P, Intapan PM, Sawanyawisuth K, Laummaunwai P, Maleewong W. A hospital-based study of epidemiological and clinical data on Blastocystis hominis infection. Foodborne Pathog Dis 2012; 9: 1077-1082. https://doi.org/10.1089/fpd.2012.1177
  19. Trabelsi S, Ali IB, Khaled S. Clinical and epidemiological characteristics of Blastocystis hominis. Tunis Med 2010; 88: 190-192.
  20. Juarez MM, Rajal VB. Intestinal parasitoses in Argentina: major causal agents found in the population and in the environment. Rev Argent Microbiol 2013; 45: 191-204.
  21. Ruaux CG, Stang BV. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest. PLoS One 2014; 9: e107496. https://doi.org/10.1371/journal.pone.0107496

피인용 문헌

  1. First Report on the Occurrence and Subtypes of Blastocystis in Pigs in Poland Using Sequence-Tagged-Site PCR and Barcode Region Sequencing vol.9, pp.7, 2019, https://doi.org/10.3390/pathogens9070595
  2. First Report of Blastocystis Infection in Pigs from Large Farms in Xinjiang, China vol.67, pp.6, 2020, https://doi.org/10.1111/jeu.12816
  3. Molecular detection and subtype distribution of Blastocystis in farmed pigs in southern China vol.151, 2019, https://doi.org/10.1016/j.micpath.2021.104751
  4. Blastocystis in domesticated and wild mammals and birds vol.135, 2019, https://doi.org/10.1016/j.rvsc.2020.09.031
  5. Prevalence and Subtype Distribution of Blastocystis sp. in Diarrheic Pigs in Southern China vol.10, pp.9, 2021, https://doi.org/10.3390/pathogens10091189
  6. The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010-2021 in Asia vol.10, pp.10, 2019, https://doi.org/10.3390/biology10100990
  7. Molecular prevalence and subtype distribution of Blastocystis sp. in Asia and in Australia vol.19, pp.5, 2019, https://doi.org/10.2166/wh.2021.011
  8. Global prevalence, subtypes distribution, zoonotic potential, and associated risk factors of Blastocystis sp. in domestic pigs (Sus domesticus) and wild boars (Sus scrofa): A systematic review and met vol.160, 2021, https://doi.org/10.1016/j.micpath.2021.105183