Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Proteomic Analysis on Exosomes Derived from Patients, Sera Infected with Echinococcus granulosus

  • Wang, Wen (School of Basic Medicine, Ningxia Medical University) ;
  • Zhou, Xiaojing (College of Clinical Medicine, Ningxia Medical University) ;
  • Cui, Fang (School of Basic Medicine, Ningxia Medical University) ;
  • Shi, Chunli (School of Basic Medicine, Ningxia Medical University) ;
  • Wang, Yulan (College of Clinical Medicine, Ningxia Medical University) ;
  • Men, Yanfei (College of Clinical Medicine, Ningxia Medical University) ;
  • Zhao, Wei (School of Basic Medicine, Ningxia Medical University) ;
  • Zhao, Jiaqing (School of Basic Medicine, Ningxia Medical University)
  • Received : 2019.04.24
  • Accepted : 2019.09.15
  • Published : 2019.10.31
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Abstract

Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.

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