Journal of Medicine and Life Science

Institute for Medical Science (제주대학교 의과학연구소)
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How to design male hypothyroid hypogonadism model

갑상샘기능저하증에 따른 생식샘기능저하증 모델 정립을 위한 제언

  • Yoon, Sang-Pil (Department of Anatomy, School of Medicine, Jeju National University) ;
  • Kim, Jung Woo (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • 윤상필 (제주대학교 의과대학 해부학교실) ;
  • 김정우 (원광보건대학교 임상병리학과)
  • Received : 2019.04.04
  • Accepted : 2019.04.16
  • Published : 2019.04.30
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Abstract

Hypogonadism is a clinical syndrome that results in hormone deficiency and can be classified as 1) primary caused by the gonadal failure and 2) secondary by the hypothalamus-pituitary gland dysfunction and/or cardiometabolic complications. Recently the presence of thyroid hormone receptors in different testicular cell types was demonstrated, and thus thyroid dysfunctions would be another cause of secondary hypogonadism. Thus, we investigated the effects of perinatal hypothyroidism on hypogonadism in male Sprague-Dawley rats. Perinatal hypothyroidism was induced by daily administration of 0.05% 6-propyl-2-thiouracil (PTU) by tap water from gestation day 15, which were compared with negative control (PTU (-)) group. At postnatal day 28, hypothyroid pups were divided into 2 groups: PTU (+) group - continued PTU treatment and PTU (+/-) group - stopped PTU until postnatal day 49. Body weights, dehydrotesosterone (DHT), and testosterone levels were checked 2 and 3 weeks after grouping. Body weights were significantly decreased in PTU(+) and PTU(+/-) groups compared with PTU (-) group at postnatal day 28. 3 weeks later, PTU (+/-) group significantly gained weight compared with PTU (+) group. DHT and testosterone levels significantly decreased with PTU treatment, but increased 3 weeks after stopping PTU administration. Perinatal PTU-induced hypothyroid hypogonadism was sustained for 2 weeks after stopping PTU administration, but restored gonadal hormone levels 3 weeks after stopping PTU. These results suggest that researchers should design an experiment on hypothyroid hypogonadism based on the estimated period.

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