Antioxidant, Whitening, Anti-inflammatory Effect of Supercritical Centella asiatica Extract and Enhancement of Skin Permeation Using Epidermal Penetrating Peptide

초임계 병풀 추출물의 항산화, 미백, 항염증 효과와 경피투과 펩티드를 이용한 피부흡수 증진

The purpose of this study is to investigate antioxidant, whitening and anti-inflammatory effects of Centella asiatica extracts using supercritical extraction. In addition, Franz diffusion cell experiment was conducted using polymer micelle and cell permeation peptide for transdermal delivery. The DPPH radical scavenging test showed the highest DPPH radiated scavenging of C. asiatica water extract (CWE) and the SOD-like activity experiment represented that C. asiatica ethanol extract (CEE) was the highest SOD-like activity. However, C. asiatica supercritical extract (CSE) also showed good results in DPPH radical scavenging and SOD-like activity. In the inhibition test of melanin induced by α-MSH in B16 cells, CWE & CEE showed melanin inhibiting at high concentrations, but less than 20% at concentrations below 200 ㎍/mL. CSE represented melanin inhibiting at less than 50 ㎍/mL and almost 50% inhibition at the concentration. The inhibitory results of NO (nitric oxide) generation induced by LPS showed 88% by CWE at a concentration of 800 ㎍/mL compared to control treated only by LPS, 13% by CEE at a concentration of 200 ㎍/mL, 71% by CSE at a concentration of 100 ㎍/mL, and concentration-dependent increases in inhibits were found in CWE & CSE. For the skin permeability, in vitro experiment was conducted using polymer micelle and cell permeation peptide (R6) using the indicator material for CSE. The experiment showed that polymer micelle and cell permeation peptide effectively helped permeate the percutaneous tissue of CSE.