Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The bactericidal effect of an atmospheric-pressure plasma jet on Porphyromonas gingivalis biofilms on sandblasted and acid-etched titanium discs

  • Lee, Ji-Yoon (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Kim, Kyoung-Hwa (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Park, Shin-Young (Program of Clinical Dental Education and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Yoon, Sung-Young (Plasma Technology Research Center, National Fusion Research Institute) ;
  • Kim, Gon-Ho (Department of Energy Systems (Nuclear) Engineering, Seoul National University School of Engineering) ;
  • Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry) ;
  • Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry)
  • Received : 2019.06.24
  • Accepted : 2019.08.28
  • Published : 2019.10.30
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Abstract

Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.

Keywords

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