Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Study of Root Characteristics of Angelica gigas Nakai in Plantations using a Micro-CT Camera

Micro-CT 촬영기법에 의한 재배지 참당귀의 뿌리 특성

  • Jeong, Dae Hui (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Kim, Ki Yoon (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Sim, Su Jin (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Jung, Chung Ryul (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Kim, Hyun Jun (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Jeon, Kwon Seok (Forest Medicinal Resources Research Center, Korea Forest Service) ;
  • Park, Hong Woo (Forest Medicinal Resources Research Center, Korea Forest Service)
  • 정대희 (국립산림과학원 산림약용자원연구소) ;
  • 김기윤 (국립산림과학원 산림약용자원연구소) ;
  • 심수진 (국립산림과학원 산림약용자원연구소) ;
  • 정충렬 (국립산림과학원 산림약용자원연구소) ;
  • 김현준 (국립산림과학원 산림약용자원연구소) ;
  • 전권석 (국립산림과학원 산림약용자원연구소) ;
  • 박홍우 (국립산림과학원 산림약용자원연구소)
  • Received : 2019.07.16
  • Accepted : 2019.09.08
  • Published : 2019.10.30
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Abstract

Background: We measured the weight characteristics, intercellular space volume as a proportion of root volume, and quantities of the functional component in Angelica gigas Nakai, collected from four cultivation areas in Pyeongchang, including Tapdong-ri and SangjinBu-ri, and tested the results for correlations. Methods and Results: Dry weight was the highest in the Tapdong-ri group ($13.37{\pm}0.13g$) and the lowest in the SangjinBu-ri group ($11.90{\pm}0.57g$). The percentage of intercellular spaces within the roots, determined by micro-CT camera, was the highest in the Tapdong-ri group ($6.44{\pm}0.76%$) and the lowest in the SangjinBu-ri group ($9.01{\pm}2.15%$). The quantity of functional components were the highest in the Tapdong-ri group ($116.13{\pm}9.53mg/g$) and the lowest in the SangjinBu-ri group ($99.77{\pm}23.23mg/g$). Conclusions: The dry weight of A. gigas significantly correlated with the volume and percentage of intercellular space. An increase in the dry weight resulted in a negative correlation between the volume and ratio of the intercellular spaces. The volume of intercellular space correlated negatively with levels of the functional components, this was significant in the case of the nodakenin, decursin and decursinol angelate content (p < 0.016).

Keywords

References

  1. Ahn MJ, Bae JY and Park JH. (2011). Pharmacognostical studies on the Angelica species from Korea. Korean Journal of Pharmacognosy. 42:1-4.
  2. Buie HR, Campbell GM, Klinck RJ, MacNeil JA and Boyd SK. (2007). Automatic segmentation of cortical and trabecular compartments based on a dual threshold technique for in vivo micro-CT bone analysis. Bone. 41:505-515. https://doi.org/10.1016/j.bone.2007.07.007
  3. Burr-Hersey JE, Mooney SJ, Bengough AG, Mairhofer S and Ritz K. (2017). Developmental morphology of cover crop species exhibit contrasting behaviour to changes in soil bulk density, revealed by X-ray computed tomography. PLoS ONE. 12:e0181872. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181872 (cited by 2019 May 15).
  4. Dejaco A, Komlev VS, Jaroszewicz J, Swieszkowski W and Hellmich C. (2012). Micro CT-based multiscale elasticity of double-porous(pre-cracked) hydroxyapatite granules for regenerative medicine. Journal of Biomechanics. 45:1068-1075. https://doi.org/10.1016/j.jbiomech.2011.12.026
  5. Gainkam LOT, Huang L, Caveliers V, Keyaerts M, Hernot S, Vaneycken I, Vanhove C, Revets H, de Baetselier P and Lahoutte T. (2008). Comparison of the biodistribution and tumor targeting of two 99mTc-Labeled anti-EGFR nanobodies in mice, using pinhole SPECT/Micro-CT. Journal of Nuclear Medicine. 49:788-795. https://doi.org/10.2967/jnumed.107.048538
  6. Geumsan Agricultural Technology Service Center(GATSC). (2005). Analysis of ginseng root growth using neutron radiography and visualization of water distribution in processed ginseng using neutron tomography. Geumsan Agricultural Technology Service Center. Geumsan, Korea. p.1-34.
  7. Geumsan Ginseng and Medicinal Crop Experiment Station (GGMCES). (2005). 3-dimentional visualization of ginseng root growth at main growth stage in soil using neutron tomography. Geumsan Ginseng and Medicinal Crop Experiment Station. Geumsan, Korea. p.1-35.
  8. Jang CS and Oh BU. (2016). A taxonomic study of Korean Juncus based on the anatomical characters. Korean Journal of Plant Taxonomy. 46:392-404. https://doi.org/10.11110/kjpt.2016.46.4.392
  9. Jeong HJ, Hong SY, Lee KY, Lee JH, Lim SH, Heo NK and Kim HY. (2015). Diabetic effect of Angelica gigas Nakai leaf extracts in diabetes mellitus type II model mice by high fat-fed and streptozotocin-treated rats. Journal of Agricultural, Life and Environmental Sciences. 27:6-14.
  10. Jung YM. (2015). The function of nutrient. In the prevention and minerals for Korea agriculture in the future. Gomundang printing Inc., Daegu, Korea. p.101-117.
  11. Karahara I, Yamauchi D, Uesugi K and Mineyuki Y. (2015). Three-dimensional imaging of plant tissues using X-ray microcomputed tomography. Plant Morphology. 27:21-26. https://doi.org/10.5685/plmorphol.27.21
  12. Kim JW, Kim CH, Back JH, Lee DJ, Yong CS and Heo SS. (2014a). Introduction of soil fertilizer. Seonjinmunhwasa. Goyang, Korea. p.207-213.
  13. Kim JY, Yoon YD, Ahn JM, Kang JS, Park SK, Lee K, Song KB, Kim HM and Han SB. (2007). Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4. International Immunopharmacology. 7:78-87. https://doi.org/10.1016/j.intimp.2006.08.017
  14. Kim SA, Oh HK, Kim JY, Hong JW and Cho SI. (2011). A review of pharmacological effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their bioactive compounds. Journal of Korean Oriental Medicine. 32:1-24.
  15. Kim YA, Park SH, Kim BY, Kim AH, Park BJ and Kim JJ. (2014b). Inhibitory effects on melanin production of demethylsubersin isolated from Angelica gigas Nakai. Korean Journal of Pharmacognosy. 45:209-213.
  16. Kim YG, An TJ, Yeo JH, Hur M, Park YS, Cha SW, Song BH and Lee KA. (2014c). Effects of eco-friendly organic fertilizer on growth and yield of Angelica gigas Nakai. Korean Journal of Medical Crop Science. 22:127-133. https://doi.org/10.7783/KJMCS.2014.22.2.127
  17. Kwon JH, Han MS, Lee BM and Lee YM. (2015). Effect of Angelica gigas extract powder on progress of osteoarthritis induced by monosodium iodoacetate in rats. Analytical Science and Technology. 28:72-77. https://doi.org/10.5806/AST.2015.28.1.72
  18. Lee MY, Im SH, Ju YS, Han HS, Jeong HJ, An DG, Kang HC and Ko BS. (2000). Discrimination of three Angelica species using the RAPDs and internal root structure. Korean Journal of Medical Crop Science. 8:243-249.
  19. Lee SH, Shin DS, Kim JS, Oh KB and Kang SS. (2003). Antibacterial coumarins from Angelica gigas roots. Archibes of Pharmacal Research. 26:449-452. https://doi.org/10.1007/BF02976860
  20. Lee TB. (2003). Coloured flora of Korea. Vol. 1. Hayangmunsa. Seoul, Korea. p.839-844.
  21. Lee YN. (2006). New flora of Korea. Vol. 1. Kyohak Publising Co. Seoul, Korea. p.810-832.
  22. Mathers AW, Hepworth C, Baillie AL, Sloan J, Jones H, Lundgren M, Fleming AJ, Mooney SJ and Sturrock CJ. (2018). Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography. Plant Methods. 14:99. https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-018-0367-7 (cited by 2019 June 3).
  23. Moon YJ and Jang SA. (2011). Quality characteristics of cookies containing powder of extracts from Angelica gigas Nakai. Korean Journal of Food and Nutrition. 24:173-179. https://doi.org/10.9799/ksfan.2011.24.2.173
  24. Nam HI and Baik TH. (2016). Inhibitory effects of Angelica gigas Nakai on ulcerative colitis in dSS-induced ICR Mice. Journal of Physiology and Pathology in Korean Medicine. 30:439-446. https://doi.org/10.15188/kjopp.2016.12.30.6.439
  25. Paquita VC, Gleasona SS and Kalluri UC. (2011). Monitoring plant growth using high resolution micro-CT images. Proceedings of SPIE-IS&T Electronic Imaging. 7877:78770W. https://doi.org/10.1117/12.876719 (cited by 2019 May 19).
  26. Park MJ, Kang SJ and Kim AJ. (2009a). Hypoglycemic effect of Angelica gigas Nakai extract in streptozotocin-induced diabetic rats. Korean Journal of Food and Nutrition. 22:246-251.
  27. Park SK, Hong SK, Kim HJ, Kim BY, Kim TG, Kang JS and Kim DU. (2009b). Cosmetic effect of Angelica gigas Nakai root extracts. Korean Chemical Engineering Research. 47:553-557.
  28. Park YM, Jeong DH, Sim SJ, Kim NH, Park HW and Jeon GS. (2019). The characteristics of growth and active compounds of Angelica gigas Nakai population in Mt. Jeombong. Korean Journal of Plant Resources. 32:9-18. https://doi.org/10.7732/KJPR.2019.32.1.009
  29. Ruzin ES. (1999). Plant microtechnique and morphology. Oxford University Press. Oxford, England. p.127-128.
  30. Seo BI, Lee GH, Chol HY, Kwon DR and Boo YM. (2008). Chinese medicine herbalogy. Yeongnimsa. Seoul, Korea. p.851-857.
  31. Sim CM, Seong BJ, Kim DW, Kim YB, Wi SG, Kim GI, Oh HS, Kim TJ, Chung BY, Song JY, Kim HG, Oh SK, Shin YD, Seok JH, Kang MY, Lee YH, Radebe MJ, Kardjilov N and Honermeier B. (2018). Continuous cropping of endangered therapeutic plants via electron beam soil-treatment and neutron tomography. Scientific Report. 8:2136. https://www.nature.com/articles/s41598-018-20124-7.pdf (cited by 2019 May 20). https://doi.org/10.1038/s41598-018-20124-7
  32. Son SH, Kim MJ, Chung WY, Son JA, Kim YS, Kim YC, Kang SS, Lee SK and Park KK. (2009). Decursin and decursinol inhibit VEGF-induced angiogenesis by blocking the activation of extracellular signal-regulated kinase and c-Jun Nterminal kinase. Cancer Letters. 280:86-92. https://doi.org/10.1016/j.canlet.2009.02.012
  33. Song JH and Hong SP. (2012). Comparative anatomy of petiole in Forsythieae, Fontanesieae and Myxopyrum(Oleaceae) and its systematic implication. Korean Journal of Plant Taxonomy. 42:50-63. https://doi.org/10.11110/kjpt.2012.42.1.050
  34. Sung JS, Bang KH, Park CH, Park CG, Yu HS, Park HW and Seong NS. (2004). Discrimination of Angelicae Radix based on anatomical characters. Korean Journal of Medical Crop Science. 12:67-72.
  35. Torres-Ruiz JM, Jansen S, Choat SB, Mcelrone AJ, Cochard H, Brodribb TJ, Badel E, Burlett R, Bouche PS, Brodersen CR, Li S, Morris H and Delzon S. (2015). Direct X-ray microtomography observation confirms the induction of embolism upon xylem cutting under tension. Journal of Plant Physiology. 167:40-43. https://doi.org/10.1104/pp.114.249706
  36. Tuan HS and Hutmacher DW. (2005). Application of micro CT and computation modeling in bone tissue engineering. Computer Aided Design. 37:1151-1161. https://doi.org/10.1016/j.cad.2005.02.006
  37. Yoon MY, Kim YS, Choi GJ, Jang KS, Choi YH, Cha BJ and Kim JC. (2011). Antifungal activity of decursinol angelate isolated from Angelica gigas roots against Puccinia recondite. Research in Plant Disease. 17:21-31.
  38. Yu HS, Jo JS, Park CH, Park CG, Sung JS, Park HW, Seong NS and Jin DC. (2003). Plant growth and bolting affected by transplanting time in Angelica gigas. Korean Journal of Medical Crop Science. 11:392-396.