한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제23권1호
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  • Pages.35-42
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  • 2019
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Expression Patterns of Growth Related Genes in Juvenile Red Spotted Grouper (Epinephelus akaara) with Different Growth Performance after Size Grading

  • Mun, Seong Hee (Dept. of Aquatic Life Medical Science, Sunmoon University) ;
  • You, Jin Ho (Dept. of Aquatic Life Medical Science, Sunmoon University) ;
  • Oh, Hyeon Ji (Dept. of Aquatic Life Medical Science, Sunmoon University) ;
  • Lee, Chi Hoon (CR Co., Ltd.) ;
  • Baek, Hea Ja (Dept. of Marine Biology, Pukyong National University) ;
  • Lee, Young-Don (Dept. of Marine Science Institute, Jeju National University) ;
  • Kwon, Joon Yeong (Dept. of Aquatic Life Medical Science, Sunmoon University)
  • 투고 : 2019.01.14
  • 심사 : 2019.03.09
  • 발행 : 2019.03.31
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초록

Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper (Epinephelus akaara) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature $24{\pm}1^{\circ}C$, natural light) for 96 days after hatching. They were divided into 3 groups by size (fast growing, middle growing and slow growing groups: FGG, MGG, and SGG, respectively). RNA was extracted from the brain, liver and muscle tissues from each group, and target gene expression was examined by real-time PCR. In the brain with pituitary gland, expression of GH gene in FGG was significantly higher than the expression in SGG, but the expression of IGF1 and GHR genes in the muscle was highest in SGG. Difference of GHR and IGF1 mRNA in the liver between groups with different growth performance was less clear than that in other tissues, although level of IGF1 mRNA was higher in SGG than in MGG. These results suggest that hormonal governing of growth is not the same in fast growing and slow growing fish, and size grading could cause a shift of hormonal state and growth pattern in this species.

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참고문헌

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